Development of a low cost gas diffusion device for ammonia detection in the vitreous humor and its preliminary application for estimation of the...
Musile D., Gottardo R., Palacio C., Shestakova K. M., Raniero D., De P. E. F., Tal`yaro F.
Forensic Science International
Vol.295, P. 150-156
Опубликовано: 2019
Тип ресурса: Статья
DOI:10.1016/j.forsciint.2018.12.012
Аннотация:
A simple and low cost analytical device is described for the determination of ammonium in the vitreous humor suitable for inferring the post mortem interval in forensic cases. The device is based on ammonia formation from ammonium ion by means of NaOH addition to the vitreous humor sample and its detection with a pH chemical indicator in the gas phase above the vitreous humor sample. From the gas phase, ammonia diffuses through a polymeric membrane and it is trapped and detected with a droplet of pH indicator thymol blue. The color change of the droplet is measured using a smartphone camera. Under optimal conditions, the device showed a limit of detection of 0.2 mM, with between days precision of ≤ 15[%] expressed as relative standard deviation, and an accuracy between days from 88.3[%] to 114.5[%]. This homemade gas diffusion analytical device was successfully used for the determination of ammonia in vitreous humor samples from forensic autopsies. The results obtained with the proposed meth
Ключевые слова:
ammonia; gas diffusion analysis; low cost analytical devices; post mortem interval; vitreous humour
ammonia; indicator; sodium hydroxide; ammonia; coloring agent; thymol blue; thymolphthalein; adult; aged; Article; autopsy; capillary electrophoresis; chemical analysis; controlled study; correlational study; death; gas diffusion; human; human tissue; limit of detection; measurement accuracy; measurement precision; pH; physical phase; priority journal; vitreous body; analogs and derivatives; chemistry; colorimetry; middle aged; postmortem change; reproducibility; smartphone; very elderly; vitreous body; young adult; Adult; Aged; Aged, 80 and over; Ammonia; Colorimetry; Coloring Agents; Humans; Limit of Detection; Middle Aged; Postmortem Changes; Reproducibility of Results; Smartphone; Thymolphthalein; Vitreous Body; Young Adult
Язык текста: Английский
ISSN: 1872-6283
Musile D. Dzhakomo 1985-
Gottardo R.
Palacio C.
Shestakova K. M. Kseniya Mikhaylovna 1992-
Raniero D.
De P. E. F. Palo E.F.
Tal`yaro F. Franko 1952-
Мусиле Д. Джакомо 1985-
Готтардо Р.
Палаcио C.
Шестакова К. М. Ксения Михайловна 1992-
Раниеро Д.
Де П. Е. Ф. Пало Е.Ф.
Тальяро Ф. Франко 1952-
Development of a low cost gas diffusion device for ammonia detection in the vitreous humor and its preliminary application for estimation of the time since death
Development of a low cost gas diffusion device for ammonia detection in the vitreous humor and its preliminary application for estimation of the...
Текст визуальный непосредственный
Forensic Science International
Elsevier Science Publisher B.V.
Vol.295 P. 150-156
2019
Статья
ammonia gas diffusion analysis low cost analytical devices post mortem interval vitreous humour
ammonia indicator sodium hydroxide ammonia coloring agent thymol blue thymolphthalein adult aged Article autopsy capillary electrophoresis chemical analysis controlled study correlational study death gas diffusion human human tissue limit of detection measurement accuracy measurement precision pH physical phase priority journal vitreous body analogs and derivatives chemistry colorimetry middle aged postmortem change reproducibility smartphone very elderly vitreous body young adult Adult Aged Aged, 80 and over Ammonia Colorimetry Coloring Agents Humans Limit of Detection Middle Aged Postmortem Changes Reproducibility of Results Smartphone Thymolphthalein Vitreous Body Young Adult
A simple and low cost analytical device is described for the determination of ammonium in the vitreous humor suitable for inferring the post mortem interval in forensic cases. The device is based on ammonia formation from ammonium ion by means of NaOH addition to the vitreous humor sample and its detection with a pH chemical indicator in the gas phase above the vitreous humor sample. From the gas phase, ammonia diffuses through a polymeric membrane and it is trapped and detected with a droplet of pH indicator thymol blue. The color change of the droplet is measured using a smartphone camera. Under optimal conditions, the device showed a limit of detection of 0.2 mM, with between days precision of ≤ 15[%] expressed as relative standard deviation, and an accuracy between days from 88.3[%] to 114.5[%]. This homemade gas diffusion analytical device was successfully used for the determination of ammonia in vitreous humor samples from forensic autopsies. The results obtained with the proposed meth