1064 nm Nd:YAG laser light affects transmembrane mitochondria respiratory chain complexes
Ravera S., Ferrando S., Agas D., De A. N., Raffetto M., Sabbieti M. G., Sin`ore A., Benedicenti S., Amaroli A.
Journal of Biophotonics
Vol.12, Issue9, Num.e201900101
Опубликовано: 2019
Тип ресурса: Письмо
DOI:10.1002/jbio.201900101
Аннотация:
Photobiomodulation (PBM) is a non-plant-cell manipulation through a transfer of energy by means of light sources at the non-ablative or thermal intensity. Authors showed that cytochrome-c-oxidase (complex IV) is the specific chromophore's target of PBM at the red (600-700 nm) and NIR (760-900 nm) wavelength regions. Recently, it was suggested that the infrared region of the spectrum could influence other chromospheres, despite the interaction by wavelengths higher than 900 nm with mitochondrial chromophores was not clearly demonstrated. We characterized the interaction between mitochondria respiratory chain, malate dehydrogenase, a key enzyme of Krebs cycle, and 3-hydroxyacyl-CoA dehydrogenase, an enzyme involved in the β-oxidation (two mitochondrial matrix enzymes) with the 1064 nm Nd:YAG (100mps and 10 Hz frequency mode) irradiated at the average power density of 0.50, 0.75, 1.00, 1.25 and 1.50 W/cm2 to generate the respective fluences of 30, 45, 60, 75 and 90 J/cm2. Our results show
Ключевые слова:
adenosine triphosphate (ATP); cytochrome c oxidase; infrared light; low-level laser therapy (LLLT); photobiomodulation
Adenosinetriphosphate; Cell membranes; Chromophores; Energy transfer; Enzymes; Iron compounds; Laser surgery; Mitochondria; Molecular biology; Molecules; Neodymium lasers; Phosphates; Sulfur compounds; Yttrium aluminum garnet; Cytochrome c oxidase; Infrared light; Low level laser therapy; Mitochondrial complex; Mitochondrial matrix; Mitochondrial membranes; Photobiomodulation; Respiratory chain complex; Copper compounds
Язык текста: Английский
ISSN: 1864-0648
Ravera S.
Ferrando S.
Agas D.
De A. N. Angelis N.
Raffetto M.
Sabbieti M. G.
Sin`ore A. Antonio 1971-
Benedicenti S.
Amaroli A. Andrea 1971-
Равера С.
Феррандо С.
Агас Д.
Де А. Н. Ангелис Н.
Раффетто М.
Саббиети М. Г.
Синьоре А. Антонио 1971-
Бенедиcенти С.
Амароли А. Андреа 1971-
1064 nm Nd:YAG laser light affects transmembrane mitochondria respiratory chain complexes
Текст визуальный непосредственный
Journal of Biophotonics
Wiley-VCH
Vol.12, Issue9 Num.e201900101
2019
Письмо
adenosine triphosphate (ATP) cytochrome c oxidase infrared light low-level laser therapy (LLLT) photobiomodulation
Adenosinetriphosphate Cell membranes Chromophores Energy transfer Enzymes Iron compounds Laser surgery Mitochondria Molecular biology Molecules Neodymium lasers Phosphates Sulfur compounds Yttrium aluminum garnet Cytochrome c oxidase Infrared light Low level laser therapy Mitochondrial complex Mitochondrial matrix Mitochondrial membranes Photobiomodulation Respiratory chain complex Copper compounds
Photobiomodulation (PBM) is a non-plant-cell manipulation through a transfer of energy by means of light sources at the non-ablative or thermal intensity. Authors showed that cytochrome-c-oxidase (complex IV) is the specific chromophore's target of PBM at the red (600-700 nm) and NIR (760-900 nm) wavelength regions. Recently, it was suggested that the infrared region of the spectrum could influence other chromospheres, despite the interaction by wavelengths higher than 900 nm with mitochondrial chromophores was not clearly demonstrated. We characterized the interaction between mitochondria respiratory chain, malate dehydrogenase, a key enzyme of Krebs cycle, and 3-hydroxyacyl-CoA dehydrogenase, an enzyme involved in the β-oxidation (two mitochondrial matrix enzymes) with the 1064 nm Nd:YAG (100mps and 10 Hz frequency mode) irradiated at the average power density of 0.50, 0.75, 1.00, 1.25 and 1.50 W/cm2 to generate the respective fluences of 30, 45, 60, 75 and 90 J/cm2. Our results show