4-Methylamino antipyrine determination in human plasma by high-performance liquid chromatography tandem mass spectrometry
Nikitina A., Gil`deeva G. N., Grigoriev A., Sidorova A.
Biomedical Chromatography
Vol.34, Issue10, Num.e4913
Опубликовано: 2020
Тип ресурса: Статья
Аннотация:
In the present study, a simple and rapid method for metamizole metabolite 4-methylamino antipyrine (MAA) determination in human plasma was developed, validated and successfully applied to a clinical trial. Chromatographic separation was achieved in HILIC mode on a YMC-Pack SIL column (100 × 2.0 mm; S-5 μm, 30 nm), with a mobile phase consisting of acetonitrile, water and formic acid. Protein precipitation of a small plasma volume using acetonitrile was selected for sample preparation. The multiple reaction monitoring transitions in the positive ionization mode were m/z 218.2 → 56.2 for MAA and m/z 221.2 → 56.2 for MAA-d3 (IS, internal standard). Concentration levels of MAA calibration standards were in the range of 0.100–20 μg/ml. Metamizole conversion into MAA in both water and organic media was investigated, and the level of the conversion in commercially available injection solutions was estimated. © 2020 John Wiley & Sons, Ltd.
Ключевые слова:
4-methylamino antipyrine; HPLC–MS/MS; human plasma; hydrolyzation-oxidation reaction; metamizole
Язык текста: Английский
ISSN: 1099-0801
Nikitina A.
Gil`deeva G. N. Ge`liya Nyazy'fovna 1967-
Grigoriev A.
Sidorova A.
Никитина А.
Гильдеева Г. Н. Гэлия Нязыфовна 1967-
Григориев А.
Сидорова А.
4-Methylamino antipyrine determination in human plasma by high-performance liquid chromatography tandem mass spectrometry
Текст визуальный непосредственный
Biomedical Chromatography
John Wiley & Sons
Vol.34, Issue10 Num.e4913
2020
Статья
4-methylamino antipyrine HPLC–MS/MS human plasma hydrolyzation-oxidation reaction metamizole
In the present study, a simple and rapid method for metamizole metabolite 4-methylamino antipyrine (MAA) determination in human plasma was developed, validated and successfully applied to a clinical trial. Chromatographic separation was achieved in HILIC mode on a YMC-Pack SIL column (100 × 2.0 mm; S-5 μm, 30 nm), with a mobile phase consisting of acetonitrile, water and formic acid. Protein precipitation of a small plasma volume using acetonitrile was selected for sample preparation. The multiple reaction monitoring transitions in the positive ionization mode were m/z 218.2 → 56.2 for MAA and m/z 221.2 → 56.2 for MAA-d3 (IS, internal standard). Concentration levels of MAA calibration standards were in the range of 0.100–20 μg/ml. Metamizole conversion into MAA in both water and organic media was investigated, and the level of the conversion in commercially available injection solutions was estimated. © 2020 John Wiley & Sons, Ltd.