Proteomic analysis of mouse brain subjected to spaceflight
Mao X. W., Sandberg L. B., Gridley D. S., Herrmann E. C., Zhang G., Raghavan R., Zubarev R. A., Zhang B., Stodieck L. S., Ferguson V. L., Bateman T. A., Pecaut M. J.
International Journal of Molecular Sciences
Vol.20, Issue1, Num.7
Опубликовано: 2019
Тип ресурса: Статья
Аннотация:
There is evidence that spaceflight poses acute and late risks to the central nervous system. To explore possible mechanisms, the proteomic changes following spaceflight in mouse brain were characterized. Space Shuttle Atlantis (STS-135) was launched from the Kennedy Space Center (KSC) on a 13-day mission. Within 3–5 h after landing, brain tissue was collected to evaluate protein expression profiles using quantitative proteomic analysis. Our results showed that there were 26 proteins that were significantly altered after spaceflight in the gray and/or white matter. While there was no overlap between the white and gray matter in terms of individual proteins, there was overlap in terms of function, synaptic plasticity, vesical activity, protein/organelle transport, and metabolism. Our data demonstrate that exposure to the spaceflight environment induces significant changes in protein expression related to neuronal structure and metabolic function. This might lead to a significant impact o
Ключевые слова:
Brain; Microgravity; Proteomics; Spaceflight
animal experiment; article; brain tissue; cell organelle; gene expression profiling; gray matter; microgravity; mouse; nerve cell plasticity; nonhuman; protein expression; protein function; proteomics; quantitative analysis; space flight; structure activity relation; white matter; animal; brain; female; glycolysis; gray matter; intracellular space; metabolism; metabolome; mitochondrion; oxidative stress; procedures; signal transduction; weightlessness; Animals; Brain; Female; Glycolysis; Gray Matter; Intracellular Space; Metabolome; Mice; Mitochondria; Oxidative Stress; Proteomics; Signal Transduction; Space Flight; Weightlessness; White Matter
Язык текста: Английский
ISSN: 1661-6596
Mao X. W.
Sandberg L. B.
Gridley D. S.
Herrmann E. C.
Zhang G.
Raghavan R.
Zubarev R. A. Roman Aleksandrovich 1963-
Zhang B.
Stodieck L. S.
Ferguson V. L.
Bateman T. A.
Pecaut M. J.
Мао Х. W.
Сандберг Л. Б.
Гридлеy Д. С.
Херрманн Е. C.
Жанг Г.
Рагхаван Р.
Зубарев Р. А. Роман Александрович 1963-
Жанг Б.
Стодиеcк Л. С.
Фергусон В. Л.
Батеман Т. А.
Пеcаут М. Й.
Proteomic analysis of mouse brain subjected to spaceflight
Текст визуальный непосредственный
International Journal of Molecular Sciences
Vol.20, Issue1 Num.7
2019
Статья
Brain Microgravity Proteomics Spaceflight
animal experiment article brain tissue cell organelle gene expression profiling gray matter microgravity mouse nerve cell plasticity nonhuman protein expression protein function proteomics quantitative analysis space flight structure activity relation white matter animal brain female glycolysis gray matter intracellular space metabolism metabolome mitochondrion oxidative stress procedures signal transduction weightlessness Animals Brain Female Glycolysis Gray Matter Intracellular Space Metabolome Mice Mitochondria Oxidative Stress Proteomics Signal Transduction Space Flight Weightlessness White Matter
There is evidence that spaceflight poses acute and late risks to the central nervous system. To explore possible mechanisms, the proteomic changes following spaceflight in mouse brain were characterized. Space Shuttle Atlantis (STS-135) was launched from the Kennedy Space Center (KSC) on a 13-day mission. Within 3–5 h after landing, brain tissue was collected to evaluate protein expression profiles using quantitative proteomic analysis. Our results showed that there were 26 proteins that were significantly altered after spaceflight in the gray and/or white matter. While there was no overlap between the white and gray matter in terms of individual proteins, there was overlap in terms of function, synaptic plasticity, vesical activity, protein/organelle transport, and metabolism. Our data demonstrate that exposure to the spaceflight environment induces significant changes in protein expression related to neuronal structure and metabolic function. This might lead to a significant impact o