Hydrogel microchip as a tool for studying exosomes in human serum
Бутвиловскайа В. И., Тихонов А. А., Савватеева Е. Н., Рагимов А. А. о., Салимов Э. Л., Волошин С. А., Сидоров Д. В., Черниченко М. А., Поляков А. П., Филушин М. М., Цyбулскайа М. В., Рубина А. Y.
Молекулярная биология
Т. 51, Вып. 5, С. 817-823
Опубликовано: 2017
Тип ресурса: Статья
DOI:10.7868/S0026898417050081
Аннотация:
Exosomes are cell-derived vesicles that are secreted by both normal and cancer cells. Over the last decade, a few studies have revealed that exosomes cross talk and/or influence major tumor-related pathways such as angiogenesis and metastasis involving many cell types within the tumor microenvironment. The protein composition of the membrane of an exosome reflects that of the membrane of the cell of origin. Because of this, tumor-derived exosomes differ from exosomes that are derived from normal cells. The detection of tumor exosomes and analysis of their molecular composition hold promise for diagnosis and prognosis of cancer. Here, we present hydrogel microarrays (biochips), which contain a panel of immobilized antibodies that recognize tetraspanins (CD9, CD63, CD81) and prognostic markers for colorectal cancer (A33, CD147). These biochips make it possible to analyze the surface proteins of either isolated exosomes or exosomes that are present in the serum samples without isolation.
Ключевые слова:
CD antigen; exosome detection; exosomes; Hydrogel; microvesicles; tetraspanins
leukocyte antigen; tumor marker; tumor protein; adult; blood; chemistry; colorectal tumor; exosome; female; human; hydrogel; lab on a chip; male; metabolism; microchip analysis; middle aged; procedures; Adult; Antigens, CD; Biomarkers, Tumor; Colorectal Neoplasms; Exosomes; Female; Humans; Hydrogels; Lab-On-A-Chip Devices; Male; Microchip Analytical Procedures; Middle Aged; Neoplasm Proteins
Язык текста: Русский
ISSN: 0026-8984
Бутвиловскайа В. И.
Тихонов А. А.
Савватеева Е. Н.
Рагимов А. А. о. Алигейдар Агаалекпер оглы 1950-
Салимов Э. Л. Эмин Львович 1972-
Волошин С. А.
Сидоров Д. В.
Черниченко М. А.
Поляков А. П.
Филушин М. М.
Цyбулскайа М. В.
Рубина А. Y.
Butvilovskaya V. I.
Tikhonov A. A.
Savvateeva E. N.
Ragimov A. A. o. Aligejdar Agaalekper ogly' 1950-
Salimov E`. L. E`min L`vovich 1972-
Voloshin S. A.
Sidorov D. V.
Chernichenko M. A.
Polyakov A. P.
Filushin M. M.
Tsybulskaya M. V.
Rubina A. Y.
Hydrogel microchip as a tool for studying exosomes in human serum
Текст визуальный непосредственный
Молекулярная биология
Российская академия наук
Т. 51, Вып. 5 С. 817-823
2017
Статья
CD antigen exosome detection exosomes Hydrogel microvesicles tetraspanins
leukocyte antigen tumor marker tumor protein adult blood chemistry colorectal tumor exosome female human hydrogel lab on a chip male metabolism microchip analysis middle aged procedures Adult Antigens, CD Biomarkers, Tumor Colorectal Neoplasms Exosomes Female Humans Hydrogels Lab-On-A-Chip Devices Male Microchip Analytical Procedures Middle Aged Neoplasm Proteins
Exosomes are cell-derived vesicles that are secreted by both normal and cancer cells. Over the last decade, a few studies have revealed that exosomes cross talk and/or influence major tumor-related pathways such as angiogenesis and metastasis involving many cell types within the tumor microenvironment. The protein composition of the membrane of an exosome reflects that of the membrane of the cell of origin. Because of this, tumor-derived exosomes differ from exosomes that are derived from normal cells. The detection of tumor exosomes and analysis of their molecular composition hold promise for diagnosis and prognosis of cancer. Here, we present hydrogel microarrays (biochips), which contain a panel of immobilized antibodies that recognize tetraspanins (CD9, CD63, CD81) and prognostic markers for colorectal cancer (A33, CD147). These biochips make it possible to analyze the surface proteins of either isolated exosomes or exosomes that are present in the serum samples without isolation.