Аннотация:
Aim: to determinate the most effective liver cells and multipotent mesenchymal stromal cells of bone marrow (MMSC BM) ratio into implantable cell engineering constructions (CECs) used for chronic liver failure (CLF) correcting. Materials and methods. For creating liver CECs it was used a biopolymer implant – a composition of a heterogeneous collagen-containing gel (BMCG) (Sphero®GEL trademark) containing viable liver cells and MMSC BM in the following ratios – 1: 1; 5: 1 and 10: 1 respectively. CECs with different ratios of liver cells and MMSC BM were implanted into liver of rats in which chronic liver failure (CLF), was modeled by using CCl4. The effectiveness of the regulatory effects of CECs (with different cell ratios) on regenerative processes in livers were assessed by using biochemical, morphological and morphometric methods at different periods after their implantation. Results. Corrective effect of CECs with different cell composition on biochemical and morphological paramete
Ключевые слова:
Bioartificial organs; Biodegradable matrices; Cell transplantation; Cell-engineering construction; Liver; Liver failure; Regeneration
biopolymer; carbon tetrachloride; collagen; Article; bone marrow; cell engineering; cell viability; chronic liver failure; homeostasis; liver cell; liver donor; liver tissue; liver transplantation; mesenchymal stroma cell; multipotent stem cell
Шагидулин М. Ю. Мурат Юнусович 1958-
Онищенко Н. А. Нина Андреевна 1937-
Крашенинников М. Е. Михаил Евгеньевич 1959-
Николскайа А. О.
Волкова Е. А.
Ильинский И. М. Игорь Михайлович 1944-
Могеико Н. П.
Севастианов В. И.
Готье С. В. Сергей Владимирович 1947-
Shagidulin M. Yu. Murat Yunusovich 1958-
Onishhenko N. A. Nina Andreevna 1937-
Krasheninnikov M. E. Mikhail Evgenyevich 1959-
Nikolskaya A. O.
Volkova E. A.
Il`inskij I. M. Igor` Mikhaylovich 1944-
Mogeiko N. P.
Sevastianov V. I.
Gotye S. V. Sergej Vladimirovich 1947-
The influence of the ratio of liver cells and bone marrow in the implantable cell-engineering structures of the liver on the recovery efficiency of functional and morphological parameters in chronic liver failure [ВЛИЯНИЕ СООТНОШЕНИЯ КЛЕТОК ПЕЧЕНИ И ММСК КОСТНОГО МОЗГА В ИМПЛАНТИРУЕМЫХ КЛЕТОЧНО-ИНЖЕНЕРНЫХ КОНСТРУКЦИЯХ ПЕЧЕНИ НА ЭФФЕКТИВНОСТЬ ВОССТАНОВЛЕНИЯ ФУНКЦИОНАЛЬНЫХ И МОРФОЛОГИЧЕСКИХ ПОКАЗАТЕЛЕЙ ПРИ ХРОНИЧЕСКОЙ ПЕЧЕНОЧНОЙ НЕДОСТАТОЧНОСТИ]
The influence of the ratio of liver cells and bone marrow in the implantable cell-engineering structures of the liver on the recovery efficiency of...
Текст визуальный непосредственный
Вестник трансплантологии и искусственных органов
Федеральный научный центр трансплантологии и искусственных органов им. академика В.И. Шумакова
Т. 21, Вып. 1 С. 122-134
2019
Статья
Bioartificial organs Biodegradable matrices Cell transplantation Cell-engineering construction Liver Liver failure Regeneration
biopolymer carbon tetrachloride collagen Article bone marrow cell engineering cell viability chronic liver failure homeostasis liver cell liver donor liver tissue liver transplantation mesenchymal stroma cell multipotent stem cell
Aim: to determinate the most effective liver cells and multipotent mesenchymal stromal cells of bone marrow (MMSC BM) ratio into implantable cell engineering constructions (CECs) used for chronic liver failure (CLF) correcting. Materials and methods. For creating liver CECs it was used a biopolymer implant – a composition of a heterogeneous collagen-containing gel (BMCG) (Sphero®GEL trademark) containing viable liver cells and MMSC BM in the following ratios – 1: 1; 5: 1 and 10: 1 respectively. CECs with different ratios of liver cells and MMSC BM were implanted into liver of rats in which chronic liver failure (CLF), was modeled by using CCl4. The effectiveness of the regulatory effects of CECs (with different cell ratios) on regenerative processes in livers were assessed by using biochemical, morphological and morphometric methods at different periods after their implantation. Results. Corrective effect of CECs with different cell composition on biochemical and morphological paramete