Viscoll collagen solution as a novel bioink for direct 3D bioprinting
Osidak E. O., Karalkin P. A., Osidak M. S., Parfenov V. A., Sivogrivov D. E., Pereira F. D. A. S., Gryadunova A. A., Koudan E. V., Khesuani Y. D., Касянов В. А., Belousov S. I., Krasheninnikov S. V., Grigoriev T. E., Chvalun S. N., Bulanova E. A., Mironov V. A., Domogatsky S. P.
Journal of Materials Science: Materials in Medicine
Vol.30, Issue3, Num.31
Опубликовано: 2019
Тип ресурса: Статья
DOI:10.1007/s10856-019-6233-y
Аннотация:
Collagen is one of the most promising materials for 3D bioprinting because of its distinguished biocompatibility. Cell-laden constructs made of pure collagen with or without incorporated growth supplements support engineered constructs persistence in culture and are perfectly suitable for grafting. The limiting factor for direct 3D collagen printing was poor printability of collagen solutions, especially admixed with cells or tissue spheroids. In our study, we showed that concentrated solutions of native collagen branded Viscoll were effective as bioinks with high fidelity performance. Viscoll containing 20, 30, or 40 mg/ml collagen were used for direct extrusion 3D bioprinting to form scaffolds appropriate to support spatial arrangement of tissue spheroids into rigid patterns with resolution of 0.5 mm in details. Incorporated cells demonstrated sufficient viability. Associated rheological study showed that good printability of the collagen solutions correlates with their increased sto
Ключевые слова:
Biocompatibility; Collagen; Scaffolds (biology); Tissue; Concentrated solution; Direct extrusion; High-fidelity; Native collagen; Physical parameters; Rheological studies; Spatial arrangements; Technological criteria; 3D printers; collagen; unclassified drug; viscoll; biomaterial; collagen; Article; biocompatibility; biomechanics; bioprinting; cell viability; collagen degradation; concentration (parameter); flow kinetics; geometry; physical parameters; priority journal; three dimensional printing; animal; bioprinting; cell survival; chemistry; drug development; human; hydrogel; materials testing; mechanical stress; mouse; multicellular spheroid; NIH 3T3 cell line; pressure; procedures; regenerative medicine; tissue engineering; tissue scaffold; Animals; Biocompatible Materials; Bioprinting; Cell Survival; Collagen; Drug Discovery; Humans; Hydrogels; Materials Testing; Mice; NIH 3T3 Cells; Pressure; Printing, Three-Dimensional; Regenerative Medicine; Rheology; Spheroids, Cellular; Stres
Язык текста: Английский
ISSN: 1573-4838
Osidak E. O.
Karalkin P. A.
Osidak M. S.
Parfenov V. A.
Sivogrivov D. E.
Pereira F. D. A. S.
Gryadunova A. A. Anna Aleksandrovna 1992-
Koudan E. V.
Khesuani Y. D.
Касянов В. А.
Belousov S. I.
Krasheninnikov S. V.
Grigoriev T. E.
Chvalun S. N.
Bulanova E. A.
Mironov V. A. Vladimir Aleksandrovich 1954-
Domogatsky S. P.
Осидак Е. О.
Каралкин П. А.
Осидак М. С.
Парфенов В. А.
Сивогривов Д. Е.
Переира Ф. Д. А. С.
Грядунова А. А. Анна Александровна 1992-
Коудан Е. В.
Хесуани Y. Д.
Kasyanov V. A.
Белоусов С. И.
Крашенинников С. В.
Григориев Т. Е.
Чвалун С. Н.
Буланова Е. А.
Миронов В. А. Владимир Александрович 1954-
Домогацкy С. П.
Viscoll collagen solution as a novel bioink for direct 3D bioprinting
Текст визуальный непосредственный
Journal of Materials Science: Materials in Medicine
Springer Science+Business Media B.V., Formerly Kluwer Academic Publishers B.V.
Vol.30, Issue3 Num.31
2019
Статья
Biocompatibility Collagen Scaffolds (biology) Tissue Concentrated solution Direct extrusion High-fidelity Native collagen Physical parameters Rheological studies Spatial arrangements Technological criteria 3D printers collagen unclassified drug viscoll biomaterial collagen Article biocompatibility biomechanics bioprinting cell viability collagen degradation concentration (parameter) flow kinetics geometry physical parameters priority journal three dimensional printing animal bioprinting cell survival chemistry drug development human hydrogel materials testing mechanical stress mouse multicellular spheroid NIH 3T3 cell line pressure procedures regenerative medicine tissue engineering tissue scaffold Animals Biocompatible Materials Bioprinting Cell Survival Collagen Drug Discovery Humans Hydrogels Materials Testing Mice NIH 3T3 Cells Pressure Printing, Three-Dimensional Regenerative Medicine Rheology Spheroids, Cellular Stres
Collagen is one of the most promising materials for 3D bioprinting because of its distinguished biocompatibility. Cell-laden constructs made of pure collagen with or without incorporated growth supplements support engineered constructs persistence in culture and are perfectly suitable for grafting. The limiting factor for direct 3D collagen printing was poor printability of collagen solutions, especially admixed with cells or tissue spheroids. In our study, we showed that concentrated solutions of native collagen branded Viscoll were effective as bioinks with high fidelity performance. Viscoll containing 20, 30, or 40 mg/ml collagen were used for direct extrusion 3D bioprinting to form scaffolds appropriate to support spatial arrangement of tissue spheroids into rigid patterns with resolution of 0.5 mm in details. Incorporated cells demonstrated sufficient viability. Associated rheological study showed that good printability of the collagen solutions correlates with their increased sto