Neuroprotective effects of Scrophularia buergeriana extract against glutamate-induced toxicity in SH-SY5Y cells
Lee H. J., Spandidos D. A., Tsatsakis A., Margina D., Izotov B. N., Yang S. H.
International journal of molecular medicine
Vol.43, Issue5, P. 2144-2152
Опубликовано: 2019
Тип ресурса: Статья
DOI:10.3892/ijmm.2019.4139
Аннотация:
The aim of this study was to investigate the antioxidant and anti-apoptotic activities, as well as the underlying mechanisms of action, of Scrophularia buergeriana (S. buer‑ geriana) extract (SBE) in glutamate-induced SH-SY5Y cell death. The roots of S. buergeriana were extracted with 70[%] ethanol, and standardized SBE was used in this study. To induce cytotoxicity, the SH-SY5Y cells were exposed to glutamate for 3 h, or pre-treated with SBE for 1 h, and subsequently incubated with glutamate for 3 h. The neuroprotective effects were assessed by measuring cell viability and the total glutathione contents using commercial kits. The antioxidant and anti-apoptotic mechanisms of action of SBE were evaluated by western blot analysis. The results confirmed that glutamate-induced toxicity was caused by reactive oxygen species (ROS) production, leading to oxidative stress and DNA damage, thus leading to cell death. However, treatment of the SH‑SY5Y cells with SBE significantly increased the viab
Ключевые слова:
Anti-apoptotic; Glutamate; Oxidative stress; Scropularia buergeriana; SH-SY5Y cells
alcohol; caspase 3; copper zinc superoxide dismutase; glutamic acid; glutathione; glutathione peroxidase 1; manganese superoxide dismutase; mitogen activated protein kinase; neuroprotective agent; nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase; plant extract; protein Bax; protein bcl 2; reactive oxygen metabolite; Scrophularia buergeriana extract; unclassified drug; acetylcholinesterase; antioxidant; glutamic acid; glutathione; mitogen activated protein kinase p38; neuroprotective agent; plant extract; reactive oxygen metabolite; antiapoptotic activity; antioxidant activity; Article; cell death; cell level; cell viability; controlled study; cytotoxicity; DNA damage; human; human cell; incubation time; long term exposure; neuroprotection; oxidative stress; plant root; priority journal; protein expression; Scrophularia; Scrophularia buergeriana; SH-SY5Y cell line; upregulation; Western blotting; apoptosis; chemistry; DNA fragmentation; drug effect; metabolism
Язык текста: Английский
ISSN: 1791-244X
Lee H. J.
Spandidos D. A.
Tsatsakis A. Aristidis 1957-
Margina D.
Izotov B. N. Boris Nikolaevich 1938-
Yang S. H.
Лее Х. Й.
Спандидос Д. А.
Цацакис А. Аристидис 1957-
Маргина Д.
Изотов Б. Н. Борис Николаевич 1938-
Янг С. Х.
Neuroprotective effects of Scrophularia buergeriana extract against glutamate-induced toxicity in SH-SY5Y cells
Текст визуальный непосредственный
International journal of molecular medicine
Spandidos Publications Ltd.
Vol.43, Issue5 P. 2144-2152
2019
Статья
Anti-apoptotic Glutamate Oxidative stress Scropularia buergeriana SH-SY5Y cells
alcohol caspase 3 copper zinc superoxide dismutase glutamic acid glutathione glutathione peroxidase 1 manganese superoxide dismutase mitogen activated protein kinase neuroprotective agent nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase plant extract protein Bax protein bcl 2 reactive oxygen metabolite Scrophularia buergeriana extract unclassified drug acetylcholinesterase antioxidant glutamic acid glutathione mitogen activated protein kinase p38 neuroprotective agent plant extract reactive oxygen metabolite antiapoptotic activity antioxidant activity Article cell death cell level cell viability controlled study cytotoxicity DNA damage human human cell incubation time long term exposure neuroprotection oxidative stress plant root priority journal protein expression Scrophularia Scrophularia buergeriana SH-SY5Y cell line upregulation Western blotting apoptosis chemistry DNA fragmentation drug effect metabolism
The aim of this study was to investigate the antioxidant and anti-apoptotic activities, as well as the underlying mechanisms of action, of Scrophularia buergeriana (S. buer‑ geriana) extract (SBE) in glutamate-induced SH-SY5Y cell death. The roots of S. buergeriana were extracted with 70[%] ethanol, and standardized SBE was used in this study. To induce cytotoxicity, the SH-SY5Y cells were exposed to glutamate for 3 h, or pre-treated with SBE for 1 h, and subsequently incubated with glutamate for 3 h. The neuroprotective effects were assessed by measuring cell viability and the total glutathione contents using commercial kits. The antioxidant and anti-apoptotic mechanisms of action of SBE were evaluated by western blot analysis. The results confirmed that glutamate-induced toxicity was caused by reactive oxygen species (ROS) production, leading to oxidative stress and DNA damage, thus leading to cell death. However, treatment of the SH‑SY5Y cells with SBE significantly increased the viab