Аннотация:
Apoptosis, as the major type of programmed cell death, plays an important role in the organism renewal and removal of defective and transformed cells, including cancer cells. One of the earliest apoptotic events is lipid peroxidation in the inner mitochondrial membrane catalyzed by a complex of cytochrome c (CytC) with the mitochondrial phospholipid cardiolipin (CL). It was shown that mixing CytC and CL solutions results in the formation of CytC/CL complexes (Cyt-CL nanospheres) with a diameter of 11–12 nm composed of the molten globule protein molecule and a CL monolayer. Using the methods of dynamic light scattering for the Cyt-CL chloroform solution and small-angle X-ray scattering for the Cyt-CL sediment, it was found that in both cases, Cyt-CL formed nanospheres with a diameter of 8 and 11 nm, which corresponded to the earlier determined lipid/protein ratios of 13–14 and 35–50, respectively. These results showed that the Cyt-CL nanospheres can form not only during crystallization
Ключевые слова:
apoptosis; cardiolipin; Cyt-CL complex; cytochrome c; dynamic light scattering; hydrophobic medium; molten globule; small-angle X-ray scattering
cardiolipin; cytochrome c; heme; methanol; methionine; nanosphere; animal; apoptosis; chemistry; crystallization; fluorescence resonance energy transfer; heart mitochondrion; horse; hydrogen bond; lipid bilayer; lipid peroxidation; metabolism; mitochondrial membrane; pH; photon correlation spectroscopy; physiology; protein conformation; protein unfolding; Animals; Apoptosis; Cardiolipins; Crystallization; Cytochromes c; Dynamic Light Scattering; Fluorescence Resonance Energy Transfer; Heme; Horses; Hydrogen Bonding; Hydrogen-Ion Concentration; Lipid Bilayers; Lipid Peroxidation; Methanol; Methionine; Mitochondria, Heart; Mitochondrial Membranes; Nanospheres; Protein Conformation; Protein Unfolding
Vladimirov G. K. Georgij Konstantinovich 1992-
Remenshchikov V. E.
Nesterova A. M. Anastasiya Mikhaylovna 1988-
Volkov V. V.
Vladimirov Yu. A. Yurij Andreevich 1932-
Владимиров Г. К. Георгий Константинович 1992-
Ременшчиков В. Е.
Нестерова А. М. Анастасия Михайловна 1988-
Волков В. В.
Владимиров Ю. А. Юрий Андреевич 1932-
Comparison of the Size and Properties of the Cytochrome c/Cardiolipin Nanospheres in a Sediment and Non-polar Medium
Текст визуальный непосредственный
Biochemistry (Moscow)
Pleiades Publishing, Ltd.
Vol.84, Issue8 P. 923-930
2019
Статья
apoptosis cardiolipin Cyt-CL complex cytochrome c dynamic light scattering hydrophobic medium molten globule small-angle X-ray scattering
cardiolipin cytochrome c heme methanol methionine nanosphere animal apoptosis chemistry crystallization fluorescence resonance energy transfer heart mitochondrion horse hydrogen bond lipid bilayer lipid peroxidation metabolism mitochondrial membrane pH photon correlation spectroscopy physiology protein conformation protein unfolding Animals Apoptosis Cardiolipins Crystallization Cytochromes c Dynamic Light Scattering Fluorescence Resonance Energy Transfer Heme Horses Hydrogen Bonding Hydrogen-Ion Concentration Lipid Bilayers Lipid Peroxidation Methanol Methionine Mitochondria, Heart Mitochondrial Membranes Nanospheres Protein Conformation Protein Unfolding
Apoptosis, as the major type of programmed cell death, plays an important role in the organism renewal and removal of defective and transformed cells, including cancer cells. One of the earliest apoptotic events is lipid peroxidation in the inner mitochondrial membrane catalyzed by a complex of cytochrome c (CytC) with the mitochondrial phospholipid cardiolipin (CL). It was shown that mixing CytC and CL solutions results in the formation of CytC/CL complexes (Cyt-CL nanospheres) with a diameter of 11–12 nm composed of the molten globule protein molecule and a CL monolayer. Using the methods of dynamic light scattering for the Cyt-CL chloroform solution and small-angle X-ray scattering for the Cyt-CL sediment, it was found that in both cases, Cyt-CL formed nanospheres with a diameter of 8 and 11 nm, which corresponded to the earlier determined lipid/protein ratios of 13–14 and 35–50, respectively. These results showed that the Cyt-CL nanospheres can form not only during crystallization