First application of atmospheric-pressure chemical ionization gas chromatography tandem mass spectrometry to the determination of cannabinoids in...
Gottardo R., Sorio D., Ballotari M., Tal`yaro F.
Journal of Chromatography A
Vol.1591, P. 147-154
Опубликовано: 2019
Тип ресурса: Статья
DOI:10.1016/j.chroma.2019.01.041
Аннотация:
The analysis of cannabinoids in blood samples is still a challenging issue for forensic laboratories, because of the low concentrations to be determined to prove that a person acted under CannabisTherefore, sensitive analytical techniques are required. This study presents the development and validation of a novel APGC-MS/MS method for the simultaneous determination of Δ9-tetrahydrocannabinol (THC), 11-hydroxy- Δ9-THC (THC−OH), 11-nor-9-carboxy- Δ9-THC (THCA), cannabidiol (CBD), cannabidiol acid (CBDA) and cannabigerol (CBG) in human serum. The developed method was fully validated according to international guidelines, with evaluation of selectivity, precision, accuracy, linearity, LODs and LOQs, extraction recovery and matrix effect. The method was linear in the range 0.2–25 ng/mL for THC, THC−OH, CBD and CBG, while for THCA and CBDA linearity was assessed in the range of 0.8–100 ng/mL and 3–100 ng/mL, respectively. The LOQs were determined in 0.2 ng/mL for THC, 0.4 ng/mL for THC−OH, 0
Ключевые слова:
Atmospheric pressure; Cannabinoids; Gas chromatography (AP GC); Gas chromatography (GC); Serum; Tandem mass spectrometry (MS/MS)
Atmospheric chemistry; Atmospheric ionization; Atmospheric pressure; Body fluids; Forensic science; Mass spectrometry; Atmospheric pressure chemical ionization; Cannabinoids; Extraction recovery; Forensic laboratory; Forensic toxicology; Serum; Simultaneous determinations; Tandem mass spectrometry; Gas chromatography; 11 hydroxydronabinol; cannabidiol; cannabidiol acid; cannabigerol; cannabinoid; dronabinol; tetrahydrocannabinolic acid; unclassified drug; cannabinoid; ion; accuracy; Article; atmospheric pressure chemical ionization mass spectrometry; blood sampling; gas chromatography; human; limit of detection; limit of quantitation; normal human; priority journal; process optimization; retention time; sensitivity analysis; tandem mass spectrometry; validation study; atmospheric pressure; blood; mass fragmentography; procedures; reproducibility; tandem mass spectrometry; Atmospheric Pressure; Cannabinoids; Gas Chromatography-Mass Spectrometry; Humans; Ions; Limit of Detection; Reprodu
Язык текста: Английский
ISSN: 1873-3778
Gottardo R.
Sorio D.
Ballotari M.
Tal`yaro F. Franko 1952-
Готтардо Р.
Сорио Д.
Баллотари М.
Тальяро Ф. Франко 1952-
First application of atmospheric-pressure chemical ionization gas chromatography tandem mass spectrometry to the determination of cannabinoids in serum
First application of atmospheric-pressure chemical ionization gas chromatography tandem mass spectrometry to the determination of cannabinoids in...
Текст визуальный непосредственный
Journal of Chromatography A
Elsevier Science Publisher B.V.
Vol.1591 P. 147-154
2019
Статья
Atmospheric pressure Cannabinoids Gas chromatography (AP GC) Gas chromatography (GC) Serum Tandem mass spectrometry (MS/MS)
Atmospheric chemistry Atmospheric ionization Atmospheric pressure Body fluids Forensic science Mass spectrometry Atmospheric pressure chemical ionization Cannabinoids Extraction recovery Forensic laboratory Forensic toxicology Serum Simultaneous determinations Tandem mass spectrometry Gas chromatography 11 hydroxydronabinol cannabidiol cannabidiol acid cannabigerol cannabinoid dronabinol tetrahydrocannabinolic acid unclassified drug cannabinoid ion accuracy Article atmospheric pressure chemical ionization mass spectrometry blood sampling gas chromatography human limit of detection limit of quantitation normal human priority journal process optimization retention time sensitivity analysis tandem mass spectrometry validation study atmospheric pressure blood mass fragmentography procedures reproducibility tandem mass spectrometry Atmospheric Pressure Cannabinoids Gas Chromatography-Mass Spectrometry Humans Ions Limit of Detection Reprodu
The analysis of cannabinoids in blood samples is still a challenging issue for forensic laboratories, because of the low concentrations to be determined to prove that a person acted under CannabisTherefore, sensitive analytical techniques are required. This study presents the development and validation of a novel APGC-MS/MS method for the simultaneous determination of Δ9-tetrahydrocannabinol (THC), 11-hydroxy- Δ9-THC (THC−OH), 11-nor-9-carboxy- Δ9-THC (THCA), cannabidiol (CBD), cannabidiol acid (CBDA) and cannabigerol (CBG) in human serum. The developed method was fully validated according to international guidelines, with evaluation of selectivity, precision, accuracy, linearity, LODs and LOQs, extraction recovery and matrix effect. The method was linear in the range 0.2–25 ng/mL for THC, THC−OH, CBD and CBG, while for THCA and CBDA linearity was assessed in the range of 0.8–100 ng/mL and 3–100 ng/mL, respectively. The LOQs were determined in 0.2 ng/mL for THC, 0.4 ng/mL for THC−OH, 0