Inflammatory metabolites of arahidonic acid in tear fluid in UV-induced corneal damage [ВОСПАЛИТЕЛЬНЫЕ МЕТАБОЛИТЫ АРАХИДОНОВОЙ КИСЛОТЫ В СЛЁЗНОЙ...
Чистяков Д. В., Азбукина Н. В., Гориаинов С. В., Чистяков В. В., Ганчарова О. С., Тиулина В. В., Бакшеева В. Е., Иомдина Е. Н., Пхилиппов П. П., Сергеева М. Г., Сенин И. И., Зерний Е. Ю.
Биомедицинская химия
Т. 65, Вып. 1, С. 33-40
Опубликовано: 2019
Тип ресурса: Статья
DOI:10.18097/PBMC20196501033
Аннотация:
The ultraviolet (UV) B-induced damage of the eye surface of experimental animals (rabbits) includes loss of corneal epithelium, apoptosis of keratocytes and stromal edema. These changes are accompanied by clinically and histologically manifested corneal inflammation, neutrophil infiltration, and exudation of the anterior chamber of the eye. According to mass spectrometric analysis, UV-induced corneal damage is associated with pronounced changes in the lipid composition of tears, including a decrease in the amount of arachidonic acid and prostaglandin E2 and an increase in the concentrations of prostaglandin D2 and its derivative 15d-PGJ2. In addition, it is accompanied by an alteration in the levels of hydroxyeicosate tetraenic acid derivatives, namely upregulation of 12-HETE and downregulation of 5-HETE. The revealed changes indicate the activation of metabolic pathways involving 5-lipoxygenase, 12-lipoxygenase, cyclooxygenase 1 and 2, and prostaglandin-D-synthase. These findings cont
Ключевые слова:
12-HETE; 15d-PGJ2; 5-HETE; Eicosanoids; Keratitis; Keratocyte apoptosis; Prostaglandin D2; Prostaglandin E2; Tear film; UV-induced corneal damage
arachidonic acid; animal; chemistry; cornea; cornea injury; keratitis; lacrimal fluid; Leporidae; metabolism; radiation injury; ultraviolet radiation; Animals; Arachidonic Acid; Cornea; Corneal Injuries; Keratitis; Rabbits; Radiation Injuries; Tears; Ultraviolet Rays
Язык текста: Русский
ISSN: 2310-6905
Чистяков Д. В.
Азбукина Н. В.
Гориаинов С. В.
Чистяков В. В.
Ганчарова О. С.
Тиулина В. В.
Бакшеева В. Е.
Иомдина Е. Н.
Пхилиппов П. П.
Сергеева М. Г.
Сенин И. И.
Зерний Е. Ю. Евгений Юрьевич 1977-
Chistyakov D. V.
Azbukina N. V.
Goriainov S. V.
Chistyakov V. V.
Gancharova O. S.
Tiulina V. V.
Baksheeva V. E.
Iomdina E. N.
Philippov P. P.
Sergeeva M. G.
Senin I. I.
Zernij E. Yu. Evgenij Yuryevich 1977-
Inflammatory metabolites of arahidonic acid in tear fluid in UV-induced corneal damage [ВОСПАЛИТЕЛЬНЫЕ МЕТАБОЛИТЫ АРАХИДОНОВОЙ КИСЛОТЫ В СЛЁЗНОЙ ЖИДКОСТИ ПРИ ИНДУЦИРОВАННОМ УЛЬТРАФИОЛЕТОВЫМ ИЗЛУЧЕНИЕМ ПОВРЕЖДЕНИИ РОГОВИЦЫ]
Inflammatory metabolites of arahidonic acid in tear fluid in UV-induced corneal damage [ВОСПАЛИТЕЛЬНЫЕ МЕТАБОЛИТЫ АРАХИДОНОВОЙ КИСЛОТЫ В СЛЁЗНОЙ...
Текст визуальный непосредственный
Биомедицинская химия
Научно-исследовательский институт биомедицинской химии им. В.Н. Ореховича
Т. 65, Вып. 1 С. 33-40
2019
Статья
12-HETE 15d-PGJ2 5-HETE Eicosanoids Keratitis Keratocyte apoptosis Prostaglandin D2 Prostaglandin E2 Tear film UV-induced corneal damage
arachidonic acid animal chemistry cornea cornea injury keratitis lacrimal fluid Leporidae metabolism radiation injury ultraviolet radiation Animals Arachidonic Acid Cornea Corneal Injuries Keratitis Rabbits Radiation Injuries Tears Ultraviolet Rays
The ultraviolet (UV) B-induced damage of the eye surface of experimental animals (rabbits) includes loss of corneal epithelium, apoptosis of keratocytes and stromal edema. These changes are accompanied by clinically and histologically manifested corneal inflammation, neutrophil infiltration, and exudation of the anterior chamber of the eye. According to mass spectrometric analysis, UV-induced corneal damage is associated with pronounced changes in the lipid composition of tears, including a decrease in the amount of arachidonic acid and prostaglandin E2 and an increase in the concentrations of prostaglandin D2 and its derivative 15d-PGJ2. In addition, it is accompanied by an alteration in the levels of hydroxyeicosate tetraenic acid derivatives, namely upregulation of 12-HETE and downregulation of 5-HETE. The revealed changes indicate the activation of metabolic pathways involving 5-lipoxygenase, 12-lipoxygenase, cyclooxygenase 1 and 2, and prostaglandin-D-synthase. These findings cont