Synthetic CpG oligonucleotides as potential modulators of neutrophil survival in PAMP-associated inhibition of apoptosis
Golenkina E. A., Viryasova G. M., Galkina S. I., Arifulin E. A., Gaponova T. V., Romanova Yu. M., Sud'ina G. F.
Journal of Leukocyte Biology
Vol.106, Issue1, P. 45-55
Опубликовано: 2019
Тип ресурса: Статья
DOI:10.1002/JLB.3MIA1118-435R
Аннотация:
We studied the effects of a synthetic CpG oligonucleotide (CpG ODN2006) on polymorphonuclear leukocyte (PMNL, neutrophil) survival and oxidant status. CpG ODN2006 showed a dose-dependent effect on the apoptosis of resting neutrophils. Without affecting the viability of resting cells, low concentrations of CpG ODN2006 interfered with Salmonella typhimurium-mediated viability prolongation and increased neutrophil apoptosis to control levels. CpG ODN2006 stimulated neutrophil apoptosis by enhancing ROS generation. Even small doses of ODN could induce the production of intracellular superoxide anions. The high superoxide reactogenicity, including with respect to nitrogen oxide, led to increased levels of intracellular ROS and RNS, which ultimately caused apoptosis. The pro-oxidant effect of low concentrations of CpG ODN2006 was not sufficient to trigger irreversible pro-apoptotic mechanisms. However, the sensitivity of PMNLs to ODN2006, a modulator of apoptosis, increased significantly und
Ключевые слова:
apoptosis; CpG oligonucleotide; neutrophil; reactive nitrogen species; reactive oxygen species; Salmonella
CpG oligodeoxynucleotide; nitric oxide; nitrogen oxide; reactive oxygen metabolite; superoxide; nitric oxide; ODN2006; oligodeoxyribonucleotide; pathogen associated molecular pattern; reactive oxygen metabolite; apoptosis; Article; cell survival; controlled study; CpG island; DNA fragmentation; electric potential; flow cytometry; high performance liquid chromatography; human; human cell; in vitro study; neutrophil; nonhuman; priority journal; Salmonella enterica serovar Typhimurium; salmonellosis; signal transduction; apoptosis; drug effect; metabolism; neutrophil; physiology; Apoptosis; Humans; Neutrophils; Nitric Oxide; Oligodeoxyribonucleotides; Pathogen-Associated Molecular Pattern Molecules; Reactive Oxygen Species; Salmonella typhimurium
Язык текста: Английский
ISSN: 1938-3673
Golenkina E. A.
Viryasova G. M.
Galkina S. I.
Arifulin E. A.
Gaponova T. V.
Romanova Yu. M. Yuliya Mikhaylovna 1946-
Sud'ina G. F.
Голенкина Е. А.
Вирясова Г. М.
Галкина С. И.
Арифулин Е. А.
Гапонова Т. В.
Романова Ю. М. Юлия Михайловна 1946-
Суд'ина Г. Ф.
Synthetic CpG oligonucleotides as potential modulators of neutrophil survival in PAMP-associated inhibition of apoptosis
Текст визуальный непосредственный
Journal of Leukocyte Biology
Federation of American Societies for Experimental Biology
Vol.106, Issue1 P. 45-55
2019
Статья
apoptosis CpG oligonucleotide neutrophil reactive nitrogen species reactive oxygen species Salmonella
CpG oligodeoxynucleotide nitric oxide nitrogen oxide reactive oxygen metabolite superoxide nitric oxide ODN2006 oligodeoxyribonucleotide pathogen associated molecular pattern reactive oxygen metabolite apoptosis Article cell survival controlled study CpG island DNA fragmentation electric potential flow cytometry high performance liquid chromatography human human cell in vitro study neutrophil nonhuman priority journal Salmonella enterica serovar Typhimurium salmonellosis signal transduction apoptosis drug effect metabolism neutrophil physiology Apoptosis Humans Neutrophils Nitric Oxide Oligodeoxyribonucleotides Pathogen-Associated Molecular Pattern Molecules Reactive Oxygen Species Salmonella typhimurium
We studied the effects of a synthetic CpG oligonucleotide (CpG ODN2006) on polymorphonuclear leukocyte (PMNL, neutrophil) survival and oxidant status. CpG ODN2006 showed a dose-dependent effect on the apoptosis of resting neutrophils. Without affecting the viability of resting cells, low concentrations of CpG ODN2006 interfered with Salmonella typhimurium-mediated viability prolongation and increased neutrophil apoptosis to control levels. CpG ODN2006 stimulated neutrophil apoptosis by enhancing ROS generation. Even small doses of ODN could induce the production of intracellular superoxide anions. The high superoxide reactogenicity, including with respect to nitrogen oxide, led to increased levels of intracellular ROS and RNS, which ultimately caused apoptosis. The pro-oxidant effect of low concentrations of CpG ODN2006 was not sufficient to trigger irreversible pro-apoptotic mechanisms. However, the sensitivity of PMNLs to ODN2006, a modulator of apoptosis, increased significantly und