Development of nNOS-positive preganglionic sympathetic neurons in the rat thoracic spinal cord
Moiseev K. Y., Romanova I. V., Masliukov A. P., Masliukov P. M.
Cell and Tissue Research
Vol.375, Issue2, P. 345-357
Опубликовано: 2019
Тип ресурса: Статья
DOI:10.1007/s00441-018-2925-8
Аннотация:
To gain a better understanding of the neuroplasticity of sympathetic neurons during postnatal ontogenesis, the distribution of neuronal nitric oxide synthase (nNOS) immunoreactivity was studied in sympathetic preganglionic neurons (SPN) in the spinal cord (Th2 segment) of female Wistar rats at different ages (newborn, 10-, 20-, 30-day-old; 2-, 6-month-old; 3-year-old). In all age groups, the majority of nNOS-immunoreactive (IR) neurons was observed in the nucleus intermediolateralis thoracolumbalis pars principalis. In the first month, the proportion of nNOS-IR neurons decreased significantly from 92 ± 3.4[%] in newborn to 55 ± 4.6[%] in 1-month-old, while the number of choline acetyltransferase (ChAT)-IR neurons increased from 74 ± 4.2[%] to 99 ± 0.3[%] respectively. Decreasing nNOS expression in the first 10 days of life was also confirmed by western blot analysis. Some nNOS-IR SPN also colocalized calbindin (CB) and cocaine and amphetamine-regulated transcript (CART). The percentage of NOS(
Ключевые слова:
Development; Immunohistochemistry; Nitric oxide synthase; Spinal cord; Sympathetic preganglionic neurons
calbindin; choline acetyltransferase; cocaine and amphetamine regulated transcript peptide; neuronal nitric oxide synthase; calbindin; choline acetyltransferase; cocaine and amphetamine regulated transcript peptide; nerve protein; neuronal nitric oxide synthase; age distribution; animal experiment; animal tissue; Article; autonomic nervous system; controlled study; female; immunohistochemistry; immunoreactivity; nervous system development; newborn; nonhuman; nucleus intermediolateralis thoracolumbalis pars principalis; preganglionic nerve cell; priority journal; protein expression; protein localization; rat; senescence; sympathetic ganglion; sympathetic nerve cell; thoracic spinal cord; Western blotting; adrenergic system; animal; cytology; metabolism; preganglionic nerve; spinal cord lateral horn; thoracic vertebra; Wistar rat; Animals; Animals, Newborn; Autonomic Fibers, Preganglionic; Calbindins; Choline O-Acetyltransferase; Female; Nerve Tissue Proteins; Nitric Oxide Synthase Type
Язык текста: Английский
ISSN: 1432-0878
Moiseev K. Y.
Romanova I. V.
Masliukov A. P.
Masliukov P. M.
Моисеев К. Y.
Романова И. В.
Маслиуков А. П.
Маслиуков П. М.
Development of nNOS-positive preganglionic sympathetic neurons in the rat thoracic spinal cord
Текст визуальный непосредственный
Cell and Tissue Research
Springer-Verlag GmbH
Vol.375, Issue2 P. 345-357
2019
Статья
Development Immunohistochemistry Nitric oxide synthase Spinal cord Sympathetic preganglionic neurons
calbindin choline acetyltransferase cocaine and amphetamine regulated transcript peptide neuronal nitric oxide synthase calbindin choline acetyltransferase cocaine and amphetamine regulated transcript peptide nerve protein neuronal nitric oxide synthase age distribution animal experiment animal tissue Article autonomic nervous system controlled study female immunohistochemistry immunoreactivity nervous system development newborn nonhuman nucleus intermediolateralis thoracolumbalis pars principalis preganglionic nerve cell priority journal protein expression protein localization rat senescence sympathetic ganglion sympathetic nerve cell thoracic spinal cord Western blotting adrenergic system animal cytology metabolism preganglionic nerve spinal cord lateral horn thoracic vertebra Wistar rat Animals Animals, Newborn Autonomic Fibers, Preganglionic Calbindins Choline O-Acetyltransferase Female Nerve Tissue Proteins Nitric Oxide Synthase Type
To gain a better understanding of the neuroplasticity of sympathetic neurons during postnatal ontogenesis, the distribution of neuronal nitric oxide synthase (nNOS) immunoreactivity was studied in sympathetic preganglionic neurons (SPN) in the spinal cord (Th2 segment) of female Wistar rats at different ages (newborn, 10-, 20-, 30-day-old; 2-, 6-month-old; 3-year-old). In all age groups, the majority of nNOS-immunoreactive (IR) neurons was observed in the nucleus intermediolateralis thoracolumbalis pars principalis. In the first month, the proportion of nNOS-IR neurons decreased significantly from 92 ± 3.4[%] in newborn to 55 ± 4.6[%] in 1-month-old, while the number of choline acetyltransferase (ChAT)-IR neurons increased from 74 ± 4.2[%] to 99 ± 0.3[%] respectively. Decreasing nNOS expression in the first 10 days of life was also confirmed by western blot analysis. Some nNOS-IR SPN also colocalized calbindin (CB) and cocaine and amphetamine-regulated transcript (CART). The percentage of NOS(