Extracellular Matrix Determines Biomechanical Properties of Chondrospheres during Their Maturation In Vitro
Omelyanenko N. P., Karalkin P. A., Bulanova E. A., Koudan E. V., Parfenov V. A., Rodionov S. A., Knyazeva A. D., Kasyanov V. A., Babichenko I. I., Chkadua T. Z., Khesuani Y. D., Gryadunova A. A., Mironov V. A.
Cartilage
Vol.11, Issue4, P. 521-531
Опубликовано: 2020
Тип ресурса: Статья
DOI:10.1177/1947603518798890
Аннотация:
Objective: Chondrospheres represent a variant of tissue spheroids biofabricated from chondrocytes. They are already being used in clinical trials for cartilage repair; however, their biomechanical properties have not been systematically investigated yet. The aim of our study was to characterize chondrospheres in long-term in vitro culture conditions for morphometric changes, biomechanical integrity, and their fusion and spreading kinetics. Results: It has been demonstrated that the increase in chondrospheres secant modulus of elasticity is strongly associated with the synthesis and accumulation of extracellular matrix. Additionally, significant interplay has been found between biomechanical properties of tissue spheroids and their fusion kinetics in contrast to their spreading kinetics. Conclusions: Extracellular matrix is one of the main structural determinants of chondrospheres biomechanical properties during chondrogenic maturation in vitro. The estimation of tissue spheroids’ physi
Ключевые слова:
biomechanical properties; cartilage; chondrospheres; extracellular matrix; tissue spheroids
article; cartilage; controlled study; elasticity; extracellular matrix; human; human cell; human tissue; implantation; in vivo study; kinetics; maturation; synthesis; tumor spheroid
Язык текста: Английский
ISSN: 1947-6043
Omelyanenko N. P.
Karalkin P. A.
Bulanova E. A.
Koudan E. V.
Parfenov V. A.
Rodionov S. A.
Knyazeva A. D.
Kasyanov V. A.
Babichenko I. I.
Chkadua T. Z.
Khesuani Y. D.
Gryadunova A. A. Anna Aleksandrovna 1992-
Mironov V. A. Vladimir Aleksandrovich 1954-
Омеляненко Н. П.
Каралкин П. А.
Буланова Е. А.
Коудан Е. В.
Парфенов В. А.
Родионов С. А.
Князева А. Д.
Касянов В. А.
Бабиченко И. И.
Чкадуа Т. З.
Хесуани Y. Д.
Грядунова А. А. Анна Александровна 1992-
Миронов В. А. Владимир Александрович 1954-
Extracellular Matrix Determines Biomechanical Properties of Chondrospheres during Their Maturation In Vitro
Текст визуальный непосредственный
Cartilage
Vol.11, Issue4 P. 521-531
2020
Статья
biomechanical properties cartilage chondrospheres extracellular matrix tissue spheroids
article cartilage controlled study elasticity extracellular matrix human human cell human tissue implantation in vivo study kinetics maturation synthesis tumor spheroid
Objective: Chondrospheres represent a variant of tissue spheroids biofabricated from chondrocytes. They are already being used in clinical trials for cartilage repair; however, their biomechanical properties have not been systematically investigated yet. The aim of our study was to characterize chondrospheres in long-term in vitro culture conditions for morphometric changes, biomechanical integrity, and their fusion and spreading kinetics. Results: It has been demonstrated that the increase in chondrospheres secant modulus of elasticity is strongly associated with the synthesis and accumulation of extracellular matrix. Additionally, significant interplay has been found between biomechanical properties of tissue spheroids and their fusion kinetics in contrast to their spreading kinetics. Conclusions: Extracellular matrix is one of the main structural determinants of chondrospheres biomechanical properties during chondrogenic maturation in vitro. The estimation of tissue spheroids’ physi