Аннотация:
BACKGROUND: The spread of phytocomplex application and justification of its selective effects on tumour cells (mainly due to the presence of flavonoids) require research of its cytotoxic and immunomodulatory activity. AIM: The goal was to study the direct cytotoxic effect of the phytocomplex and its modulating effect on the cytotoxic activity of the donor’s mononuclear blood cells in in vitro experiments. METHODS: The phytocomplex was a dry extract from marsh cinquefoil, creeping alfalfa and common hop; its main active ingredients were flavonoids. Transplantable monolayer cultures of lung adenocarcinoma, colorectal cancer, erythroblastic leukaemia, and fibroblasts were used as target cells. The cytotoxic activity was assessed using a cytotoxic test based on the selective ability to live cells to reduce MTT (3-[4, 5-dimethyltriazol-2-yl]-2, 5 diphenyltetrazolium bromide) to formazan in mitochondria. Quantitative determination of formazan was performed using spectrophotometry. RESULTS: A
Ключевые слова:
Cytotoxic activity; Cytotoxic test; Flavonoids; Mononuclear blood cells; Plant extract
biochanin A; cytotoxic antibody; daidzein; flavonoid; genistein; interleukin 1; interleukin 2; isoflavone; quercetin; rutoside; alfalfa; Article; blood cell; blood transfusion; chemical structure; colorectal cancer; controlled study; cytotoxicity; erythroleukemia; fibroblast; human; human cell; immunomodulation; K-562 cell line; lung adenocarcinoma; medicinal plant; mitochondrion; mononuclear blood cell; MTT assay; nonhuman; optical density; phytochemistry; spectrophotometry
Babaskina L. I. Lyudmila Ivanovna 1952-
Litvinova T. M. Tat`yana Mikhaylovna 1962-
Babaskin D. V. Dmitrij Vladimirovich 1979-
Vinter E. A. Elizaveta Aleksandrovna 1973-
Kiselevsky M. V.
Savinova O. V. Ol`ga Vladimirovna 1970-
Бабаскина Л. И. Людмила Ивановна 1952-
Литвинова Т. М. Татьяна Михайловна 1962-
Бабаскин Д. В. Дмитрий Владимирович 1979-
Винтер Е. А. Елизавета Александровна 1973-
Киселевскy М. В.
Савинова О. В. Ольга Владимировна 1970-
Influence of flavonoids on the cytotoxic activity of mononuclear blood cells in model tests
Текст визуальный непосредственный
Open Access Macedonian Journal of Medical Sciences
Open Access Macedonian Journal of Medical Sciences
Vol.7, Issue12 P. 1900-1904
2019
Статья
Cytotoxic activity Cytotoxic test Flavonoids Mononuclear blood cells Plant extract
biochanin A cytotoxic antibody daidzein flavonoid genistein interleukin 1 interleukin 2 isoflavone quercetin rutoside alfalfa Article blood cell blood transfusion chemical structure colorectal cancer controlled study cytotoxicity erythroleukemia fibroblast human human cell immunomodulation K-562 cell line lung adenocarcinoma medicinal plant mitochondrion mononuclear blood cell MTT assay nonhuman optical density phytochemistry spectrophotometry
BACKGROUND: The spread of phytocomplex application and justification of its selective effects on tumour cells (mainly due to the presence of flavonoids) require research of its cytotoxic and immunomodulatory activity. AIM: The goal was to study the direct cytotoxic effect of the phytocomplex and its modulating effect on the cytotoxic activity of the donor’s mononuclear blood cells in in vitro experiments. METHODS: The phytocomplex was a dry extract from marsh cinquefoil, creeping alfalfa and common hop; its main active ingredients were flavonoids. Transplantable monolayer cultures of lung adenocarcinoma, colorectal cancer, erythroblastic leukaemia, and fibroblasts were used as target cells. The cytotoxic activity was assessed using a cytotoxic test based on the selective ability to live cells to reduce MTT (3-[4, 5-dimethyltriazol-2-yl]-2, 5 diphenyltetrazolium bromide) to formazan in mitochondria. Quantitative determination of formazan was performed using spectrophotometry. RESULTS: A