Reflection-mode continuous-wave 0.15 λ -resolution terahertz solid immersion microscopy of soft biological tissues
Chernomy'rdin N. V., Kucheryavenko A. S., Kolontaeva G. S., Katyba G. M., Dolganova I. N., Karalkin P. A., Ponomarev D. S., Kurlov V. N., Reshetov I. V., Skorobogatiy M., Tuchin V. V., Zaytsev K. I.
Applied Physics Letters
Vol.113, Issue11, Num.111102
Опубликовано: 2018
Тип ресурса: Статья
Аннотация:
We have developed a method of terahertz (THz) solid immersion (SI) microscopy for continuous-wave reflection-mode imaging of soft biological tissues with a sub-wavelength spatial resolution. In order to achieve strong reduction in the dimensions of the THz beam caustic, an electromagnetic wave is focused into the evanescent field volume behind a medium with a high refractive index. We have experimentally demonstrated a 0.15λ-resolution of the proposed imaging modality at λ = 500 μm, which is beyond the Abbe diffraction limit and represents a considerable improvement over the previously-reported arrangements of SI imaging setups. The proposed technique does not involve any sub-wavelength near-field probes and diaphragms, thus, avoiding the THz beam attenuation due to such elements. We have applied the developed method for THz imaging of various soft tissues: a plant leaf blade, cell spheroids, and tissues of the breast ex vivo. Our THz images clearly reveal sub-wavelength features in ti
Ключевые слова:
Diffraction; Electromagnetic waves; Evanescent fields; Histology; Medical imaging; Plants (botany); Refractive index; Terahertz waves; Tissue engineering; Abbe diffraction; Biology and medicine; High refractive index; Near field probes; Reflection modes; Soft biological tissue; Solid immersion microscopy; Spatial resolution; Tissue
Язык текста: Английский
ISSN: 1077-3118
Chernomy'rdin N. V. Nikita Viktorovich 1992-
Kucheryavenko A. S.
Kolontaeva G. S.
Katyba G. M.
Dolganova I. N. Irina Nikolaevna 1988-
Karalkin P. A.
Ponomarev D. S.
Kurlov V. N. Vladimir Nikolaevich 1959-
Reshetov I. V. Igor` Vladimirovich 1964-
Skorobogatiy M.
Tuchin V. V.
Zaytsev K. I. Kirill Igorevich 1989-
Черномырдин Н. В. Никита Викторович 1992-
Кучерявенко А. С.
Колонтаева Г. С.
Катyба Г. М.
Долганова И. Н. Ирина Николаевна 1988-
Каралкин П. А.
Пономарев Д. С.
Курлов В. Н. Владимир Николаевич 1959-
Решетов И. В. Игорь Владимирович 1964-
Скоробогатиy М.
Тючин В. В.
Зайцев К. И. Кирилл Игоревич 1989-
Reflection-mode continuous-wave 0.15 λ -resolution terahertz solid immersion microscopy of soft biological tissues
Текст визуальный непосредственный
Applied Physics Letters
American Institute of Physics
Vol.113, Issue11 Num.111102
2018
Статья
Diffraction Electromagnetic waves Evanescent fields Histology Medical imaging Plants (botany) Refractive index Terahertz waves Tissue engineering Abbe diffraction Biology and medicine High refractive index Near field probes Reflection modes Soft biological tissue Solid immersion microscopy Spatial resolution Tissue
We have developed a method of terahertz (THz) solid immersion (SI) microscopy for continuous-wave reflection-mode imaging of soft biological tissues with a sub-wavelength spatial resolution. In order to achieve strong reduction in the dimensions of the THz beam caustic, an electromagnetic wave is focused into the evanescent field volume behind a medium with a high refractive index. We have experimentally demonstrated a 0.15λ-resolution of the proposed imaging modality at λ = 500 μm, which is beyond the Abbe diffraction limit and represents a considerable improvement over the previously-reported arrangements of SI imaging setups. The proposed technique does not involve any sub-wavelength near-field probes and diaphragms, thus, avoiding the THz beam attenuation due to such elements. We have applied the developed method for THz imaging of various soft tissues: a plant leaf blade, cell spheroids, and tissues of the breast ex vivo. Our THz images clearly reveal sub-wavelength features in ti