Identification of Bordetella holmesii among the patients hospitalized with suspicion of pertussis and pertussis-like illnesses [ВЫЯВЛЕНИЕ...
Гадуа Н. Т., Борисова А. Б., Пименова А. С., Борисова О. Y., Петрова М. С., Шамшева О. В., Афанас'ев С. С., Кафарскайа Л. И., Власов Е. В., Афанасьев М. С., Алешкин А. В., Бунин С. В., Алешкин В. А.
Журнал инфектологии
Т. 11, Вып. 2, С. 45-52
Опубликовано: 2019
Тип ресурса: Статья
DOI:10.22625/2072-6732-2019-11-2-45-52
Аннотация:
Purpose. To reveal and estimate prevalence of B. holmesii among the patients hospitalized with suspicion pertussis and pertussis-like illnesses. Materials and methods. 424 clinical samples received from patients with of pertussis and pertussis-like illnesses in GBUZ IKB № 1 DZM in 2017-2018 are investigated. Identification of fragments of a genome of Bordetella was carried out in PCR-RT with “Amplisens® Bordetella multi-FL”. For identification of fragments of a genome of B. holmesii used PCR-RT with primers of IS481, IS1001 and hIS1001. Results. The research included 424 patients, from them 56,1[%] of children aged till 1 year, 41,3[%] of children - are more senior than 1 year and 2,6[%] of adults. When using test system 60,4[%] of the samples containing DNA of B. pertussis are revealed; 1,9[%] of samples - DNA of B. parapertussis; in 34,9[%] of samples it is received negative and in 2,8[%] - doubtful results. The research of 424 samples in PCR-RT by means of IS481, IS1001 and hIS1001 primers showed
Ключевые слова:
Age; B. holmesii; B. pertussis; Patients; PCR-RT; Whooping cough
bacterial DNA; Article; bacterial genome; bacterium identification; Bordetella holmesii; child; controlled study; human; major clinical study; nonhuman; pertussis; prevalence; real time polymerase chain reaction
Язык текста: Русский
ISSN: 2499-9865
Гадуа Н. Т.
Борисова А. Б.
Пименова А. С.
Борисова О. Y. О.Ю.
Петрова М. С.
Шамшева О. В.
Афанас'ев С. С.
Кафарскайа Л. И.
Власов Е. В.
Афанасьев М. С. Максим Станиславович 1978-
Алешкин А. В.
Бунин С. В.
Алешкин В. А.
Gadua N. T.
Borisova A. B.
Pimenova A. S.
Borisova O. Y. O.Yu.
Petrova M. S.
Shamsheva O. V.
Afanas'ev S. S.
Kafarskaya L. I.
Vlasov E. V.
Afanasyev M. S. Maksim Stanislavovich 1978-
Aleshkin A. V.
Bunin S. V.
Aleshkin V. A.
Identification of Bordetella holmesii among the patients hospitalized with suspicion of pertussis and pertussis-like illnesses [ВЫЯВЛЕНИЕ Bordetella holmesii СРЕДИ БОЛЬНЫХ, ГОСПИТАЛИЗИРОВАННЫХ В СТАЦИОНАР С ПОДОЗРЕНИЕМ НА КОКЛЮШ ИЛИ КОКЛЮШЕПОДОБНЫЕ ЗАБОЛЕВАНИЯ]
Identification of Bordetella holmesii among the patients hospitalized with suspicion of pertussis and pertussis-like illnesses [ВЫЯВЛЕНИЕ...
Текст визуальный непосредственный
Журнал инфектологии
Ассоциация врачей-инфекционистов Санкт-Петербурга и Ленинградской области
Т. 11, Вып. 2 С. 45-52
2019
Статья
Age B. holmesii B. pertussis Patients PCR-RT Whooping cough
bacterial DNA Article bacterial genome bacterium identification Bordetella holmesii child controlled study human major clinical study nonhuman pertussis prevalence real time polymerase chain reaction
Purpose. To reveal and estimate prevalence of B. holmesii among the patients hospitalized with suspicion pertussis and pertussis-like illnesses. Materials and methods. 424 clinical samples received from patients with of pertussis and pertussis-like illnesses in GBUZ IKB № 1 DZM in 2017-2018 are investigated. Identification of fragments of a genome of Bordetella was carried out in PCR-RT with “Amplisens® Bordetella multi-FL”. For identification of fragments of a genome of B. holmesii used PCR-RT with primers of IS481, IS1001 and hIS1001. Results. The research included 424 patients, from them 56,1[%] of children aged till 1 year, 41,3[%] of children - are more senior than 1 year and 2,6[%] of adults. When using test system 60,4[%] of the samples containing DNA of B. pertussis are revealed; 1,9[%] of samples - DNA of B. parapertussis; in 34,9[%] of samples it is received negative and in 2,8[%] - doubtful results. The research of 424 samples in PCR-RT by means of IS481, IS1001 and hIS1001 primers showed