Аннотация:
Conjugates of Streptococcus pneumoniae type 9N capsular polysaccharide with tetanus toxoid have been prepared. Their interactions with specific antibodies to tetanus toxoid and polysaccharide 9N were assessed, which permitted to select a conjugate with the optimal ratio of the polysaccharide and carrier protein (3:4). The parameters of the efficient adsorption of tetanus toxoid and polysaccharide on aluminum hydroxide for the following in vivo experiments were determined using the quantitative ELISA; in particular, the optimum ratio of the conjugate and adjuvant was proved be ≤ 1:1 (w/w). The repetitive immunization with the selected conjugate adsorbed on aluminum hydroxide increased the anti-PS antibody titers up to 70400 (p < 0,001) which is 140-fold higher as compared to the antigen unconjugated form. The investigation of the antibody protection from Streptococcus pneumoniae 9N after the intranasal challenge of wild-type mice revealed that the titer of the bacterial contamination in
Нуриев Р. И. Ринат Ильшатович 1996-
Галвидис И. А.
Ястребова Н. Е.
Буркин М. А.
Nuriev R. I. Rinat Il`shatovich 1996-
Galvidis I. A.
Yastrebova N. E.
Burkin M. A.
Immunochemical and protective properties of Conjugated Capsular polysaccharide of Streptococcus pneumoniae Serotype 9N [Иммунохимические и протективные свойства конъюгированного капсульного полисахарида Streptococcus pneumoniae Serotype 9N]
Immunochemical and protective properties of Conjugated Capsular polysaccharide of Streptococcus pneumoniae Serotype 9N [Иммунохимические и...
Текст визуальный непосредственный
Биотехнология
Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов Национального исследовательского центра Курчатовски
Т. 34, Вып. 3 С. 33-41
2018
Статья
Capsular polysaccharide Glycoconjugate Pneumococcal vaccine Streptococcus pneumoniae Streptococcus pneumoniae гликоконъюгат Капсульный полисахарид Пневмококковая вакцина
Conjugates of Streptococcus pneumoniae type 9N capsular polysaccharide with tetanus toxoid have been prepared. Their interactions with specific antibodies to tetanus toxoid and polysaccharide 9N were assessed, which permitted to select a conjugate with the optimal ratio of the polysaccharide and carrier protein (3:4). The parameters of the efficient adsorption of tetanus toxoid and polysaccharide on aluminum hydroxide for the following in vivo experiments were determined using the quantitative ELISA; in particular, the optimum ratio of the conjugate and adjuvant was proved be ≤ 1:1 (w/w). The repetitive immunization with the selected conjugate adsorbed on aluminum hydroxide increased the anti-PS antibody titers up to 70400 (p < 0,001) which is 140-fold higher as compared to the antigen unconjugated form. The investigation of the antibody protection from Streptococcus pneumoniae 9N after the intranasal challenge of wild-type mice revealed that the titer of the bacterial contamination in