Detection of Low-Abundant MicroRNAs with Hybridization Microchips
Shkurnikov M. Y., Knyazev E. N., Fomicheva K. A., Kasatkin M. S., Arkov V. V., Makarova Y. A.
Bulletin of Experimental Biology and Medicine
Vol.166, Issue6, P. 788-792
Опубликовано: 2019
Тип ресурса: Статья
DOI:10.1007/s10517-019-04441-z
Аннотация:
The effect of low concentrations of miRNA on the ability of GeneChip miRNA 4.0 hybridization chips to evaluate their representation in the sample was studied. It is shown that the evaluation of the expression of 61 miRNAs is statistically significantly associated with the multiplicity of plasma dilution. Only 12 miRNAs showed very high Pearson correlation coefficient (>0.95) and they all decreased in response to dilution. High abundance of has-miR-4532 miRNA in plasma was demonstrated. This miRNA was never detected during sequencing of similar samples. It was concluded that in case of miRNA expression <1.12±0.33 units in log2 scale, dilution was not followed by further decrease in the signal intensity in GeneChip miRNA 4.0 chips. © 2019, Springer Science+Business Media, LLC, part of Springer Nature.
Ключевые слова:
hybridization chips; miRNA; sensitivity
has mir 3619 5p; has mir 3921; has mir 6732 5p; hsa let 7b 5p; hsa mir 106a 5p; hsa mir 16 5p; hsa mir 185 5p; hsa mir 19b 3p; hsa mir 22 3p; hsa mir 320e; hsa mir 4454; hsa mir 4532; hsa mir 486 5p; hsa mir 619 5p; microRNA; unclassified drug; microRNA; Article; correlation analysis; correlation coefficient; DNA hybridization; hsa mir 92a 3p; human; human cell; microchip analysis; RNA isolation; room temperature; blood; genetics; microarray analysis; nucleic acid hybridization; procedures; sensitivity and specificity; specimen handling; Humans; Microarray Analysis; MicroRNAs; Nucleic Acid Hybridization; Sensitivity and Specificity; Specimen Handling
Язык текста: Английский
ISSN: 1573-8221
Shkurnikov M. Y.
Knyazev E. N.
Fomicheva K. A.
Kasatkin M. S. Mikhail Sergeevich 1987-
Arkov V. V.
Makarova Y. A.
Шкурников М. Y.
Князев Е. Н.
Фомичева К. А.
Касаткин М. С. Михаил Сергеевич 1987-
Арков В. В.
Макарова Y. А.
Detection of Low-Abundant MicroRNAs with Hybridization Microchips
Текст визуальный непосредственный
Bulletin of Experimental Biology and Medicine
Springer New York Consultants Bureau
Vol.166, Issue6 P. 788-792
2019
Статья
hybridization chips miRNA sensitivity
has mir 3619 5p has mir 3921 has mir 6732 5p hsa let 7b 5p hsa mir 106a 5p hsa mir 16 5p hsa mir 185 5p hsa mir 19b 3p hsa mir 22 3p hsa mir 320e hsa mir 4454 hsa mir 4532 hsa mir 486 5p hsa mir 619 5p microRNA unclassified drug microRNA Article correlation analysis correlation coefficient DNA hybridization hsa mir 92a 3p human human cell microchip analysis RNA isolation room temperature blood genetics microarray analysis nucleic acid hybridization procedures sensitivity and specificity specimen handling Humans Microarray Analysis MicroRNAs Nucleic Acid Hybridization Sensitivity and Specificity Specimen Handling
The effect of low concentrations of miRNA on the ability of GeneChip miRNA 4.0 hybridization chips to evaluate their representation in the sample was studied. It is shown that the evaluation of the expression of 61 miRNAs is statistically significantly associated with the multiplicity of plasma dilution. Only 12 miRNAs showed very high Pearson correlation coefficient (>0.95) and they all decreased in response to dilution. High abundance of has-miR-4532 miRNA in plasma was demonstrated. This miRNA was never detected during sequencing of similar samples. It was concluded that in case of miRNA expression <1.12±0.33 units in log2 scale, dilution was not followed by further decrease in the signal intensity in GeneChip miRNA 4.0 chips. © 2019, Springer Science+Business Media, LLC, part of Springer Nature.