Biofabrication of tissue-specific extracellular matrix proteins to enhance the expansion and differentiation of skeletal muscle progenitor cells
Li L., Liu G., Timashev P. S., Sun X. S., Criswell T., Atala A., Zhang Y.
Applied Physics Reviews
Vol.6, Issue2, Num.021309
Опубликовано: 2019
Тип ресурса: Статья
Аннотация:
Skeletal muscle precursor cells (MPCs) are considered key candidates for cell therapy in the treatment of skeletal muscle dysfunction due to injury, disease, or aging. However, expansion of a sufficient number of functional skeletal muscle cells in vitro from a small tissue biopsy has been challenging due to changes in the phenotypic expression of these cells under nonnatural microenvironmental or traditional culture conditions. This review provides an overview of recent progress in the design and biofabrication of advanced tissue-specific extracellular matrix (ECM) proteins for use in the enhancement of expansion and differentiation of MPCs for cell therapy and 3D bioprinting. We start with a brief introduction about the existing progress, drawbacks, and emerging challenges in the culture and maintenance of long term primary human MPCs for cell therapy. With regard to MPC proliferation, elongation, fusion, and differentiation into mature myofibers, we systematically summarize the bene
Ключевые слова:
Cells; Cytology; Disease control; Muscle; Proteins; Extracellular matrix protein; Phenotypic expression; Recent progress; Skeletal muscle; Skeletal muscle cells; Tissue biopsies; Tissue specifics; Traditional cultures; Tissue regeneration
Язык текста: Английский
ISSN: 1931-9401
Li L.
Liu G.
Timashev P. S. Petr Sergeevich 1978-
Sun X. S.
Criswell T.
Atala A.
Zhang Y.
Ли Л.
Лиу Г.
Тимашев П. С. Петр Сергеевич 1978-
Сун Х. С.
Cрисwелл Т.
Атала А.
Жанг Y.
Biofabrication of tissue-specific extracellular matrix proteins to enhance the expansion and differentiation of skeletal muscle progenitor cells
Текст визуальный непосредственный
Applied Physics Reviews
American Institute of Physics Inc.
Vol.6, Issue2 Num.021309
2019
Статья
Cells Cytology Disease control Muscle Proteins Extracellular matrix protein Phenotypic expression Recent progress Skeletal muscle Skeletal muscle cells Tissue biopsies Tissue specifics Traditional cultures Tissue regeneration
Skeletal muscle precursor cells (MPCs) are considered key candidates for cell therapy in the treatment of skeletal muscle dysfunction due to injury, disease, or aging. However, expansion of a sufficient number of functional skeletal muscle cells in vitro from a small tissue biopsy has been challenging due to changes in the phenotypic expression of these cells under nonnatural microenvironmental or traditional culture conditions. This review provides an overview of recent progress in the design and biofabrication of advanced tissue-specific extracellular matrix (ECM) proteins for use in the enhancement of expansion and differentiation of MPCs for cell therapy and 3D bioprinting. We start with a brief introduction about the existing progress, drawbacks, and emerging challenges in the culture and maintenance of long term primary human MPCs for cell therapy. With regard to MPC proliferation, elongation, fusion, and differentiation into mature myofibers, we systematically summarize the bene