Mitochondria-Associated Matrix Metalloproteinases 2 and 9 in Acute Renal Pathologies
Pevzner I. B., Zorova L. D., Galkin F. A., Plotnikov E. Yu., Zorov D. B.
Bulletin of Experimental Biology and Medicine
Vol.166, Issue3, P. 334-338
Опубликовано: 2019
Тип ресурса: Статья
DOI:10.1007/s10517-019-04345-y
Аннотация:
Activities of MMP-2 and MMP-9 in the cytoplasm and mitochondria of kidney cells were evaluated on the models of acute renal pathologies: pyelonephritis, rhabdomyolysis, and ischemia/reperfusion of the kidney. In acute pyelonephritis, a significant increase in the level of MMP-2 and MMP-9 in kidney cells and the appearance of mitochondrial MMP-2 isoform with a lower molecular weight, but still exhibiting proteolytic activity were observed. A direct correlation between the level of MMP-2 and MMP-9 in the kidney and the severity of inflammation in pyelonephritis was revealed. Obviously, the appearance of active protease in the mitochondria of the kidney cells could have an impact on their functioning and, generally, on the fate of renal cells in this pathology. © 2019, Springer Science+Business Media, LLC, part of Springer Nature.
Ключевые слова:
metalloproteinases; mitochondria; oxidative stress; pyelonephritis; regeneration
gelatinase A; gelatinase B; proteinase; gelatinase A; gelatinase B; isoenzyme; Mmp2 protein, rat; Mmp9 protein, rat; acute pyelonephritis; animal cell; Article; controlled study; cytoplasm; enzyme activity; gene expression; H9c2(2-1) cell line; kidney cell; kidney disease; kidney ischemia; mitochondrion; molecular weight; nonhuman; oxidative stress; protein degradation; protein function; rhabdomyolysis; acute disease; animal; bacterial infection; disease model; enzymology; epithelium cell; gene expression regulation; genetics; kidney; metabolism; nephrectomy; outbred strain; pathology; procedures; pyelonephritis; rat; reperfusion injury; severity of illness index; surgery; Acute Disease; Animals; Animals, Outbred Strains; Bacterial Infections; Disease Models, Animal; Epithelial Cells; Gene Expression Regulation; Isoenzymes; Kidney; Matrix Metalloproteinase 2; Matrix Metalloproteinase 9; Mitochondria; Nephrectomy; Pyelonephritis; Rats; Reperfusion Injury; Rhabdomyolysis; Severity of Ill
Язык текста: Английский
ISSN: 1573-8221
Pevzner I. B.
Zorova L. D.
Galkin F. A.
Plotnikov E. Yu. Egor Yuryevich 1980-
Zorov D. B.
Певзнер И. Б.
Зорова Л. Д.
Галкин Ф. А.
Плотников Е. Ю. Егор Юрьевич 1980-
Зоров Д. Б.
Mitochondria-Associated Matrix Metalloproteinases 2 and 9 in Acute Renal Pathologies
Текст визуальный непосредственный
Bulletin of Experimental Biology and Medicine
Springer New York Consultants Bureau
Vol.166, Issue3 P. 334-338
2019
Статья
metalloproteinases mitochondria oxidative stress pyelonephritis regeneration
gelatinase A gelatinase B proteinase gelatinase A gelatinase B isoenzyme Mmp2 protein, rat Mmp9 protein, rat acute pyelonephritis animal cell Article controlled study cytoplasm enzyme activity gene expression H9c2(2-1) cell line kidney cell kidney disease kidney ischemia mitochondrion molecular weight nonhuman oxidative stress protein degradation protein function rhabdomyolysis acute disease animal bacterial infection disease model enzymology epithelium cell gene expression regulation genetics kidney metabolism nephrectomy outbred strain pathology procedures pyelonephritis rat reperfusion injury severity of illness index surgery Acute Disease Animals Animals, Outbred Strains Bacterial Infections Disease Models, Animal Epithelial Cells Gene Expression Regulation Isoenzymes Kidney Matrix Metalloproteinase 2 Matrix Metalloproteinase 9 Mitochondria Nephrectomy Pyelonephritis Rats Reperfusion Injury Rhabdomyolysis Severity of Ill
Activities of MMP-2 and MMP-9 in the cytoplasm and mitochondria of kidney cells were evaluated on the models of acute renal pathologies: pyelonephritis, rhabdomyolysis, and ischemia/reperfusion of the kidney. In acute pyelonephritis, a significant increase in the level of MMP-2 and MMP-9 in kidney cells and the appearance of mitochondrial MMP-2 isoform with a lower molecular weight, but still exhibiting proteolytic activity were observed. A direct correlation between the level of MMP-2 and MMP-9 in the kidney and the severity of inflammation in pyelonephritis was revealed. Obviously, the appearance of active protease in the mitochondria of the kidney cells could have an impact on their functioning and, generally, on the fate of renal cells in this pathology. © 2019, Springer Science+Business Media, LLC, part of Springer Nature.