Time course of transient expression of pre-α-pro-GDNF and pre-β-pro-GDNF transcripts, and mGDNF mRNA region in Krushinsky–Molodkina rat brain after...
Solius G., Panteleev D., Pustogarov N., Revishchin A., Poletaeva I., Pavlova G. V.
Epilepsy & Behavior
Vol.96, P. 87-91
Опубликовано: 2019
Тип ресурса: Статья
DOI:10.1016/j.yebeh.2019.03.007
Аннотация:
The expression of glial cell line-derived neurothrophic factor (GDNF) transcript forms pre-(α)pro-gdnf, pre-(β)pro-gdnf, and their common region m-gdnf in the pons as well as the inferior (IC) and superior colliculi in Krushinsky–Molodkina (KM) rats and in the strain “0” was analyzed by quantitative real-time polymerase chain reaction (PCR) in the control (unstimulated KM and “0” rats) and 1.5, 4.5, and 8 h after auditory stimulation. Such stimulation induced audiogenic seizures (AS) in KM rats. Audiogenic seizure was not observed in “0” rats, which was obtained by selection for the absence of AS in a population of F2 hybrids between KM and Wistar rats not predisposed to AS. A significant drop in the level of all transcripts was observed 1.5 h after auditory stimulation in both KM and “0” rats. In most cases, the average expression of α and β isoforms and m-region 4.5 h after stimulation was greater than those after 1.5 and 8 h. At the same time, the expression of pre-(β)pro-gdnf in th
Ключевые слова:
Audiogenic seizures; Brainstem; GDNF transcripts; Rat brain
glial cell line derived neurotrophic factor; messenger RNA; Gdnf protein, rat; glial cell line derived neurotrophic factor; isoprotein; messenger RNA; animal experiment; animal model; animal tissue; Article; audiogenic seizure; auditory stimulation; controlled study; gene; gene expression; genetic transcription; genotype; inferior colliculus; Krushinsky Molodkina rat; m gdnf gene; nonhuman; pons; pre (alpha)pro gdnf gene; pre (beta)pro gdnf gene; rat; rat strain; real time polymerase chain reaction; superior colliculus; time; animal; biosynthesis; brain; gene expression; genetics; glia; male; metabolism; pathology; procedures; reflex epilepsy; seizure; time factor; Wistar rat; Acoustic Stimulation; Animals; Brain; Epilepsy, Reflex; Gene Expression; Glial Cell Line-Derived Neurotrophic Factor; Male; Neuroglia; Protein Isoforms; Rats; Rats, Wistar; RNA, Messenger; Seizures; Time Factors
Язык текста: Английский
ISSN: 1525-5069
Solius G.
Panteleev D.
Pustogarov N.
Revishchin A.
Poletaeva I.
Pavlova G. V. Galina Valerievna 1967-
Солиус Г.
Пантелеев Д.
Пустогаров Н.
Ревишчин А.
Полетаева И.
Павлова Г. В. Галина Валериевна 1967-
Time course of transient expression of pre-α-pro-GDNF and pre-β-pro-GDNF transcripts, and mGDNF mRNA region in Krushinsky–Molodkina rat brain after audiogenic seizures
Time course of transient expression of pre-α-pro-GDNF and pre-β-pro-GDNF transcripts, and mGDNF mRNA region in Krushinsky–Molodkina rat brain after...
Текст визуальный непосредственный
Epilepsy & Behavior
Academic Press
Vol.96 P. 87-91
2019
Статья
Audiogenic seizures Brainstem GDNF transcripts Rat brain
glial cell line derived neurotrophic factor messenger RNA Gdnf protein, rat glial cell line derived neurotrophic factor isoprotein messenger RNA animal experiment animal model animal tissue Article audiogenic seizure auditory stimulation controlled study gene gene expression genetic transcription genotype inferior colliculus Krushinsky Molodkina rat m gdnf gene nonhuman pons pre (alpha)pro gdnf gene pre (beta)pro gdnf gene rat rat strain real time polymerase chain reaction superior colliculus time animal biosynthesis brain gene expression genetics glia male metabolism pathology procedures reflex epilepsy seizure time factor Wistar rat Acoustic Stimulation Animals Brain Epilepsy, Reflex Gene Expression Glial Cell Line-Derived Neurotrophic Factor Male Neuroglia Protein Isoforms Rats Rats, Wistar RNA, Messenger Seizures Time Factors
The expression of glial cell line-derived neurothrophic factor (GDNF) transcript forms pre-(α)pro-gdnf, pre-(β)pro-gdnf, and their common region m-gdnf in the pons as well as the inferior (IC) and superior colliculi in Krushinsky–Molodkina (KM) rats and in the strain “0” was analyzed by quantitative real-time polymerase chain reaction (PCR) in the control (unstimulated KM and “0” rats) and 1.5, 4.5, and 8 h after auditory stimulation. Such stimulation induced audiogenic seizures (AS) in KM rats. Audiogenic seizure was not observed in “0” rats, which was obtained by selection for the absence of AS in a population of F2 hybrids between KM and Wistar rats not predisposed to AS. A significant drop in the level of all transcripts was observed 1.5 h after auditory stimulation in both KM and “0” rats. In most cases, the average expression of α and β isoforms and m-region 4.5 h after stimulation was greater than those after 1.5 and 8 h. At the same time, the expression of pre-(β)pro-gdnf in th