Podoplanin regulates the migration of mesenchymal stromal cells and their interaction with platelets
Ward L. S. C., Sheriff L., Marshall J. L., Manning J. E., Brill` A., Nash G. B., McGettrick H. M.
Journal of Cell Science
Vol.132, Issue5, Num.jcs222067
Опубликовано: 2019
Тип ресурса: Статья
Аннотация:
Mesenchymal stromal cells (MSCs) upregulate podoplanin at sites of infection, chronic inflammation and cancer. Here, we investigated the functional consequences of podoplanin expression on the migratory potential of MSCs and their interactions with circulating platelets. Expression of podoplanin significantly enhanced the migration of MSCs compared to MSCs lacking podoplanin. Rac-1 inhibition altered the membrane localisation of podoplanin and in turn significantly reduced MSC migration. Blocking Rac-1 activity had no effect on the migration of MSCs lacking podoplanin, indicating that it was responsible for regulation of migration through podoplanin. When podoplanin-expressing MSCs were seeded on the basal surface of a porous filter, they were able to capture platelets perfused over the uncoated apical surface and induce platelet aggregation. Similar microthrombi were observed when endothelial cells (ECs) were co-cultured on the apical surface. Confocal imaging shows podoplanin-express
Ключевые слова:
Endothelial cell; Mesenchymal stromal cell; Migration; Platelet; Podoplanin
lectin receptor; podoplanin; Rac1 protein; recombinant soluble C type lectin like receptor 2; unclassified drug; CLEC1B protein, human; lectin; membrane protein; PDPN protein, human; Rac1 protein; small interfering RNA; Article; cell interaction; cell migration; cellular distribution; coculture; confocal microscopy; controlled study; endothelium cell; human; human cell; mesenchymal stroma cell; microthrombus; nonhuman; phenotype; priority journal; protein expression; protein function; protein interaction; rat; thrombocyte; thrombocyte aggregation; cell culture; cell motion; genetics; mesenchymal stem cell; metabolism; paracrine signaling; pathology; physiology; thrombocyte; thrombosis; vascular endothelium; Blood Platelets; Cell Movement; Cells, Cultured; Endothelium, Vascular; Humans; Lectins, C-Type; Membrane Glycoproteins; Mesenchymal Stem Cells; Microscopy, Confocal; Paracrine Communication; Platelet Aggregation; rac1 GTP-Binding Protein; RNA, Small Interfering; Thrombosis
Язык текста: Английский
ISSN: 1477-9137
Ward L. S. C.
Sheriff L.
Marshall J. L.
Manning J. E.
Brill` A. Aleksandr 1972-
Nash G. B.
McGettrick H. M.
Wард Л. С. C.
Шерифф Л.
Маршалл Й. Л.
Маннинг Й. Е.
Брилль А. Александр 1972-
Наш Г. Б.
МcГеттриcк Х. М.
Podoplanin regulates the migration of mesenchymal stromal cells and their interaction with platelets
Текст визуальный непосредственный
Journal of Cell Science
The Company of Biologists Ltd.
Vol.132, Issue5 Num.jcs222067
2019
Статья
Endothelial cell Mesenchymal stromal cell Migration Platelet Podoplanin
lectin receptor podoplanin Rac1 protein recombinant soluble C type lectin like receptor 2 unclassified drug CLEC1B protein, human lectin membrane protein PDPN protein, human Rac1 protein small interfering RNA Article cell interaction cell migration cellular distribution coculture confocal microscopy controlled study endothelium cell human human cell mesenchymal stroma cell microthrombus nonhuman phenotype priority journal protein expression protein function protein interaction rat thrombocyte thrombocyte aggregation cell culture cell motion genetics mesenchymal stem cell metabolism paracrine signaling pathology physiology thrombocyte thrombosis vascular endothelium Blood Platelets Cell Movement Cells, Cultured Endothelium, Vascular Humans Lectins, C-Type Membrane Glycoproteins Mesenchymal Stem Cells Microscopy, Confocal Paracrine Communication Platelet Aggregation rac1 GTP-Binding Protein RNA, Small Interfering Thrombosis
Mesenchymal stromal cells (MSCs) upregulate podoplanin at sites of infection, chronic inflammation and cancer. Here, we investigated the functional consequences of podoplanin expression on the migratory potential of MSCs and their interactions with circulating platelets. Expression of podoplanin significantly enhanced the migration of MSCs compared to MSCs lacking podoplanin. Rac-1 inhibition altered the membrane localisation of podoplanin and in turn significantly reduced MSC migration. Blocking Rac-1 activity had no effect on the migration of MSCs lacking podoplanin, indicating that it was responsible for regulation of migration through podoplanin. When podoplanin-expressing MSCs were seeded on the basal surface of a porous filter, they were able to capture platelets perfused over the uncoated apical surface and induce platelet aggregation. Similar microthrombi were observed when endothelial cells (ECs) were co-cultured on the apical surface. Confocal imaging shows podoplanin-express