Efficacy of eradication therapy with stimbifid plus in experimental acute helicobacter pylori infection in murinemodels and in volunteers
Чичерин И. Y., Погорелскy И. П., Дармов И. В., Лундовских И. А., Шабалина М. Р., Колеватyх Е. П., Козлов П. А., Корнаухов А. С.
Инфекционные болезни
Т. 16, Вып. 4, С. 62-74
Опубликовано: 2018
Тип ресурса: Статья
DOI:10.20953/1729-9225-2018-4-62-74
Аннотация:
Objective: to evaluate the possibility of creating a human model of acute Helicobacter pylori infection in healthy volunteers after infecting them with a mutant rifampicin-resistant strain of H. pylori KM-11 (Rif R ), to obtain evidence of H. pylori survival and invasion into the gastric mucosa, describe the symptoms, and assess the efficacy of H. pylori eradication therapy with Stimbifid plus. Materials and methods. In our experiments, we used conventional white mice of both genders weighing 18–20 g. The concentration of bifidobacteria, lactobacilli, and Escherichia (CFU) in animal faeces was determined by inoculating tenfold dilutions of biomaterial onto solid media and further counting of bacterial colonies grown after the incubation period. Microorganisms were cultivated in an anaerobic incubator and then identified by morphological evaluation and using biochemical identification kits. We created a murine model of H. pylori infection by oral administration of H. pylori KM-11 (Rif R
Ключевые слова:
Acute helicobacter pylori infection; Colonization resistance; Dysbiosis; Eradication; Gastritis; Helicobacter pylori; Microbiota; Stimbifid plus; Volunteers; White mice
Язык текста: Русский
ISSN: 2414-9691
Чичерин И. Y.
Погорелскy И. П.
Дармов И. В.
Лундовских И. А.
Шабалина М. Р.
Колеватyх Е. П.
Козлов П. А. Павел Аркадьевич 1986-
Корнаухов А. С.
Chicherin I. Y.
Pogorelsky I. P.
Darmov I. V.
Lundovskikh I. A.
Shabalina M. R.
Kolevatykh E. P.
Kozlov P. A. Pavel Arkadyevich 1986-
Kornaukhov A. S.
Efficacy of eradication therapy with stimbifid plus in experimental acute helicobacter pylori infection in murinemodels and in volunteers
Текст визуальный непосредственный
Инфекционные болезни
ООО "Издательство "Династия"
Т. 16, Вып. 4 С. 62-74
2018
Статья
Acute helicobacter pylori infection Colonization resistance Dysbiosis Eradication Gastritis Helicobacter pylori Microbiota Stimbifid plus Volunteers White mice
Objective: to evaluate the possibility of creating a human model of acute Helicobacter pylori infection in healthy volunteers after infecting them with a mutant rifampicin-resistant strain of H. pylori KM-11 (Rif R ), to obtain evidence of H. pylori survival and invasion into the gastric mucosa, describe the symptoms, and assess the efficacy of H. pylori eradication therapy with Stimbifid plus. Materials and methods. In our experiments, we used conventional white mice of both genders weighing 18–20 g. The concentration of bifidobacteria, lactobacilli, and Escherichia (CFU) in animal faeces was determined by inoculating tenfold dilutions of biomaterial onto solid media and further counting of bacterial colonies grown after the incubation period. Microorganisms were cultivated in an anaerobic incubator and then identified by morphological evaluation and using biochemical identification kits. We created a murine model of H. pylori infection by oral administration of H. pylori KM-11 (Rif R