Chemical and toxicological diagnosis of acute poisonings with phenazepam
Belova M. V., Klyuyev E. A., Mel`nikov E. S., Yeliseyeva D. M.
Журнал им. Н.В. Склифосовского Неотложная медицинская помощь
Vol.7, Issue4, P. 319-324
Опубликовано: 2018
Тип ресурса: Статья
DOI:10.23934/2223-9022-2018-7-4-319-324
Аннотация:
BACKGROUND The relative availability of Phenazepam makes it a frequent cause of overdose, suicide and non-medical use. At the same time, it remains insufficiently studied in chemical and toxicological terms. THE AIM OF STUDY to create an accessible, rapid method for detecting Phenazepam in biological matrices of patients with acute poisoning. MATERIALS AND METHODS We used thin-layer chromatography (TLC), gas chromatography with a mass selective detector (GC-MS), high performance liquid chromatography with a tandem mass-selective detector (LC-MS/MS) and immunochromatographic analysis (ICA). The preparation of samples of intact urine with the addition of standard solutions of Phenazepam and real urine samples of patients with acute poisoning with Phenazepam was carried out using liquid-liquid extraction or precipitation of related components of the sample with acetonitrile. Hydrolysis and derivatization were also added in GC-MS analysis. RESULTS The analysis of statistics of the Departme
Ключевые слова:
Acute poisoning; GC-MS; HPLC-MS/MS; Immune analysis; Phenazepam; Thin-layer chromatography
Язык текста: Английский
ISSN: 2541-8017
Belova M. V. Mariya Vladimirovna 1959-
Klyuyev E. A.
Mel`nikov E. S. Evgenij Sergeevich 1990-
Yeliseyeva D. M.
Белова М. В. Мария Владимировна 1959-
Клюьев Е. А.
Мельников Е. С. Евгений Сергеевич 1990-
Ьелисеьева Д. М.
Chemical and toxicological diagnosis of acute poisonings with phenazepam
Текст визуальный непосредственный
Журнал им. Н.В. Склифосовского Неотложная медицинская помощь
Научно-практическое общество врачей неотложной медицины
Vol.7, Issue4 P. 319-324
2018
Статья
Acute poisoning GC-MS HPLC-MS/MS Immune analysis Phenazepam Thin-layer chromatography
BACKGROUND The relative availability of Phenazepam makes it a frequent cause of overdose, suicide and non-medical use. At the same time, it remains insufficiently studied in chemical and toxicological terms. THE AIM OF STUDY to create an accessible, rapid method for detecting Phenazepam in biological matrices of patients with acute poisoning. MATERIALS AND METHODS We used thin-layer chromatography (TLC), gas chromatography with a mass selective detector (GC-MS), high performance liquid chromatography with a tandem mass-selective detector (LC-MS/MS) and immunochromatographic analysis (ICA). The preparation of samples of intact urine with the addition of standard solutions of Phenazepam and real urine samples of patients with acute poisoning with Phenazepam was carried out using liquid-liquid extraction or precipitation of related components of the sample with acetonitrile. Hydrolysis and derivatization were also added in GC-MS analysis. RESULTS The analysis of statistics of the Departme