Modulating cancer cell mechanics and actin cytoskeleton structure by chemical and mechanical stimulations
Azadi S., Tafazzoli-Shadpour M., Soleimani M., Ibragimi V. M. M.
Journal of Biomedical Materials Research - Part A
Vol.107, Issue8, P. 1569-1581
Опубликовано: 2019
Тип ресурса: Статья
Аннотация:
To date, a myriad of strategies has been suggested for targeting the chemical signaling of cancer cells. Also, biomechanical features are gaining much more attention. These features can be used as biomarkers which influence cancer progression. Current approaches on cancer treatment are mainly focused on changing the biochemical signaling of cancer cells, whereas less attention was devoted to their biomechanical properties. Herein, we propose targeting of cancer cell mechanics through the microenvironmental mechanical and chemical cues. As such, we examined the role of substrate stiffness as well as the effect of epidermal growth factor receptor (EGFR) blockade in the cell mechanics. As a mechanical stimulus, stiff and soft polydimethylsiloxane substrates were utilized, while as a chemical stimulus, EGFR blockade was considered. Thus, breast cancer cell lines, MCF7 and MDA-MB-231, were cultured among chemical and mechanical groups. The local elasticity of cancer cells was assessed by at
Ключевые слова:
actin cytoskeleton; atomic force microscopy; EGFR; substrate stiffness; Young's modulus
Atomic force microscopy; Biomechanics; Cell culture; Cells; Diseases; Elastic moduli; Molecular biology; Morphology; Proteins; Silicones; Stiffness; Substrates; Actin cytoskeleton; Biomechanical properties; EGFR; Epidermal growth factor receptors; Mechanical stimulation; Nano-indentation methods; Substrate stiffness; Tumor cell migration; Cell signaling; cetuximab; dimeticone; epidermal growth factor receptor; epidermal growth factor receptor kinase inhibitor; baysilon; dimeticone; actin filament; Article; atomic force microscopy; breast cancer cell line; cancer cell culture; cell migration; cell structure; chemical stimulation; controlled study; elasticity; human; human cell; mechanical stimulation; signal transduction; tumor invasion; Young modulus; actin filament; cell death; cell motion; cell shape; drug effect; mechanical stress; metabolism; neoplasm; pathology; tumor cell line; Actin Cytoskeleton; Cell Death; Cell Line, Tumor; Cell Movement; Cell Shape; Dimethylpolysiloxanes; Ela
Язык текста: Английский
ISSN: 1552-4965
Azadi S.
Tafazzoli-Shadpour M.
Soleimani M.
Ibragimi V. M. M. Varkiani Madzhid Modzhtaba 1983-
Азади С.
Тафаззоли-Шадпоур М.
Солеимани М.
Ибрагими В. М. М. Варкиани Маджид Моджтаба 1983-
Modulating cancer cell mechanics and actin cytoskeleton structure by chemical and mechanical stimulations
Текст визуальный непосредственный
Journal of Biomedical Materials Research - Part A
John Wiley & Sons
Vol.107, Issue8 P. 1569-1581
2019
Статья
actin cytoskeleton atomic force microscopy EGFR substrate stiffness Young's modulus
Atomic force microscopy Biomechanics Cell culture Cells Diseases Elastic moduli Molecular biology Morphology Proteins Silicones Stiffness Substrates Actin cytoskeleton Biomechanical properties EGFR Epidermal growth factor receptors Mechanical stimulation Nano-indentation methods Substrate stiffness Tumor cell migration Cell signaling cetuximab dimeticone epidermal growth factor receptor epidermal growth factor receptor kinase inhibitor baysilon dimeticone actin filament Article atomic force microscopy breast cancer cell line cancer cell culture cell migration cell structure chemical stimulation controlled study elasticity human human cell mechanical stimulation signal transduction tumor invasion Young modulus actin filament cell death cell motion cell shape drug effect mechanical stress metabolism neoplasm pathology tumor cell line Actin Cytoskeleton Cell Death Cell Line, Tumor Cell Movement Cell Shape Dimethylpolysiloxanes Ela
To date, a myriad of strategies has been suggested for targeting the chemical signaling of cancer cells. Also, biomechanical features are gaining much more attention. These features can be used as biomarkers which influence cancer progression. Current approaches on cancer treatment are mainly focused on changing the biochemical signaling of cancer cells, whereas less attention was devoted to their biomechanical properties. Herein, we propose targeting of cancer cell mechanics through the microenvironmental mechanical and chemical cues. As such, we examined the role of substrate stiffness as well as the effect of epidermal growth factor receptor (EGFR) blockade in the cell mechanics. As a mechanical stimulus, stiff and soft polydimethylsiloxane substrates were utilized, while as a chemical stimulus, EGFR blockade was considered. Thus, breast cancer cell lines, MCF7 and MDA-MB-231, were cultured among chemical and mechanical groups. The local elasticity of cancer cells was assessed by at