Population-Based Analysis of Cluster Headache-Associated Genetic Polymorphisms

Katsarou M. -., Papasavva M., Latsi R., Toliza I., Gkaros A. -., Papakonstantinou S., Gatzonis S., Mitsikostas D. -., Kovatsi L., Isotov B. N., Tsatsakis A., Drakoulis N.

Journal of Molecular Neuroscience

Vol.65, Issue3, P. 367-376

Опубликовано: 2018

Тип ресурса: Статья

DOI:10.1007/s12031-018-1103-5

Аннотация:

Cluster headache is a disorder with increased hereditary risk. Associations between cluster headache and polymorphism rs2653349 of the HCRTR2 gene have been demonstrated. The less common allele (A) seems to reduce disease susceptibility. The polymorphism rs5443 of the GNB3 gene positively influences triptan treatment response. Carriers of the mutated T allele are more likely to respond positively compared to C:C homozygotes, when treated with triptans. DNA was extracted from buccal swabs obtained from 636 non-related Southeastern European Caucasian individuals and was analyzed by real-time PCR. Gene distribution for the rs2653349 was G:G = 79.1[%], G:A = 19.2[%], and A:A = 1.7[%]. The frequency of the wild-type G allele was 88.7[%]. The frequencies for rs5443 were C:C = 44.0[%], C:T = 42.6[%], and T:T = 13.4[%]. The frequency of the wild-type C allele was 65.3[%]. The frequency distribution of rs2653349 in the Southeastern European Caucasian population differs significantly when compared with other Eu

Ключевые слова:

Cluster headache therapy; GNB3; HCRTR2; Pharmacogenomics; Southeastern European Caucasians

DNA; guanine nucleotide binding protein (G protein), beta polypeptide 3, human; HCRTR2 protein, human; heterotrimeric guanine nucleotide binding protein; orexin receptor; adult; African; aged; allele; Article; cluster headache; East Asian; European; female; gender; gene; genetic association; genetic polymorphism; genotype; gnb3 gene; human; major clinical study; male; population research; real time polymerase chain reaction; South Asian; wild type; ancestry group; cluster headache; ethnology; genetics; single nucleotide polymorphism; Adult; Cluster Headache; Continental Population Groups; Female; Heterotrimeric GTP-Binding Proteins; Humans; Male; Orexin Receptors; Polymorphism, Single Nucleotide

Язык текста: Английский

ISSN: 1559-1166

Katsarou M. -. M.-S.

Papasavva M.

Latsi R.

Toliza I.

Gkaros A. -. A.-P.

Papakonstantinou S.

Gatzonis S.

Mitsikostas D. -. D.-D.

Kovatsi L.

Isotov B. N.

Tsatsakis A. Aristidis 1957-

Drakoulis N.

Кацароу М. -. М.-С.

Папасавва М.

Лаци Р.

Толиза И.

Гкарос А. -. А.-П.

Папаконстантиноу С.

Гатзонис С.

Мицикостас Д. -. Д.-Д.

Коваци Л.

Исотов Б. Н.

Цацакис А. Аристидис 1957-

Дракоулис Н.

Population-Based Analysis of Cluster Headache-Associated Genetic Polymorphisms

Текст визуальный непосредственный

Journal of Molecular Neuroscience

Humana Press, Inc.

Vol.65, Issue3 P. 367-376

2018

Статья

Cluster headache therapy GNB3 HCRTR2 Pharmacogenomics Southeastern European Caucasians

DNA guanine nucleotide binding protein (G protein), beta polypeptide 3, human HCRTR2 protein, human heterotrimeric guanine nucleotide binding protein orexin receptor adult African aged allele Article cluster headache East Asian European female gender gene genetic association genetic polymorphism genotype gnb3 gene human major clinical study male population research real time polymerase chain reaction South Asian wild type ancestry group cluster headache ethnology genetics single nucleotide polymorphism Adult Cluster Headache Continental Population Groups Female Heterotrimeric GTP-Binding Proteins Humans Male Orexin Receptors Polymorphism, Single Nucleotide

Cluster headache is a disorder with increased hereditary risk. Associations between cluster headache and polymorphism rs2653349 of the HCRTR2 gene have been demonstrated. The less common allele (A) seems to reduce disease susceptibility. The polymorphism rs5443 of the GNB3 gene positively influences triptan treatment response. Carriers of the mutated T allele are more likely to respond positively compared to C:C homozygotes, when treated with triptans. DNA was extracted from buccal swabs obtained from 636 non-related Southeastern European Caucasian individuals and was analyzed by real-time PCR. Gene distribution for the rs2653349 was G:G = 79.1[%], G:A = 19.2[%], and A:A = 1.7[%]. The frequency of the wild-type G allele was 88.7[%]. The frequencies for rs5443 were C:C = 44.0[%], C:T = 42.6[%], and T:T = 13.4[%]. The frequency of the wild-type C allele was 65.3[%]. The frequency distribution of rs2653349 in the Southeastern European Caucasian population differs significantly when compared with other Eu