Increased formation of phosphorylated H2AX foci in nuclei of cells infected by hepatitis B AND B+D viruses
Костюшев Д. С., Брезгин С., Акостюшева А. П., Липатников А. Д., Симирскил В. Н., Мамонова Н. А., Волчкова Е. В., Малеьев В. В., Чуланов В. П.
Вопросы вирусологии
Т. 63, Вып. 4, С. 165-170
Опубликовано: 2018
Тип ресурса: Статья
DOI:10.18821/0507-4088-2018-63-4-165-170
Аннотация:
Liver cirrhosis and hepatocellular carcinoma are the most common outcomes of chronic hepatitis B. Hepatitis B virus (HBV) induces transformation and cell death in chronic hepatitis B (CHB). DNA double strand breaks (DSBs) represent the most dangerous type of genome damage. It was shown previously that generation of phosphorylated histone H2AX foci is a reliable marker of DSBs. The aim of this study was to analyse generation of yH2AX foci in HBV and hepatitis D virus (HDV) infection in vitro and in liver biopsies of patients with CHB and CHB with delta-agent (CHD). Human hepatoma cell line HepG2-1.1merHBV with activated HBV life cycle was used to perform real-time PCR for analysis of pregenomic RNA, HBV DNA, HBV cccDNA and for immunocytochemical analysis of yH2AX. Liver biopsies from CHB and CHD patients were analyzed to confirm the results. HBV induces multiple discrete yH2AX foci in HepG2-1.1merHBV cells in vitro and in biopsies of CHB and CHB+D patients. The ratio of hepatocytes w/o
Ключевые слова:
Double strand breaks; Genome damage; Hepatitis b virus; Hepatitis d virus; Hepatocytes; Hepatoma cells; Yh2ax
Язык текста: Русский
ISSN: 2411-2097
Костюшев Д. С.
Брезгин С.
Акостюшева А. П.
Липатников А. Д.
Симирскил В. Н.
Мамонова Н. А.
Волчкова Е. В. Елена Васильевна 1953-
Малеьев В. В.
Чуланов В. П. Владимир Петрович 1970-
Kostyushev D. S.
Brezgin S.
Akostyusheva A. P.
Lipatnikov A. D.
Simirskil V. N.
Mamonova N. A.
Volchkova E. V. Elena Vasilyevna 1953-
Maleyev V. V.
Chulanov V. P. Vladimir Petrovich 1970-
Increased formation of phosphorylated H2AX foci in nuclei of cells infected by hepatitis B AND B+D viruses
Текст визуальный непосредственный
Вопросы вирусологии
Издательство "Медицина"
Т. 63, Вып. 4 С. 165-170
2018
Статья
Double strand breaks Genome damage Hepatitis b virus Hepatitis d virus Hepatocytes Hepatoma cells Yh2ax
Liver cirrhosis and hepatocellular carcinoma are the most common outcomes of chronic hepatitis B. Hepatitis B virus (HBV) induces transformation and cell death in chronic hepatitis B (CHB). DNA double strand breaks (DSBs) represent the most dangerous type of genome damage. It was shown previously that generation of phosphorylated histone H2AX foci is a reliable marker of DSBs. The aim of this study was to analyse generation of yH2AX foci in HBV and hepatitis D virus (HDV) infection in vitro and in liver biopsies of patients with CHB and CHB with delta-agent (CHD). Human hepatoma cell line HepG2-1.1merHBV with activated HBV life cycle was used to perform real-time PCR for analysis of pregenomic RNA, HBV DNA, HBV cccDNA and for immunocytochemical analysis of yH2AX. Liver biopsies from CHB and CHD patients were analyzed to confirm the results. HBV induces multiple discrete yH2AX foci in HepG2-1.1merHBV cells in vitro and in biopsies of CHB and CHB+D patients. The ratio of hepatocytes w/o