Thanatochemistry at the crime scene: a microfluidic paper-based device for ammonium analysis in the vitreous humor
Musile D., Agard Y., De P. E. F., Shestakova K. M., Bortolotti F., Tal`yaro F.
Analytica Chimica Acta
Vol.1083, P. 150-156
Опубликовано: 2019
Тип ресурса: Статья
DOI:10.1016/j.aca.2019.07.033
Аннотация:
Most of the on-site approaches for inferring of the post-mortem interval are still based on observative data from the direct body inspection, whereas, objective and quantitative analyses, such as potassium in the vitreous humor, are require laboratory instrumentation and skilled personnel. The present paper presents a simple and low cost analytical method suitable for use at the crime scene for inferring the time since death. The method uses a microfluidic paper-based device (μPAD) for the determination of ammonium in the vitreous humor (VH) based on the selective interaction between the ammonium and the Nessler's reagent. The color change was measured in terms of “RGB distance” by using a simple and free smartphone application. The optimized device showed a limit of detection of 0.4 mmol L−1, with between days precision less than 9.3[%] expressed as relative standard deviation, and accuracy between days from 94.5[%] to 104.5[%]. The selectivity of the Nessler's reaction was tested towards t
Ключевые слова:
Ammonium; Microfluidic paper-based devices (μPADs); Post-mortem interval; Thanatochemistry at the crime scene; Vitreous humor
Analytic equipment; Capillary electrophoresis; Microfluidics; Ammonium; Crime scenes; Paper based devices; Post mortem intervals; Vitreous humor; Crime; ammonia; ammonium derivative; iodide; mercury derivative; potassium tetraiodomercurate; adult; aged; Article; autopsy; capillary electrophoresis; controlled study; crime; human; human tissue; instrument validation; limit of detection; limit of quantitation; measurement accuracy; measurement precision; microtechnology; mobile application; priority journal; process optimization; reproducibility; vitreous body; chemistry; colorimetry; devices; forensic medicine; microfluidic analysis; middle aged; paper; postmortem change; procedures; proof of concept; time factor; vitreous body; young adult; Adult; Aged; Ammonium Compounds; Colorimetry; Forensic Medicine; Humans; Iodides; Limit of Detection; Mercury Compounds; Microfluidic Analytical Techniques; Middle Aged; Paper; Postmortem Changes; Proof of Concept Study; Reproducibility of Results; T
Язык текста: Английский
ISSN: 1873-4324
Musile D. Dzhakomo 1985-
Agard Y.
De P. E. F. Palo E.F.
Shestakova K. M. Kseniya Mikhaylovna 1992-
Bortolotti F.
Tal`yaro F. Franko 1952-
Мусиле Д. Джакомо 1985-
Агард Y.
Де П. Е. Ф. Пало Е.Ф.
Шестакова К. М. Ксения Михайловна 1992-
Бортолотти Ф.
Тальяро Ф. Франко 1952-
Thanatochemistry at the crime scene: a microfluidic paper-based device for ammonium analysis in the vitreous humor
Текст визуальный непосредственный
Analytica Chimica Acta
Elsevier Science Publisher B.V.
Vol.1083 P. 150-156
2019
Статья
Ammonium Microfluidic paper-based devices (μPADs) Post-mortem interval Thanatochemistry at the crime scene Vitreous humor
Analytic equipment Capillary electrophoresis Microfluidics Ammonium Crime scenes Paper based devices Post mortem intervals Vitreous humor Crime ammonia ammonium derivative iodide mercury derivative potassium tetraiodomercurate adult aged Article autopsy capillary electrophoresis controlled study crime human human tissue instrument validation limit of detection limit of quantitation measurement accuracy measurement precision microtechnology mobile application priority journal process optimization reproducibility vitreous body chemistry colorimetry devices forensic medicine microfluidic analysis middle aged paper postmortem change procedures proof of concept time factor vitreous body young adult Adult Aged Ammonium Compounds Colorimetry Forensic Medicine Humans Iodides Limit of Detection Mercury Compounds Microfluidic Analytical Techniques Middle Aged Paper Postmortem Changes Proof of Concept Study Reproducibility of Results
Most of the on-site approaches for inferring of the post-mortem interval are still based on observative data from the direct body inspection, whereas, objective and quantitative analyses, such as potassium in the vitreous humor, are require laboratory instrumentation and skilled personnel. The present paper presents a simple and low cost analytical method suitable for use at the crime scene for inferring the time since death. The method uses a microfluidic paper-based device (μPAD) for the determination of ammonium in the vitreous humor (VH) based on the selective interaction between the ammonium and the Nessler's reagent. The color change was measured in terms of “RGB distance” by using a simple and free smartphone application. The optimized device showed a limit of detection of 0.4 mmol L−1, with between days precision less than 9.3[%] expressed as relative standard deviation, and accuracy between days from 94.5[%] to 104.5[%]. The selectivity of the Nessler's reaction was tested towards t