Evaluation of therapeutic sensitivity of retinal ganglion cells to targeted peptide bioregulator in culture
Аветисов С. Э., Еричев В. П., Фёдоров А. А., Яременко Т. В., Мураховскайа Y. К.
Вестник офтальмологии
Т. 135, Вып. 1, С. 84-89
Опубликовано: 2019
Тип ресурса: Статья
DOI:10.17116/oftalma201913501184
Аннотация:
Purpose-to study how therapeutically sensitive retinal cell culture is to the peptide bioregulator isolated from cattle retina (Retinalamin) in models of glaucomatous optic neuropathy (GON). Material and methods. The cells were isolated from the retinae separated from newborn mice. Cell sheets were disaggregated and transformed into a suspension. Removal of the off-target cell population was done by adding antibodies to deplete the cells with CD48 marker, and magnetic microbeads that attach to them. Selection of ganglion cells and obtainment of its enriched fraction was done by immunomagnetic separation. To assess the toxicity of Retinalamin, a cytotoxic test was performed on the culture of skin fibroblasts with sequential dilution of the drug into concentrations of 5.0-0.009 mg/mL. The cells were seeded at 5000 per plate well and exposed to the drug for 24 hours. To study the excitotoxic damage, the first group of plate wells with retinal cells had solution of sodium glutamate added i
Ключевые слова:
Excitotoxicity; Glaucoma; Neuroprotection; Retinal ganglion cells
glutamic acid; peptide; animal; drug effect; mouse; physiology; retina ganglion cell; Animals; Glutamic Acid; Mice; Peptides; Retinal Ganglion Cells
Язык текста: Русский
ISSN: 2309-1282
Аветисов С. Э. Сергей Эдуардович 1950-
Еричев В. П.
Фёдоров А. А.
Яременко Т. В. Тамара Владимировна 1991-
Мураховскайа Y. К.
Avetisov S. E`. Sergej E`duardovich 1950-
Erichev V. P.
Fyodorov A. A.
Yaremenko T. V. Tamara Vladimirovna 1991-
Murakhovskaya Y. K.
Evaluation of therapeutic sensitivity of retinal ganglion cells to targeted peptide bioregulator in culture
Текст визуальный непосредственный
Вестник офтальмологии
Издательство Медиа Сфера
Т. 135, Вып. 1 С. 84-89
2019
Статья
Excitotoxicity Glaucoma Neuroprotection Retinal ganglion cells
glutamic acid peptide animal drug effect mouse physiology retina ganglion cell Animals Glutamic Acid Mice Peptides Retinal Ganglion Cells
Purpose-to study how therapeutically sensitive retinal cell culture is to the peptide bioregulator isolated from cattle retina (Retinalamin) in models of glaucomatous optic neuropathy (GON). Material and methods. The cells were isolated from the retinae separated from newborn mice. Cell sheets were disaggregated and transformed into a suspension. Removal of the off-target cell population was done by adding antibodies to deplete the cells with CD48 marker, and magnetic microbeads that attach to them. Selection of ganglion cells and obtainment of its enriched fraction was done by immunomagnetic separation. To assess the toxicity of Retinalamin, a cytotoxic test was performed on the culture of skin fibroblasts with sequential dilution of the drug into concentrations of 5.0-0.009 mg/mL. The cells were seeded at 5000 per plate well and exposed to the drug for 24 hours. To study the excitotoxic damage, the first group of plate wells with retinal cells had solution of sodium glutamate added i