Effect of various agents on the direction of THP-1 cell differentiation [Uticaj različitih agenasa na SMER THP-1 ćelijske diferencijacije]
Zubova S. V., Radzyukevich Y. V., Grachev S. V., Prokhorenko I. R.
Serbian Journal of Experimental and Clinical Research
Vol.19, Issue3, P. 263-269
Опубликовано: 2018
Тип ресурса: Статья
DOI:10.2478/SJECR-2018-0029
Аннотация:
The ability of physiological (1α,25-dihydroxyvitamin D3, retinoic acid) and non-physiological (various LPS) agents and their combinations to influence the direction of pro-monocytic THP-1 cell differentiation was studied. The differentiating activity of the agents was evaluated by the expression and the ratio of surface receptors (TLR4, CD11b, and CD14) as well as by the change in THP-1 cell phagocytic activity of different degree of differentiation by Flow cytometry. The THP-1 cell differentiation by VD3 was shown to lead probably to the formation of classical monocytes. Summarizing we can conclude that VD3 induces the THP-1 cells differentiation with the formation of classical monocytes and the sequence of 1α, 25-dihydroxyvitamin D3 and non-toxic LPS R. capsulatus PG causes the THP-1 cells differentiation with the formation of inflammatory or intermediate monocytes. © 2018, University of Kragujevac, Faculty of Science. All rights reserved.
Ключевые слова:
Lipopolysaccharides; Phagocytosis; Receptor expression; Retinoic acid; Vitamin D3; ТНР-1 cells
calcitriol; CD11b antigen; CD14 antigen; retinoic acid; toll like receptor 4; Article; bacterial strain; cell differentiation; controlled study; Escherichia coli; flow cytometry; fluorescence; human; human cell; nonhuman; phagocytosis; protein expression; Rhodobacter capsulatus; Salmonella enterica serovar Typhimurium; THP-1 cell line
Язык текста: Английский
ISSN: 1820-8665
Zubova S. V.
Radzyukevich Y. V.
Grachev S. V. Sergej Vitalyevich 1948-
Prokhorenko I. R.
Зубова С. В.
Радзюкевич Y. В.
Грачев С. В. Сергей Витальевич 1948-
Прохоренко И. Р.
Effect of various agents on the direction of THP-1 cell differentiation [Uticaj različitih agenasa na SMER THP-1 ćelijske diferencijacije]
Текст визуальный непосредственный
Serbian Journal of Experimental and Clinical Research
Vol.19, Issue3 P. 263-269
2018
Статья
Lipopolysaccharides Phagocytosis Receptor expression Retinoic acid Vitamin D3 ТНР-1 cells
calcitriol CD11b antigen CD14 antigen retinoic acid toll like receptor 4 Article bacterial strain cell differentiation controlled study Escherichia coli flow cytometry fluorescence human human cell nonhuman phagocytosis protein expression Rhodobacter capsulatus Salmonella enterica serovar Typhimurium THP-1 cell line
The ability of physiological (1α,25-dihydroxyvitamin D3, retinoic acid) and non-physiological (various LPS) agents and their combinations to influence the direction of pro-monocytic THP-1 cell differentiation was studied. The differentiating activity of the agents was evaluated by the expression and the ratio of surface receptors (TLR4, CD11b, and CD14) as well as by the change in THP-1 cell phagocytic activity of different degree of differentiation by Flow cytometry. The THP-1 cell differentiation by VD3 was shown to lead probably to the formation of classical monocytes. Summarizing we can conclude that VD3 induces the THP-1 cells differentiation with the formation of classical monocytes and the sequence of 1α, 25-dihydroxyvitamin D3 and non-toxic LPS R. capsulatus PG causes the THP-1 cells differentiation with the formation of inflammatory or intermediate monocytes. © 2018, University of Kragujevac, Faculty of Science. All rights reserved.