Current possibilities and potential development of molecular enterovirus surveillance. Experience of Russian Federation
Лукашев А. Н., Голицина Л. Н., Вакуленко Ю. А., Ахмадишина Л. В., Романенкова Н. И., Сапега Е. Y., Морозова Н. С., Новикова Н. А., Троценко О. Е., Иванова О. Е.
Инфекция и иммунитет
Т. 8, Вып. 4, С. 452-464
Опубликовано: 2018
Тип ресурса: Обзор
DOI:10.15789/2220-7619-2018-4-452-464
Аннотация:
Enteroviruses are small RNA viruses, which are ubiquitous and commonly cause outbreaks with various clinical manifestations. In 2006, the Program on enterovirus surveillance was approved in the Russian Federation. Over the last years, molecular-biological and bioinformatics methods for enterovirus epidemiology studies have been developed both in Russia and worldwide. Currently, identification of enteroviruses is carried out by analyzing nucleotide sequence of the full-length VP1 genome region (ca. 900 nt). Routinely, it is sufficient to obtain a partial VP1 genome region sequence (ca. 300 bp) for enteroviruse verification in most cases; however, a more stringent type criterion of 80[%] sequence identity should be used compared to the 75[%] sequence identity cut-off for the complete VP1 genome region. Further sequence analysis may be performed by using Bayesian phylogenetic methods, which allow using molecular clock to trace outbreak emergence. Enteroviruses accumulate about 0.5–1[%] nucleoti
Ключевые слова:
Enterovirus; Epidemiology; Evolution; Meningitis; Phylogenetics; Surveillance
Язык текста: Русский
ISSN: 2313-7398
Лукашев А. Н. Александр Николаевич 1977-
Голицина Л. Н.
Вакуленко Ю. А. Юлия Александровна 1994-
Ахмадишина Л. В. Людмила Васильевна 1983-
Романенкова Н. И.
Сапега Е. Y. Е.Ю.
Морозова Н. С.
Новикова Н. А.
Троценко О. Е.
Иванова О. Е. Ольга Евгеньевна 1955-
Lukashev A. N. Aleksandr Nikolaevich 1977-
Golitsina L. N.
Vakulenko Yu. A. Yuliya Aleksandrovna 1994-
Akhmadishina L. V. Lyudmila Vasilyevna 1983-
Romanenkova N. I.
Sapega E. Y. E.Yu.
Morozova N. S.
Novikova N. A.
Trotsenko O. E.
Ivanova O. E. Ol`ga Evgenyevna 1955-
Current possibilities and potential development of molecular enterovirus surveillance. Experience of Russian Federation
Текст визуальный непосредственный
Инфекция и иммунитет
Санкт-Петербургское региональное отделение Российской ассоциации аллергологов и клинических иммунологов
Т. 8, Вып. 4 С. 452-464
2018
Обзор
Enterovirus Epidemiology Evolution Meningitis Phylogenetics Surveillance
Enteroviruses are small RNA viruses, which are ubiquitous and commonly cause outbreaks with various clinical manifestations. In 2006, the Program on enterovirus surveillance was approved in the Russian Federation. Over the last years, molecular-biological and bioinformatics methods for enterovirus epidemiology studies have been developed both in Russia and worldwide. Currently, identification of enteroviruses is carried out by analyzing nucleotide sequence of the full-length VP1 genome region (ca. 900 nt). Routinely, it is sufficient to obtain a partial VP1 genome region sequence (ca. 300 bp) for enteroviruse verification in most cases; however, a more stringent type criterion of 80[%] sequence identity should be used compared to the 75[%] sequence identity cut-off for the complete VP1 genome region. Further sequence analysis may be performed by using Bayesian phylogenetic methods, which allow using molecular clock to trace outbreak emergence. Enteroviruses accumulate about 0.5–1[%] nucleoti