Three-dimensional TCP scaffolds enriched with erythropoietin for stimulation of vascularization and bone formation
Kharkova N. V., Reshetov I. V., Zelyanin A. S., Philippov V. V., Sergeeva N. S., Sviridova I. K., Komlev V. S., Andreeva U. U., Kuznecova O. A.
Electronic Journal of General Medicine
Vol.16, Issue2, Num.em115
Опубликовано: 2019
Тип ресурса: Статья
Аннотация:
In our work, we conducted studies to evaluate the reconstruction of an artificially created critical radial defect in rats with the use of tri-calcium phosphate scaffold enriched with erythropoietin (???). A model of the bone defect on the radius forearm in rats of a critical size has been developed. This model allows to conduct the study without the use of osteosynthesis. EPO is a well-known hormone which regulates formation of the red blood cells (2, 3). ??? increases the expression of VEGF and promotes angiogenesis (3, 4). Therefore, EPO may have a great potential for use as a growth factor for angiogenesis and play its particular role in the bone tissue regeneration. There are no published studies that describe interactions between EPO and biomaterials for development of the bone tissue. Thus, the purpose of this study was to evaluate the interaction between EPO and tri-calcium phosphate scaffold (TCP) with a well-studied biocompatibility and the given porosity, as well as to deter
Ключевые слова:
Bone tissue engineering; Erythropoietin; Scaffold; Tri-calcium phosphate
collagen type 1; erythropoietin; fibrin; glycosaminoglycan; molecular scaffold; paracetamol; tri calcium phosphate scaffold; unclassified drug; vasculotropin; angiogenesis assay; animal experiment; animal model; animal tissue; Article; biocompatibility; biomechanics; blood vessel; body weight; bone radiography; bone remodeling; bone tissue; clinical evaluation; connective tissue; controlled study; enchondral ossification; fascia; hemostasis; histology; immunohistochemistry; information technology; innervation; intervention study; male; microvasculature; nonhuman; ossification; osteosynthesis; polarization microscopy; postoperative care; protein expression; radius; rat; research; stimulation; three dimensional imaging; tissue engineering; tissue regeneration; trabecular bone; ulna; vascularization; wound closure; X ray analysis
Язык текста: Английский
ISSN: 2516-3507
Kharkova N. V.
Reshetov I. V. Igor` Vladimirovich 1964-
Zelyanin A. S. Aleksandr Sergeevich 1969-
Philippov V. V.
Sergeeva N. S.
Sviridova I. K.
Komlev V. S.
Andreeva U. U.
Kuznecova O. A.
Харкова Н. В.
Решетов И. В. Игорь Владимирович 1964-
Зелянин А. С. Александр Сергеевич 1969-
Пхилиппов В. В.
Сергеева Н. С.
Свиридова И. К.
Комлев В. С.
Андреева У. У.
Кузнеcова О. А.
Three-dimensional TCP scaffolds enriched with erythropoietin for stimulation of vascularization and bone formation
Текст визуальный непосредственный
Electronic Journal of General Medicine
Modestum Ltd.
Vol.16, Issue2 Num.em115
2019
Статья
Bone tissue engineering Erythropoietin Scaffold Tri-calcium phosphate
collagen type 1 erythropoietin fibrin glycosaminoglycan molecular scaffold paracetamol tri calcium phosphate scaffold unclassified drug vasculotropin angiogenesis assay animal experiment animal model animal tissue Article biocompatibility biomechanics blood vessel body weight bone radiography bone remodeling bone tissue clinical evaluation connective tissue controlled study enchondral ossification fascia hemostasis histology immunohistochemistry information technology innervation intervention study male microvasculature nonhuman ossification osteosynthesis polarization microscopy postoperative care protein expression radius rat research stimulation three dimensional imaging tissue engineering tissue regeneration trabecular bone ulna vascularization wound closure X ray analysis
In our work, we conducted studies to evaluate the reconstruction of an artificially created critical radial defect in rats with the use of tri-calcium phosphate scaffold enriched with erythropoietin (???). A model of the bone defect on the radius forearm in rats of a critical size has been developed. This model allows to conduct the study without the use of osteosynthesis. EPO is a well-known hormone which regulates formation of the red blood cells (2, 3). ??? increases the expression of VEGF and promotes angiogenesis (3, 4). Therefore, EPO may have a great potential for use as a growth factor for angiogenesis and play its particular role in the bone tissue regeneration. There are no published studies that describe interactions between EPO and biomaterials for development of the bone tissue. Thus, the purpose of this study was to evaluate the interaction between EPO and tri-calcium phosphate scaffold (TCP) with a well-studied biocompatibility and the given porosity, as well as to deter