Unmodified hydrated С60 fullerene molecules exhibit antioxidant properties, prevent damage to DNA and proteins induced by reactive oxygen species...
Gudkov S. V., Guryev E. L., Gapeyev A. B., Sharapov M. G., Bunkin N. F., Shkirin A. V., Zabelina T. S., Glinushkin A. P., Sevost'yanov M. A., Belosludtsev K. N., Chernikov A. V., Bruskov V. I., Zvyagin A. V.
Nanomedicine: Nanotechnology, Biology and Medicine
Vol.15, Issue1, P. 37-46
Опубликовано: 2019
Тип ресурса: Статья
DOI:10.1016/j.nano.2018.09.001
Аннотация:
Unmodified hydrated С60 fullerene molecules (C60UHFM) were shown to reduce the formation ROS in water and 8-oxoguanine in DNA upon ionizing radiation impact. C60UHFM efficiently eliminate long-lived protein radicals arising after irradiation. In irradiated mice C60UHFM reduce the rate of single/double-strand DNA breaks and amount of chromosomal breaks. The radioprotective activity of C60UHFM was estimated by the survival rate of animals; the dose modification factor for animal survival was 1.3. Hematological tests showed that C60UHFM injection in mice prior to irradiation results in a decrement of irradiation-induced leucopenia and thrombocytopenia. Histological analysis testified that C60UHFM provide significant protection of small intestine tissues in mice against irradiation-induced damage. The obtained data assume that the radioprotective properties of C60UHFM are determined by their antioxidant, antiradical and DNA-protective qualities. Thus, it was demonstrated that C60UHFM are a
Ключевые слова:
DNA damage; Fullerene C60; Nanostuctures; Radioprotectors; Reactive oxygen species; X-rays
Antioxidants; DNA; Hydration; Ionizing radiation; Irradiation; Mammals; Oxygen; Proteins; Radiation effects; Radiation shielding; X rays; DNA damages; Fullerene C60; Nanostuctures; Radioprotectors; Reactive oxygen species; Fullerenes; 8 hydroxyguanine; antioxidant; DNA; fullerene derivative; hydroxyl radical; radioprotective agent; reactive oxygen metabolite; unclassified drug; unmodified hydrated c 60 fullerene molecule; water; antioxidant; fullerene C60; fullerene derivative; protein; radioprotective agent; reactive oxygen metabolite; animal experiment; animal model; animal tissue; Article; blood examination; blood vessel injury; chromosome breakage; controlled study; DNA damage; double stranded DNA break; drug dose comparison; drug efficacy; drug mechanism; heart injury; histopathology; in vitro study; intestine injury; ionizing radiation; leukopenia; lung hemorrhage; lung injury; male; mouse; nonhuman; oxidative stress; pericardium; radiation dose; radiation injury; radiation prote
Язык текста: Английский
ISSN: 1549-9642
Gudkov S. V.
Guryev E. L.
Gapeyev A. B.
Sharapov M. G.
Bunkin N. F.
Shkirin A. V.
Zabelina T. S.
Glinushkin A. P.
Sevost'yanov M. A.
Belosludtsev K. N.
Chernikov A. V.
Bruskov V. I.
Zvyagin A. V. Andrej Vasilyevich 1961-
Гудков С. В.
Гурьев Е. Л.
Гапеьев А. Б.
Шарапов М. Г.
Бункин Н. Ф.
Шкирин А. В.
Забелина Т. С.
Глинушкин А. П.
Севост'янов М. А.
Белослудцев К. Н.
Черников А. В.
Брусков В. И.
Звягин А. В. Андрей Васильевич 1961-
Unmodified hydrated С60 fullerene molecules exhibit antioxidant properties, prevent damage to DNA and proteins induced by reactive oxygen species and protect mice against injuries caused by radiation-induced oxidative stress
Unmodified hydrated С60 fullerene molecules exhibit antioxidant properties, prevent damage to DNA and proteins induced by reactive oxygen species...
Текст визуальный непосредственный
Nanomedicine: Nanotechnology, Biology and Medicine
Elsevier Science Publisher B.V.
Vol.15, Issue1 P. 37-46
2019
Статья
DNA damage Fullerene C60 Nanostuctures Radioprotectors Reactive oxygen species X-rays
Antioxidants DNA Hydration Ionizing radiation Irradiation Mammals Oxygen Proteins Radiation effects Radiation shielding X rays DNA damages Fullerene C60 Nanostuctures Radioprotectors Reactive oxygen species Fullerenes 8 hydroxyguanine antioxidant DNA fullerene derivative hydroxyl radical radioprotective agent reactive oxygen metabolite unclassified drug unmodified hydrated c 60 fullerene molecule water antioxidant fullerene C60 fullerene derivative protein radioprotective agent reactive oxygen metabolite animal experiment animal model animal tissue Article blood examination blood vessel injury chromosome breakage controlled study DNA damage double stranded DNA break drug dose comparison drug efficacy drug mechanism heart injury histopathology in vitro study intestine injury ionizing radiation leukopenia lung hemorrhage lung injury male mouse nonhuman oxidative stress pericardium radiation dose radiation injury radiation prote
Unmodified hydrated С60 fullerene molecules (C60UHFM) were shown to reduce the formation ROS in water and 8-oxoguanine in DNA upon ionizing radiation impact. C60UHFM efficiently eliminate long-lived protein radicals arising after irradiation. In irradiated mice C60UHFM reduce the rate of single/double-strand DNA breaks and amount of chromosomal breaks. The radioprotective activity of C60UHFM was estimated by the survival rate of animals; the dose modification factor for animal survival was 1.3. Hematological tests showed that C60UHFM injection in mice prior to irradiation results in a decrement of irradiation-induced leucopenia and thrombocytopenia. Histological analysis testified that C60UHFM provide significant protection of small intestine tissues in mice against irradiation-induced damage. The obtained data assume that the radioprotective properties of C60UHFM are determined by their antioxidant, antiradical and DNA-protective qualities. Thus, it was demonstrated that C60UHFM are a