Bladder organoids and spheroids: Potential tools for normal and diseased tissue modelling
Vasyutin I. A., Zerihun L., Ivan C., Atala A.
Anticancer Research
Vol.39, Issue3, P. 1105-1118
Опубликовано: 2019
Тип ресурса: Обзор
DOI:10.21873/anticanres.13219
Аннотация:
Bladder-related diseases are among the most common and costly to the healthcare system, and therefore require new approaches to treatment. Organoids and spheroids are 3D cultures that mimic organ features ex vivo and offer novel approaches for diagnostic and therapeutic assessment. The aim of this article was to provide a systematic review of the literature related to bladder organoids and spheroids, applied to disease diagnosis, characterization, and treatment. PubMed and Web of Science were utilized in March 2018 to compile 191 articles satisfying search criteria related to bladder organoids or spheroids and 58 articles were included in the final review. Finally, cell types and techniques utilized for spheroid and organoid manufacture were characterized. The applications of bladder carcinoma spheroids and organoids followed three themes: cancer characterization, diagnosis, and treatment. Tumor characterization studies included a focus on extracellular matrix, microenvironment, geneti
Ключевые слова:
Bladder; In vitro model; Organoids; Review; Spheroids
hypericin; oncolytic virus; bladder; bladder carcinoma; bladder disease; cancer chemotherapy; cancer immunotherapy; extracellular matrix; gene therapy; human; organoid; photodynamic therapy; priority journal; Review; systematic review; tumor growth; tumor microenvironment; tumor spheroid; urinary tract infection; white light; bladder tumor; cancer stem cell; cell culture technique; multicellular spheroid; physiology; regeneration; urothelium; Cell Culture Techniques; Humans; Neoplastic Stem Cells; Organoids; Regeneration; Spheroids, Cellular; Urinary Bladder; Urinary Bladder Neoplasms; Urothelium
Язык текста: Английский
ISSN: 1791-7530
Vasyutin I. A. Igor` Alekseevich 1989-
Zerihun L.
Ivan C.
Atala A.
Васютин И. А. Игорь Алексеевич 1989-
Зерихун Л.
Иван C.
Атала А.
Bladder organoids and spheroids: Potential tools for normal and diseased tissue modelling
Текст визуальный непосредственный
Anticancer Research
International Institute of Anticancer Research
Vol.39, Issue3 P. 1105-1118
2019
Обзор
Bladder In vitro model Organoids Review Spheroids
hypericin oncolytic virus bladder bladder carcinoma bladder disease cancer chemotherapy cancer immunotherapy extracellular matrix gene therapy human organoid photodynamic therapy priority journal Review systematic review tumor growth tumor microenvironment tumor spheroid urinary tract infection white light bladder tumor cancer stem cell cell culture technique multicellular spheroid physiology regeneration urothelium Cell Culture Techniques Humans Neoplastic Stem Cells Organoids Regeneration Spheroids, Cellular Urinary Bladder Urinary Bladder Neoplasms Urothelium
Bladder-related diseases are among the most common and costly to the healthcare system, and therefore require new approaches to treatment. Organoids and spheroids are 3D cultures that mimic organ features ex vivo and offer novel approaches for diagnostic and therapeutic assessment. The aim of this article was to provide a systematic review of the literature related to bladder organoids and spheroids, applied to disease diagnosis, characterization, and treatment. PubMed and Web of Science were utilized in March 2018 to compile 191 articles satisfying search criteria related to bladder organoids or spheroids and 58 articles were included in the final review. Finally, cell types and techniques utilized for spheroid and organoid manufacture were characterized. The applications of bladder carcinoma spheroids and organoids followed three themes: cancer characterization, diagnosis, and treatment. Tumor characterization studies included a focus on extracellular matrix, microenvironment, geneti