Background-Free Fluorescence-Decay-Time Sensing and Imaging of pH with Highly Photostable Diazaoxotriangulenium Dyes
Dalfen I., Dmitriev R. I., Holst G., Klimant I., Borisov S. M.
Analytical Chemistry
Vol.91, Issue1, P. 808-816
Опубликовано: 2019
Тип ресурса: Статья
DOI:10.1021/acs.analchem.8b02534
Аннотация:
Novel fluorescent diazaoxatriangulenium (DAOTA) pH indicators for lifetime-based self-referenced pH sensing are reported. The DAOTA dyes were decorated with phenolic-receptor groups inducing fluorescence quenching via a photoinduced-electron-transfer mechanism. Electron-withdrawing chlorine substituents ensure response in the most relevant pH range (apparent pKa values of ∼5 and 7.5 for the p,p-dichlorophenol- and p-chlorophenol-substituted dyes, respectively). The dyes feature long fluorescence lifetimes (17-20 ns), high quantum yields (∼60[%]), and high photostabilities. Planar optodes are prepared upon immobilization of the dyes into polyurethane hydrogel D4. Apart from the response in the fluorescence intensity, the optodes show pH-dependent lifetime behavior, which makes them suitable for studying 2D pH distributions with the help of fluorescence-lifetime-imaging techniques. The lifetime response is particularly pronounced for the sensors with high dye concentrations (0.5-1 wt [%] wit
Ключевые слова:
Electron transitions; Fluorescence quenching; pH; Dye concentration; Electronwithdrawing; Fluorescence decay time; Fluorescence intensities; Fluorescence lifetime imaging; Fluorescence lifetimes; Photo-induced electron transfer; Sensing and imaging; Fluorescence imaging
Язык текста: Английский
ISSN: 1520-6882
Dalfen I.
Dmitriev R. I. Ruslan Igorevich 1981-
Holst G.
Klimant I.
Borisov S. M.
Далфен И.
Дмитриев Р. И. Руслан Игоревич 1981-
Холст Г.
Климант И.
Борисов С. М.
Background-Free Fluorescence-Decay-Time Sensing and Imaging of pH with Highly Photostable Diazaoxotriangulenium Dyes
Текст визуальный непосредственный
Analytical Chemistry
American Chemical Society
Vol.91, Issue1 P. 808-816
2019
Статья
Electron transitions Fluorescence quenching pH Dye concentration Electronwithdrawing Fluorescence decay time Fluorescence intensities Fluorescence lifetime imaging Fluorescence lifetimes Photo-induced electron transfer Sensing and imaging Fluorescence imaging
Novel fluorescent diazaoxatriangulenium (DAOTA) pH indicators for lifetime-based self-referenced pH sensing are reported. The DAOTA dyes were decorated with phenolic-receptor groups inducing fluorescence quenching via a photoinduced-electron-transfer mechanism. Electron-withdrawing chlorine substituents ensure response in the most relevant pH range (apparent pKa values of ∼5 and 7.5 for the p,p-dichlorophenol- and p-chlorophenol-substituted dyes, respectively). The dyes feature long fluorescence lifetimes (17-20 ns), high quantum yields (∼60[%]), and high photostabilities. Planar optodes are prepared upon immobilization of the dyes into polyurethane hydrogel D4. Apart from the response in the fluorescence intensity, the optodes show pH-dependent lifetime behavior, which makes them suitable for studying 2D pH distributions with the help of fluorescence-lifetime-imaging techniques. The lifetime response is particularly pronounced for the sensors with high dye concentrations (0.5-1 wt [%] wit