3D in vitro models of tumors expressing EGFR family receptors: a potent tool for studying receptor biology and targeted drug development
Sokolova E. A., Vodeneev V. A., Deev S. M., Balalaeva I. V.
Drug Discovery Today
Vol.24, Issue1, P. 99-111
Опубликовано: 2019
Тип ресурса: Обзор
DOI:10.1016/j.drudis.2018.09.003
Аннотация:
Carcinomas overexpressing EGFR family receptors are of high clinical importance, because the receptors have prognostic value and are used as molecular targets for anticancer therapy. Insufficient drug efficacy necessitates further in-depth research of the receptor biology and improvement in preclinical stages of drug evaluation. Here, we review the currently used advanced 3D in vitro models of tumors, including tumor spheroids, models in natural and synthetic matrices, tumor organoids and microfluidic-based models, as a potent tool for studying EGFR biology and targeted drug development. We are especially focused on factors that affect the biology of tumor cells, causing modification in the expression and basic phosphorylation of the receptors, crosstalk with other signaling pathways and switch between downstream cascades, resulting ultimately in the resistance to antitumor agents. © 2018 Elsevier LtdThis review highlights currently used 3D in vitro models of tumors expressing EGFR fam
Ключевые слова:
antineoplastic agent; epidermal growth factor receptor; cancer resistance; carcinogenesis; decellularization; drug delivery system; drug development; engineered tissue; extracellular matrix; human; hydrogel; in vitro study; microfluidics; molecular biology; molecularly targeted therapy; multicell type tumor spheroid; nonhuman; one cell type tumor spheroid; organoid; protein expression; protein family; protein function; protein modification; protein phosphorylation; receptor cross-talk; Review; signal transduction; three dimensional in vitro tumor model; tumor cell; tumor model; tumor spheroid
Язык текста: Английский
ISSN: 1878-5832
Sokolova E. A.
Vodeneev V. A.
Deev S. M. Sergej Mikhaylovich 1951-
Balalaeva I. V. Irina Vladimirovna 1978-
Соколова Е. А.
Воденеев В. А.
Деев С. М. Сергей Михайлович 1951-
Балалаева И. В. Ирина Владимировна 1978-
3D in vitro models of tumors expressing EGFR family receptors: a potent tool for studying receptor biology and targeted drug development
Текст визуальный непосредственный
Drug Discovery Today
Elsevier Science Publisher B.V.
Vol.24, Issue1 P. 99-111
2019
Обзор
antineoplastic agent epidermal growth factor receptor cancer resistance carcinogenesis decellularization drug delivery system drug development engineered tissue extracellular matrix human hydrogel in vitro study microfluidics molecular biology molecularly targeted therapy multicell type tumor spheroid nonhuman one cell type tumor spheroid organoid protein expression protein family protein function protein modification protein phosphorylation receptor cross-talk Review signal transduction three dimensional in vitro tumor model tumor cell tumor model tumor spheroid
Carcinomas overexpressing EGFR family receptors are of high clinical importance, because the receptors have prognostic value and are used as molecular targets for anticancer therapy. Insufficient drug efficacy necessitates further in-depth research of the receptor biology and improvement in preclinical stages of drug evaluation. Here, we review the currently used advanced 3D in vitro models of tumors, including tumor spheroids, models in natural and synthetic matrices, tumor organoids and microfluidic-based models, as a potent tool for studying EGFR biology and targeted drug development. We are especially focused on factors that affect the biology of tumor cells, causing modification in the expression and basic phosphorylation of the receptors, crosstalk with other signaling pathways and switch between downstream cascades, resulting ultimately in the resistance to antitumor agents. © 2018 Elsevier LtdThis review highlights currently used 3D in vitro models of tumors expressing EGFR fam