Antibacterial activity profile of miramistin in in vitro and in vivo models
Agafonova M. N., Kazakova R. R., Lubina A. P., Zeldi M. I., Nikitina E. V., Balakin K. V., Shtyrlin Y. G.
Microbial Pathogenesis
Vol.142, Num.104072
Опубликовано: 2020
Тип ресурса: Статья
DOI:10.1016/j.micpath.2020.104072
Аннотация:
Background: Miramistin is a widely used antiseptic, disinfectant and preservative, and one of the most popular antimicrobial agents on pharmaceutical market of the Russian Federation (http://www.dsm.ru/en/news/385/). However, there is a lack of reported systematic data on antibacterial efficacy of this agent obtained in accordance with the international standards. Aim: This paper represents a systematic study of antibacterial properties of miramistin. Another objective of this work is to evaluate and compare the exploratory performance of in vitro and in vivo protocols of antiseptics’ efficacy testing using miramistin as the reference antiseptic. Methods: Antibacterial activity of 0.1[%] and 0.2[%] aqueous solutions of miramistin against two museum strains of S. aureus (ATCC 209p) and E. coli (CDC F-50) was studied. Three standard in vitro laboratory tests (microdilution test, suspension test, and metal surface test), and one in vivo test (on rat's skin) were used. The study was conducted
Ключевые слова:
in vivo rat skin model; Metal surface test; Minimal bactericidal concentration; Minimal inhibitory concentration; Miramistin; Suspension test
antiinfective agent; bovine serum albumin; miramistin; unclassified drug; animal experiment; animal tissue; antibacterial activity; antimicrobial activity; aqueous solution; Article; bacterial strain; colony forming unit; controlled study; Escherichia coli; in vitro study; in vivo study; inoculation; male; metal surface test; microdilution test; minimum bactericidal concentration; minimum inhibitory concentration; nonhuman; priority journal; rat; skin test; Staphylococcus aureus; suspension test
Язык текста: Английский
ISSN: 1096-1208
Agafonova M. N.
Kazakova R. R.
Lubina A. P.
Zeldi M. I.
Nikitina E. V.
Balakin K. V. Konstantin Valeryevich 1970-
Shtyrlin Y. G.
Агафонова М. Н.
Казакова Р. Р.
Лубина А. П.
Зелди М. И.
Никитина Е. В.
Балакин К. В. Константин Валерьевич 1970-
Штyрлин Y. Г.
Antibacterial activity profile of miramistin in in vitro and in vivo models
Текст визуальный непосредственный
Microbial Pathogenesis
Academic Press
Vol.142 Num.104072
2020
Статья
in vivo rat skin model Metal surface test Minimal bactericidal concentration Minimal inhibitory concentration Miramistin Suspension test
antiinfective agent bovine serum albumin miramistin unclassified drug animal experiment animal tissue antibacterial activity antimicrobial activity aqueous solution Article bacterial strain colony forming unit controlled study Escherichia coli in vitro study in vivo study inoculation male metal surface test microdilution test minimum bactericidal concentration minimum inhibitory concentration nonhuman priority journal rat skin test Staphylococcus aureus suspension test
Background: Miramistin is a widely used antiseptic, disinfectant and preservative, and one of the most popular antimicrobial agents on pharmaceutical market of the Russian Federation (http://www.dsm.ru/en/news/385/). However, there is a lack of reported systematic data on antibacterial efficacy of this agent obtained in accordance with the international standards. Aim: This paper represents a systematic study of antibacterial properties of miramistin. Another objective of this work is to evaluate and compare the exploratory performance of in vitro and in vivo protocols of antiseptics’ efficacy testing using miramistin as the reference antiseptic. Methods: Antibacterial activity of 0.1[%] and 0.2[%] aqueous solutions of miramistin against two museum strains of S. aureus (ATCC 209p) and E. coli (CDC F-50) was studied. Three standard in vitro laboratory tests (microdilution test, suspension test, and metal surface test), and one in vivo test (on rat's skin) were used. The study was conducted