Long noncoding RNA HOTAIR is upregulated in an aggressive subgroup of gastrointestinal stromal tumors (GIST) and mediates the establishment of...
Bure I. V., Geer S., Knopf J., Roas M., Henze S., Ströbel P., Agaimy A., Wiemann S., Hoheisel J. D., Hartmann A., Haller F., Moskalev E. A.
Genes Chromosomes and Cancer
Vol.57, Issue11, P. 584-597
Опубликовано: 2018
Тип ресурса: Статья
Аннотация:
Aberrant alterations of DNA methylation are common events in oncogenesis. The origin of cancer-associated epigenetic defects is of interest for mechanistic understanding of malignant transformation and—in the long run—therapeutic modulation of DNA methylation in a locus-specific manner. Given the ability of certain long noncoding RNAs to operate as an interface between DNA and the epigenetic modification machinery which can interact with DNA methyltransferases, we hypothesized—considering HOTAIR as an example—that this transcript may contribute to gene specificity of DNA methylation. Using gastrointestinal stromal tumors (GISTs, n = 67) as a model, we confirmed upregulation of HOTAIR in tumors with high risk of recurrence and showed high abundance of the transcript in GIST cell lines. HOTAIR knockdown in GIST-T1 cells triggered transcriptional response of genes involved in the organization and disassembly of the extracellular matrix and, notably, induced global locus-specific alteratio
Ключевые слова:
DNA methylation; GIST; HOTAIR; long noncoding RNAs
HOTAIR long untranslated RNA, human; long untranslated RNA; adult; aged; chemistry; cohort analysis; DNA methylation; female; gastrointestinal stromal tumor; gastrointestinal tumor; genetics; human; male; middle aged; tumor cell line; upregulation; very elderly; Adult; Aged; Aged, 80 and over; Cell Line, Tumor; Cohort Studies; DNA Methylation; Female; Gastrointestinal Neoplasms; Gastrointestinal Stromal Tumors; Humans; Male; Middle Aged; RNA, Long Noncoding; Up-Regulation
Язык текста: Английский
ISSN: 1098-2264
Bure I. V. Irina Vladimirovna 1988-
Geer S.
Knopf J.
Roas M.
Henze S.
Ströbel P.
Agaimy A.
Wiemann S.
Hoheisel J. D.
Hartmann A.
Haller F.
Moskalev E. A.
Буре И. В. Ирина Владимировна 1988-
Геер С.
Кнопф Й.
Роас М.
Хензе С.
Стрöбел П.
Агаимy А.
Wиеманн С.
Хохеисел Й. Д.
Хартманн А.
Халлер Ф.
Москалев Е. А.
Long noncoding RNA HOTAIR is upregulated in an aggressive subgroup of gastrointestinal stromal tumors (GIST) and mediates the establishment of gene-specific DNA methylation patterns
Long noncoding RNA HOTAIR is upregulated in an aggressive subgroup of gastrointestinal stromal tumors (GIST) and mediates the establishment of...
Текст визуальный непосредственный
Genes Chromosomes and Cancer
Wiley-Liss Inc
Vol.57, Issue11 P. 584-597
2018
Статья
DNA methylation GIST HOTAIR long noncoding RNAs
HOTAIR long untranslated RNA, human long untranslated RNA adult aged chemistry cohort analysis DNA methylation female gastrointestinal stromal tumor gastrointestinal tumor genetics human male middle aged tumor cell line upregulation very elderly Adult Aged Aged, 80 and over Cell Line, Tumor Cohort Studies DNA Methylation Female Gastrointestinal Neoplasms Gastrointestinal Stromal Tumors Humans Male Middle Aged RNA, Long Noncoding Up-Regulation
Aberrant alterations of DNA methylation are common events in oncogenesis. The origin of cancer-associated epigenetic defects is of interest for mechanistic understanding of malignant transformation and—in the long run—therapeutic modulation of DNA methylation in a locus-specific manner. Given the ability of certain long noncoding RNAs to operate as an interface between DNA and the epigenetic modification machinery which can interact with DNA methyltransferases, we hypothesized—considering HOTAIR as an example—that this transcript may contribute to gene specificity of DNA methylation. Using gastrointestinal stromal tumors (GISTs, n = 67) as a model, we confirmed upregulation of HOTAIR in tumors with high risk of recurrence and showed high abundance of the transcript in GIST cell lines. HOTAIR knockdown in GIST-T1 cells triggered transcriptional response of genes involved in the organization and disassembly of the extracellular matrix and, notably, induced global locus-specific alteratio