Structural, immunohistochemical and molecular features of placentas and placental sites after in vitro fertilization with donor eggs (surrogate...

Коган Е. А., Руденко Е. Е., Демура Т. А., Zharkov N. V., Trifonova N. S., Zhukova E. V., Aleksandrov L. S., Bayanova S. N.

European Journal of Obstetrics & Gynecology and Reproductive Biology

Vol.238, P. 68-72

Опубликовано: 2019

Тип ресурса: Статья

DOI:10.1016/j.ejogrb.2019.05.006

Аннотация:

Objective: to identify structural, immunohistochemical and molecular features of placentas and placental sites afterin vitro fertilization (IVF) with donor eggs (surrogate motherhood). Study design: morphological and immunohistochemical studies were performed on placental material obtained after delivery by caesarean section. The study included 26 women patients whose pregnancy resulted from IVF with a donor egg (IVF-SM group). The comparison group included 13 women patients whose pregnancy occurred after IVF with their own eggs (IVF-OE). Immunohistochemistry of biopsy material was performed using mouse antibodies to total cytokeratin (clone AE1/AE3) and murine antibodies to HLA-DR (clone TAL.1B5). Molecular studies were performed on DNA samples isolated from venous blood. HLA-DNA-TEH reagent kits and polymerase chain reaction were used for genotyping the main human histocompatibility complex class II (DQA1, DQB1 and DRB1). Results: Histological examination of placenta in IVF-SM group

Ключевые слова:

HLA-DR; Immune alteration; Immunological tolerance; Placental site; Surrogacy

fibrin; HLA antigen class 2; Article; chorion villus; clinical article; controlled study; female; genetic polymorphism; human; human tissue; immunohistochemistry; in vitro fertilization; infarction; inflammation; ischemia; molecular biology; morphology; multinuclear cell; oocyte donor; placenta development; priority journal; surrogate mother; case control study; immunology; in vitro fertilization; pathology; placenta; pregnancy; Case-Control Studies; Female; Fertilization in Vitro; Humans; Placenta; Placentation; Pregnancy; Surrogate Mothers

Язык текста: Английский

ISSN: 1872-7654

Коган Е. А. Евгения Алтаровна 1951-

Руденко Е. Е. Екатерина Евгеньевна 1988-

Демура Т. А. Татьяна Александровна 1983-

Zharkov N. V. Nikolay Vladimirovich 1975-

Trifonova N. S. Natal`ya Syaitovna 1979-

Zhukova E. V. Ekaterina Vladimirovna 1987-

Aleksandrov L. S. Leonid Semenovich 1963-

Bayanova S. N. Sof`ya Nikolaevna 1997-

Kogan E. A. Evgeniya Altarovna 1951-

Rudenko E. E. Ekaterina Evgenyevna 1988-

Demura T. A. Tat`yana Aleksandrovna 1983-

Жарков Н. В. Николай Владимирович 1975-

Трифонова Н. С. Наталья Сяитовна 1979-

Жукова Е. В. Екатерина Владимировна 1987-

Александров Л. С. Леонид Семенович 1963-

Баянова С. Н. Софья Николаевна 1997-

Structural, immunohistochemical and molecular features of placentas and placental sites after in vitro fertilization with donor eggs (surrogate motherhood)

Structural, immunohistochemical and molecular features of placentas and placental sites after in vitro fertilization with donor eggs (surrogate...

Текст визуальный непосредственный

European Journal of Obstetrics & Gynecology and Reproductive Biology

Elsevier Science Publisher B.V.

Vol.238 P. 68-72

2019

Статья

HLA-DR Immune alteration Immunological tolerance Placental site Surrogacy

fibrin HLA antigen class 2 Article chorion villus clinical article controlled study female genetic polymorphism human human tissue immunohistochemistry in vitro fertilization infarction inflammation ischemia molecular biology morphology multinuclear cell oocyte donor placenta development priority journal surrogate mother case control study immunology in vitro fertilization pathology placenta pregnancy Case-Control Studies Female Fertilization in Vitro Humans Placenta Placentation Pregnancy Surrogate Mothers

Objective: to identify structural, immunohistochemical and molecular features of placentas and placental sites afterin vitro fertilization (IVF) with donor eggs (surrogate motherhood). Study design: morphological and immunohistochemical studies were performed on placental material obtained after delivery by caesarean section. The study included 26 women patients whose pregnancy resulted from IVF with a donor egg (IVF-SM group). The comparison group included 13 women patients whose pregnancy occurred after IVF with their own eggs (IVF-OE). Immunohistochemistry of biopsy material was performed using mouse antibodies to total cytokeratin (clone AE1/AE3) and murine antibodies to HLA-DR (clone TAL.1B5). Molecular studies were performed on DNA samples isolated from venous blood. HLA-DNA-TEH reagent kits and polymerase chain reaction were used for genotyping the main human histocompatibility complex class II (DQA1, DQB1 and DRB1). Results: Histological examination of placenta in IVF-SM group