A new approach to purification of bacterial cellulose membranes: What happens to bacteria in supercritical media?
Pigaleva M. A., Bulat M. V., Gromovy'kh T. I., Gavryushina I. A., Lutsenko S. V., Gallyamov M. O., Novikov I. V., Buyanovskaya A. G., Kiselyova O. I.
The Journal of Supercritical Fluids
Vol.147, P. 59-69
Опубликовано: 2019
Тип ресурса: Статья
DOI:10.1016/j.supflu.2019.02.009
Аннотация:
In this paper, we report our attempts to elaborate the method of purification and simultaneous sterilization of bacterial cellulose films by means of processing them under high pressure, including supercritical conditions for carbon dioxide, for future application in medicine. Bacterial cellulose films were produced by Gluconacetobacter hansenii. The processed samples were controlled by means of AFM, SEM, EDS, IR spectrometry, elemental and microbiological analysis. It was shown that conventional methods do not always provide complete removal of cells and endotoxins and the samples contain traces of compounds used for washing. It was revealed that the outer lipopolysaccharide membrane of the producent cells was destroyed during processing, but bacterial cells still preserved their shape and dimensions. The processed samples were sterile; the level of endotoxins was strongly reduced, though, unfortunately the degree of purification required for medical use was not achieved. © 2019 Elsev
Ключевые слова:
Atomic force microscopy; Bacterial cellulose; Endotoxins; Gluconacetobacter hansenii; Supercritical processing
Air purification; Atomic force microscopy; Carbon dioxide; Carbon films; Cellulose; Supercritical fluid extraction; Bacterial cellulose; Bacterial cellulose membranes; Conventional methods; Endotoxins; Gluconacetobacter hansenii; Microbiological analysis; Supercritical condition; Supercritical processing; Cellulose films; Air Conditioning; Carbon Dioxide; Cells; Cellulose; Medicine; Processing; Purification; Samples
Язык текста: Английский
ISSN: 1872-8162
Pigaleva M. A.
Bulat M. V.
Gromovy'kh T. I. Tat`yana Il`inichna 1950-
Gavryushina I. A. Irina Aleksandrovna 1994-
Lutsenko S. V. Sergej Viktorovich 1957-
Gallyamov M. O.
Novikov I. V.
Buyanovskaya A. G.
Kiselyova O. I.
Пигалева М. А.
Булат М. В.
Громовых Т. И. Татьяна Ильинична 1950-
Гаврюшина И. А. Ирина Александровна 1994-
Луценко С. В. Сергей Викторович 1957-
Галлямов М. О.
Новиков И. В.
Буяновскайа А. Г.
Киселёва О. И.
A new approach to purification of bacterial cellulose membranes: What happens to bacteria in supercritical media?
Текст визуальный непосредственный
The Journal of Supercritical Fluids
Elsevier Science Publisher B.V.
Vol.147 P. 59-69
2019
Статья
Atomic force microscopy Bacterial cellulose Endotoxins Gluconacetobacter hansenii Supercritical processing
Air purification Atomic force microscopy Carbon dioxide Carbon films Cellulose Supercritical fluid extraction Bacterial cellulose Bacterial cellulose membranes Conventional methods Endotoxins Gluconacetobacter hansenii Microbiological analysis Supercritical condition Supercritical processing Cellulose films Air Conditioning Carbon Dioxide Cells Cellulose Medicine Processing Purification Samples
In this paper, we report our attempts to elaborate the method of purification and simultaneous sterilization of bacterial cellulose films by means of processing them under high pressure, including supercritical conditions for carbon dioxide, for future application in medicine. Bacterial cellulose films were produced by Gluconacetobacter hansenii. The processed samples were controlled by means of AFM, SEM, EDS, IR spectrometry, elemental and microbiological analysis. It was shown that conventional methods do not always provide complete removal of cells and endotoxins and the samples contain traces of compounds used for washing. It was revealed that the outer lipopolysaccharide membrane of the producent cells was destroyed during processing, but bacterial cells still preserved their shape and dimensions. The processed samples were sterile; the level of endotoxins was strongly reduced, though, unfortunately the degree of purification required for medical use was not achieved. © 2019 Elsev