The encapsulation of lycopene in nanoliposomes enhances its protective potential in methotrexate-induced kidney injury model
Stojiljkovic N., Ilic S., Yakovlevich V., Stojanovic N., Stojnev S., Kocic H., Stojanovic M., Kocic G.
Oxidative Medicine and Cellular Longevity
Vol.2018, Num.2627917
Опубликовано: 2018
Тип ресурса: Статья
Аннотация:
Methotrexate is an antimetabolic drug with a myriad of serious side effects including nephrotoxicity, which presumably occurs due to oxidative tissue damage. Here, we evaluated the potential protective effect of lycopene, a potent antioxidant carotenoid, given in two different pharmaceutical forms in methotrexate-induced kidney damage in rats. Serum biochemical (urea and creatinine) and tissue oxidative damage markers and histopathological kidney changes were evaluated after systemic administration of both lycopene dissolved in corn oil and lycopene encapsulated in nanoliposomes. Similar to previous studies, single dose of methotrexate induced severe functional and morphological alterations of kidneys with cell desquamation, tubular vacuolation, and focal necrosis, which were followed by serum urea and creatinine increase and disturbances of tissue antioxidant status. Application of both forms of lycopene concomitantly with methotrexate ameliorated changes in serum urea and creatinine
Ключевые слова:
catalase; creatinine; liposome; lycopene; malonaldehyde; methotrexate; myeloperoxidase; nanocarrier; nanoliposome; unclassified drug; urea; carotenoid; lycopene; methotrexate; animal experiment; animal model; animal tissue; antioxidant activity; Article; cell vacuole; controlled study; creatinine blood level; drug stability; enzyme activity; histopathology; in vitro study; kidney injury; kidney tissue; lipid peroxidation; male; nanoencapsulation; nephrotoxicity; nonhuman; pH; protein determination; rat; renal protection; sustained drug release; urea blood level; animal; chemically induced; kidney; kidney disease; metabolism; pathology; Wistar rat; Animals; Carotenoids; Kidney; Kidney Diseases; Male; Methotrexate; Rats; Rats, Wistar
Язык текста: Английский
ISSN: 1942-0994
Stojiljkovic N.
Ilic S.
Yakovlevich V. Vladimir 1971-
Stojanovic N.
Stojnev S.
Kocic H.
Stojanovic M.
Kocic G.
Стойилйковиc Н.
Илиc С.
Яковлевич В. Владимир 1971-
Стойановиc Н.
Стойнев С.
Коcиc Х.
Стойановиc М.
Коcиc Г.
The encapsulation of lycopene in nanoliposomes enhances its protective potential in methotrexate-induced kidney injury model
Текст визуальный непосредственный
Oxidative Medicine and Cellular Longevity
Vol.2018 Num.2627917
2018
Статья
catalase creatinine liposome lycopene malonaldehyde methotrexate myeloperoxidase nanocarrier nanoliposome unclassified drug urea carotenoid lycopene methotrexate animal experiment animal model animal tissue antioxidant activity Article cell vacuole controlled study creatinine blood level drug stability enzyme activity histopathology in vitro study kidney injury kidney tissue lipid peroxidation male nanoencapsulation nephrotoxicity nonhuman pH protein determination rat renal protection sustained drug release urea blood level animal chemically induced kidney kidney disease metabolism pathology Wistar rat Animals Carotenoids Kidney Kidney Diseases Male Methotrexate Rats Rats, Wistar
Methotrexate is an antimetabolic drug with a myriad of serious side effects including nephrotoxicity, which presumably occurs due to oxidative tissue damage. Here, we evaluated the potential protective effect of lycopene, a potent antioxidant carotenoid, given in two different pharmaceutical forms in methotrexate-induced kidney damage in rats. Serum biochemical (urea and creatinine) and tissue oxidative damage markers and histopathological kidney changes were evaluated after systemic administration of both lycopene dissolved in corn oil and lycopene encapsulated in nanoliposomes. Similar to previous studies, single dose of methotrexate induced severe functional and morphological alterations of kidneys with cell desquamation, tubular vacuolation, and focal necrosis, which were followed by serum urea and creatinine increase and disturbances of tissue antioxidant status. Application of both forms of lycopene concomitantly with methotrexate ameliorated changes in serum urea and creatinine