Аннотация:
Decellularized bovine pericardium (DBP)-based biomeshes are the gold standard in reconstructive surgery. In order to prolong their stability after the transplantation, various chemical cross-linking strategies are employed. However, structural and functional properties of the biomeshes differ in dependence on the cross-linker used. Here, we performed a bottom-up study of structural and functional alterations of DBP-based biomeshes following cross-linking with hexamethylene diisocyanate (HMDC), ethylene glycol diglycidyl ether (EGDE), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and genipin. The in vitro cytotoxicity tests supported their clinical applicability. Their structural differences (eg roughness, fibre thickness, pore morphology) were evaluated using the two-photon confocal laser scanning, atomic force, scanning electron and polarized light microscopies. HMDC and EDC samples appeared to be the roughest. Complex mechanical trials indicated the tendency to reduced Young’s
Ivukina E. A. Ekaterina Andreevna 1986-
Istranov L. P. Leonid Prokofyevich 1937-
Istranova E. V. Elena Viktorovna 1951-
Churbanov S. N. Semen Nikolaevich 1992-
Shavkuta B. S. Boris Sergeevich 1992-
Dmitriev R. I. Ruslan Igorevich 1981-
Veryasova N. N. Nadezhda Nikolaevna 1994-
Kotova S. L. Svetlana Leonidovna 1972-
Kurkov A. V. Aleksandr Vitalyevich 1989-
Shekhter A. B. Anatolij Borukhovich 1933-
Timashev P. S. Petr Sergeevich 1978-
Ивукина Е. А. Екатерина Андреевна 1986-
Истранов Л. П. Леонид Прокофьевич 1937-
Истранова Е. В. Елена Викторовна 1951-
Чурбанов С. Н. Семен Николаевич 1992-
Шавкута Б. С. Борис Сергеевич 1992-
Дмитриев Р. И. Руслан Игоревич 1981-
Верясова Н. Н. Надежда Николаевна 1994-
Котова С. Л. Светлана Леонидовна 1972-
Курков А. В. Александр Витальевич 1989-
Шехтер А. Б. Анатолий Борухович 1933-
Тимашев П. С. Петр Сергеевич 1978-
Chemical cross-linking of xenopericardial biomeshes: A bottom-up study of structural and functional correlations
Текст визуальный непосредственный
Xenotransplantation
Munksgaard International Publishers
Vol.26, Issue3 Num.e12506
2019
Статья
biomechanics bovine pericardium cross-linking decellularization reconstructive surgery
1 (3 dimethylaminopropyl) 3 ethylcarbodiimide etoglucid genipin hexamethylene diisocyanate cross linking reagent iridoid anisotropy Article atomic force microscopy confocal laser scanning microscopy controlled study correlational study cross linking cytotoxicity test engineered heart tissue human human cell in vitro study nonhuman pericardium priority journal scanning electron microscopy Young modulus animal bovine materials testing pericardium procedures surgery tissue engineering xenograft Animals Cattle Cross-Linking Reagents Iridoids Materials Testing Pericardium Tissue Engineering Transplantation, Heterologous
Decellularized bovine pericardium (DBP)-based biomeshes are the gold standard in reconstructive surgery. In order to prolong their stability after the transplantation, various chemical cross-linking strategies are employed. However, structural and functional properties of the biomeshes differ in dependence on the cross-linker used. Here, we performed a bottom-up study of structural and functional alterations of DBP-based biomeshes following cross-linking with hexamethylene diisocyanate (HMDC), ethylene glycol diglycidyl ether (EGDE), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and genipin. The in vitro cytotoxicity tests supported their clinical applicability. Their structural differences (eg roughness, fibre thickness, pore morphology) were evaluated using the two-photon confocal laser scanning, atomic force, scanning electron and polarized light microscopies. HMDC and EDC samples appeared to be the roughest. Complex mechanical trials indicated the tendency to reduced Young’s