A rational approach to obtaining high-specific polyclonal antibodies against recombinant alpha-synuclein
Баринова К. В., Мелникова А. К., Счмалхаусен Е. В., Муронетз В. И.
Биомедицинская химия
Т. 64, Вып. 3, С. 276-282
Опубликовано: 2018
Тип ресурса: Статья
DOI:10.18097/PBMC20186403276
Аннотация:
The approach for the quick and efficient production of polyclonal antibodies to the target antigen alpha-synuclein has been proposed. Two methods have been employed to purify specific rabbit polyclonal antibodies against recombinant human alpha-synuclein, produced by subcutaneous immunization with complete Freund's adjuvant. It was shown that purification on CNBr-activated Sepharose with immobilized alpha-synuclein resulted in antibody preparation with rabbit serum histidine-rich glycoprotein as a contaminant. Two-stage antibody purification procedure first on Sepharose with immobilized protein G, and then on alpha-synuclein immobilized column helps to avoid contamination and to obtain homogenous antibody preparation. Antibodies recognize different conformations of alpha-synuclein and can be used in a variety of immunochemical approaches, including immunocytochemistry. © 2018 Russian Academy of Medical Sciences. All rights reserved.
Ключевые слова:
Adjuvant; Alpha-synuclein; Immunization; Polyclonal antibodies
alpha synuclein; antibody; recombinant protein; SNCA protein, human; animal; antibody specificity; human; immunization; immunology; Leporidae; alpha-Synuclein; Animals; Antibodies; Antibody Specificity; Humans; Immunization; Rabbits; Recombinant Proteins
Язык текста: Русский
ISSN: 2310-6905
Баринова К. В. Ксения Вячеславовна 1991-
Мелникова А. К.
Счмалхаусен Е. В.
Муронетз В. И.
Barinova K. V. Kseniya Vyacheslavovna 1991-
Melnikova A. K.
Schmalhausen E. V.
Muronetz V. I.
A rational approach to obtaining high-specific polyclonal antibodies against recombinant alpha-synuclein
Текст визуальный непосредственный
Биомедицинская химия
Научно-исследовательский институт биомедицинской химии им. В.Н. Ореховича
Т. 64, Вып. 3 С. 276-282
2018
Статья
Adjuvant Alpha-synuclein Immunization Polyclonal antibodies
alpha synuclein antibody recombinant protein SNCA protein, human animal antibody specificity human immunization immunology Leporidae alpha-Synuclein Animals Antibodies Antibody Specificity Humans Immunization Rabbits Recombinant Proteins
The approach for the quick and efficient production of polyclonal antibodies to the target antigen alpha-synuclein has been proposed. Two methods have been employed to purify specific rabbit polyclonal antibodies against recombinant human alpha-synuclein, produced by subcutaneous immunization with complete Freund's adjuvant. It was shown that purification on CNBr-activated Sepharose with immobilized alpha-synuclein resulted in antibody preparation with rabbit serum histidine-rich glycoprotein as a contaminant. Two-stage antibody purification procedure first on Sepharose with immobilized protein G, and then on alpha-synuclein immobilized column helps to avoid contamination and to obtain homogenous antibody preparation. Antibodies recognize different conformations of alpha-synuclein and can be used in a variety of immunochemical approaches, including immunocytochemistry. © 2018 Russian Academy of Medical Sciences. All rights reserved.