Аннотация:
Marine organisms produce an array of biologically active natural products, many of which have unique structures that have not been found in terrestrial organisms. Hence, marine algae provide a unique source of bioactive compounds. The present study investigated 19 marine algae and one seagrass collected from Torquay beach, Victoria, Australia. High-performance thin-layer chromatography (HPTLC) hyphenated with microchemical (DPPH•, p-anisaldehyde, and Fast Blue B) and biochemical (α-amylase and acetylcholine esterase (AChE) enzymatic) derivatizations was used to evaluate antioxidant activity, presence of phytosterols and phenolic lipids, α-amylase and AChE inhibitory activities of extract components. Significant α-amylase and AChE inhibitory activities were observed in samples 2, 6, 8 and 10. Antioxidant activities in the samples were found to be correlated to phytosterol content (R 2 = 0.78), but was not found to be related to either α-amylase or AChE inhibitory activities. α-Amylase i
Ключевые слова:
Antioxidants; Cholinesterase inhibitors; Effect-directed analysis (EDA); HPTLC; Marine algae; Phenolic lipids; α-amylase inhibitors
1,1 diphenyl 2 picrylhydrazyl; acetylcholinesterase; algal extract; amylase; anisaldehyde; fast blue; phytosterol; resorcinol; sitosterol; antioxidant; biological product; biphenyl derivative; cholinesterase inhibitor; phytosterol; plant extract; antioxidant activity; Article; Australia; biochemistry; biological activity; chemistry; controlled study; enzyme inhibition; high performance thin layer chromatography; limit of detection; limit of quantitation; marine alga; marine species; nonhuman; seagrass; antagonists and inhibitors; chemistry; enzyme assay; high performance liquid chromatography; isolation and purification; metabolism; procedures; seaweed; thin layer chromatography; Acetylcholinesterase; alpha-Amylases; Antioxidants; Australia; Biological Products; Biphenyl Compounds; Cholinesterase Inhibitors; Chromatography, High Pressure Liquid; Chromatography, Thin Layer; Enzyme Assays; Phytosterols; Plant Extracts; Seaweed
Agatonovich-Kushtrin S. Snezhana 1960-
Kustrin E.
Gegechkori V. I. Vladimir Iraklievich 1991-
Morton D. U. De`vid Uil`yam 1959-
Агатонович-Куштрин С. Снежана 1960-
Кустрин Е.
Гегечкори В. И. Владимир Ираклиевич 1991-
Мортон Д. У. Дэвид Уильям 1959-
High-performance thin-layer chromatography hyphenated with microchemical and biochemical derivatizations in bioactivity profiling of marine species
Текст визуальный непосредственный
Marine Drugs
Molecular Diversity Preservation International
Vol.17, Issue3 Num.148
2019
Статья
Antioxidants Cholinesterase inhibitors Effect-directed analysis (EDA) HPTLC Marine algae Phenolic lipids α-amylase inhibitors
1,1 diphenyl 2 picrylhydrazyl acetylcholinesterase algal extract amylase anisaldehyde fast blue phytosterol resorcinol sitosterol antioxidant biological product biphenyl derivative cholinesterase inhibitor phytosterol plant extract antioxidant activity Article Australia biochemistry biological activity chemistry controlled study enzyme inhibition high performance thin layer chromatography limit of detection limit of quantitation marine alga marine species nonhuman seagrass antagonists and inhibitors chemistry enzyme assay high performance liquid chromatography isolation and purification metabolism procedures seaweed thin layer chromatography Acetylcholinesterase alpha-Amylases Antioxidants Australia Biological Products Biphenyl Compounds Cholinesterase Inhibitors Chromatography, High Pressure Liquid Chromatography, Thin Layer Enzyme Assays Phytosterols Plant Extracts Seaweed
Marine organisms produce an array of biologically active natural products, many of which have unique structures that have not been found in terrestrial organisms. Hence, marine algae provide a unique source of bioactive compounds. The present study investigated 19 marine algae and one seagrass collected from Torquay beach, Victoria, Australia. High-performance thin-layer chromatography (HPTLC) hyphenated with microchemical (DPPH•, p-anisaldehyde, and Fast Blue B) and biochemical (α-amylase and acetylcholine esterase (AChE) enzymatic) derivatizations was used to evaluate antioxidant activity, presence of phytosterols and phenolic lipids, α-amylase and AChE inhibitory activities of extract components. Significant α-amylase and AChE inhibitory activities were observed in samples 2, 6, 8 and 10. Antioxidant activities in the samples were found to be correlated to phytosterol content (R 2 = 0.78), but was not found to be related to either α-amylase or AChE inhibitory activities. α-Amylase i