Optimized bioinformatic strategy for the analysis of clinical proteomic data of the endometrium in chronic endometritis
Гаинуллин М. Р., Язyкова А. Б., Мотовилова Т. М., Апумаита Х. М. К., Ходосова Т. Г., Гагаева Y. А., Коломина Е. С., Ковалева М. М., Милицкайа А. А., Шчерина А. Н., Боyко Е. Л., Згода В. Г., Гречканев Г. О.
Современные технологии в медицине
Т. 11, Вып. 2, С. 50-54
Опубликовано: 2019
Тип ресурса: Статья
DOI:10.17691/stm2019.11.2.07
Аннотация:
The aim of the study is to analyze the entire set of proteins (proteome) expressed in the endometrial tissue and to identify protein markers specific for carcinogenesis. Materials and Methods. Tissue samples were obtained using endometrial pipelle biopsy in women with chronic endometritis. After homogenization the samples were subjected to protein electrophoresis in polyacrylamide gel in the presence of sodium dodecyl sulfate according to the Lamley method. The proteins separated according to their molecular weights were digested by modified trypsin using the standard method. Obtained tryptic peptides were analyzed and identified by high-performance liquid chromatography coupled with tandem mass spectrometry. The Human Protein Atlas and Tissue-Specific Gene Expression and Regulation databases were used to analyze the tissue-specific protein expression. Functional protein annotation and gene set enrichment analysis were performed using the Database for Annotation, Visualization and Inte
Ключевые слова:
Chronic endometritis; Functional clustering; Tandem mass spectrometry; Tissue-specific expression
annexin; ceruloplasmin; cofilin; complement component C3; complement component C4a; enolase; filamin A; glucose 6 phosphate isomerase; glutathione transferase; glyceraldehyde 3 phosphate dehydrogenase; glycogen phosphorylase; hemoglobin beta chain; heterogeneous nuclear ribonucleoprotein D; high mobility group B1 protein; inorganic pyrophosphatase; lactate dehydrogenase isoenzyme; lipocortin 5; myosin heavy chain; peroxiredoxin 6; procollagen proline 2 oxoglutarate 4 dioxygenase; profilin; protein disulfide isomerase; protein glutamine gamma glutamyltransferase; proteome; proton transporting adenosine triphosphate synthase; SUMO 1 protein; transaldolase; transketolase; tripeptidyl peptidase I; unindexed drug; Article; bioinformatics; carcinogenesis; controlled study; cystadenoma; endometritis; endometrium biopsy; female; gene expression; histology; human; human tissue; immunoglobulin constant region; liquid chromatography-mass spectrometry; molecular weight; ovary carcinoma; pilot stud
Язык текста: Русский
ISSN: 2076-4243
Гаинуллин М. Р.
Язyкова А. Б.
Мотовилова Т. М.
Апумаита Х. М. К.
Ходосова Т. Г.
Гагаева Y. А.
Коломина Е. С.
Ковалева М. М.
Милицкайа А. А.
Шчерина А. Н.
Боyко Е. Л.
Згода В. Г.
Гречканев Г. О.
Gainullin M. R.
Yazykova A. B.
Motovilova T. M.
Apumaita H. M. K.
Khodosova T. G.
Gagaeva Y. A.
Kolomina E. S.
Kovaleva M. M.
Militskaya A. A.
Shcherina A. N.
Boyko E. L.
Zgoda V. G.
Grechkanev G. O.
Optimized bioinformatic strategy for the analysis of clinical proteomic data of the endometrium in chronic endometritis
Текст визуальный непосредственный
Современные технологии в медицине
Нижегородская государственная медицинская академия
Т. 11, Вып. 2 С. 50-54
2019
Статья
Chronic endometritis Functional clustering Tandem mass spectrometry Tissue-specific expression
annexin ceruloplasmin cofilin complement component C3 complement component C4a enolase filamin A glucose 6 phosphate isomerase glutathione transferase glyceraldehyde 3 phosphate dehydrogenase glycogen phosphorylase hemoglobin beta chain heterogeneous nuclear ribonucleoprotein D high mobility group B1 protein inorganic pyrophosphatase lactate dehydrogenase isoenzyme lipocortin 5 myosin heavy chain peroxiredoxin 6 procollagen proline 2 oxoglutarate 4 dioxygenase profilin protein disulfide isomerase protein glutamine gamma glutamyltransferase proteome proton transporting adenosine triphosphate synthase SUMO 1 protein transaldolase transketolase tripeptidyl peptidase I unindexed drug Article bioinformatics carcinogenesis controlled study cystadenoma endometritis endometrium biopsy female gene expression histology human human tissue immunoglobulin constant region liquid chromatography-mass spectrometry molecular weight ovary carcinoma pilot stud
The aim of the study is to analyze the entire set of proteins (proteome) expressed in the endometrial tissue and to identify protein markers specific for carcinogenesis. Materials and Methods. Tissue samples were obtained using endometrial pipelle biopsy in women with chronic endometritis. After homogenization the samples were subjected to protein electrophoresis in polyacrylamide gel in the presence of sodium dodecyl sulfate according to the Lamley method. The proteins separated according to their molecular weights were digested by modified trypsin using the standard method. Obtained tryptic peptides were analyzed and identified by high-performance liquid chromatography coupled with tandem mass spectrometry. The Human Protein Atlas and Tissue-Specific Gene Expression and Regulation databases were used to analyze the tissue-specific protein expression. Functional protein annotation and gene set enrichment analysis were performed using the Database for Annotation, Visualization and Inte