A Novel Microfluidic Device-Based Neurite Outgrowth Inhibition Assay Reveals the Neurite Outgrowth-Promoting Activity of Tropomyosin Tpm3.1 in...
Stefen H., Hassanzadeh-Barforoushi A., Brettle M., Fok S., Suchowerska A. K., Tedla N., Barber T., Ibragimi V. M. M., Fath T.
Cellular and Molecular Neurobiology
Vol.38, Issue8, P. 1557-1563
Опубликовано: 2018
Тип ресурса: Статья
DOI:10.1007/s10571-018-0620-7
Аннотация:
Overcoming neurite inhibition is integral for restoring neuronal connectivity after CNS injury. Actin dynamics are critical for neurite growth cone formation and extension. The tropomyosin family of proteins is a regarded as master regulator of actin dynamics. This study investigates tropomyosin isoform 3.1 (Tpm3.1) as a potential candidate for overcoming an inhibitory substrate, as it is known to influence neurite branching and outgrowth. We designed a microfluidic device that enables neurons to be grown adjacent to an inhibitory substrate, Nogo-66. Results show that neurons, overexpressing hTpm3.1, have an increased propensity to overcome Nogo-66 inhibition. We propose Tpm3.1 as a potential target for promoting neurite growth in an inhibitory environment in the central nervous system. © 2018, Springer Science+Business Media, LLC, part of Springer Nature.
Ключевые слова:
Microfluidic systems; Neurite outgrowth inhibition; NogoA; Tropomyosins
Nogo 66 receptor; tropomyosin; tropomyosin 3.1; unclassified drug; protein Nogo; tropomyosin; Article; brain nerve cell; hippocampus; neurite; neurite outgrowth; priority journal; animal; cytology; drug effect; hippocampus; human; lab on a chip; metabolism; nerve cell; neurite outgrowth; reproducibility; Animals; Hippocampus; Humans; Lab-On-A-Chip Devices; Neuronal Outgrowth; Neurons; Nogo Proteins; Reproducibility of Results; Tropomyosin
Язык текста: Английский
ISSN: 1573-6830
Stefen H.
Hassanzadeh-Barforoushi A.
Brettle M.
Fok S.
Suchowerska A. K.
Tedla N.
Barber T.
Ibragimi V. M. M. Varkiani Madzhid Modzhtaba 1983-
Fath T.
Стефен Х.
Хассанзадех-Барфороуши А.
Бреттле М.
Фок С.
Сучоwерска А. К.
Тедла Н.
Барбер Т.
Ибрагими В. М. М. Варкиани Маджид Моджтаба 1983-
Фатх Т.
A Novel Microfluidic Device-Based Neurite Outgrowth Inhibition Assay Reveals the Neurite Outgrowth-Promoting Activity of Tropomyosin Tpm3.1 in Hippocampal Neurons
A Novel Microfluidic Device-Based Neurite Outgrowth Inhibition Assay Reveals the Neurite Outgrowth-Promoting Activity of Tropomyosin Tpm3.1 in...
Текст визуальный непосредственный
Cellular and Molecular Neurobiology
Springer Science+Business Media B.V., Formerly Kluwer Academic Publishers B.V.
Vol.38, Issue8 P. 1557-1563
2018
Статья
Microfluidic systems Neurite outgrowth inhibition NogoA Tropomyosins
Nogo 66 receptor tropomyosin tropomyosin 3.1 unclassified drug protein Nogo tropomyosin Article brain nerve cell hippocampus neurite neurite outgrowth priority journal animal cytology drug effect hippocampus human lab on a chip metabolism nerve cell neurite outgrowth reproducibility Animals Hippocampus Humans Lab-On-A-Chip Devices Neuronal Outgrowth Neurons Nogo Proteins Reproducibility of Results Tropomyosin
Overcoming neurite inhibition is integral for restoring neuronal connectivity after CNS injury. Actin dynamics are critical for neurite growth cone formation and extension. The tropomyosin family of proteins is a regarded as master regulator of actin dynamics. This study investigates tropomyosin isoform 3.1 (Tpm3.1) as a potential candidate for overcoming an inhibitory substrate, as it is known to influence neurite branching and outgrowth. We designed a microfluidic device that enables neurons to be grown adjacent to an inhibitory substrate, Nogo-66. Results show that neurons, overexpressing hTpm3.1, have an increased propensity to overcome Nogo-66 inhibition. We propose Tpm3.1 as a potential target for promoting neurite growth in an inhibitory environment in the central nervous system. © 2018, Springer Science+Business Media, LLC, part of Springer Nature.