Angionorm Complex Herbal Preparation Constituents Identified by High Performance Liquid Chromatography-Mass Spectrometry
Struchkov P. A., Mel`nikov E. S., Beloborodov V. L., Kolkhir V. K., Voskoboinikova I. V.
Pharmaceutical Chemistry Journal
Vol.52, Issue12, P. 980-985
Опубликовано: 2019
Тип ресурса: Статья
DOI:10.1007/s11094-019-01937-w
Аннотация:
Angionorm is an original Russian preparation based on the dried aqueous EtOH (25[%]) extract of a herbal mixture containing horse chestnuts (Aesculus hippocastanum L.), licorice roots (Glycyrrhiza glabra L.), hawthorn fruit (Crataegus), and dog-rose hips (Rosa canina). Areversed-phase HPLC-MS-MS procedure with gradient elution was developed to identify the main constituents in extracts of the mixture and each raw material type. Standards helped to identify 15 compounds of various classes (phenolic and hydroxycinnamic acids and their esters, flavonoids, flavonoid glycosides, triterpene saponins). Peaks of hyperoside, liquiritin, â-glycyrrhizic acid, and escin isomers were identified using MRM transitions, elution order, and literature data. Herbal sources of identified constituents were determined. Marker compounds characteristic of each raw-material type in the mixture extract were proposed. © 2019, Springer Science+Business Media, LLC, part of Springer Nature.
Ключевые слова:
Angionorm; flavonoids; HPLC-MS-MS; polyphenolic compounds; saponins
Angionorm extract; beta escin; beta resorcylic acid; caffeic acid; chlorogenic acid; coumaric acid; ester; flavonoid; flavonoid glycoside; formononetin; gallic acid; glycyrrhizic acid; herbaceous agent; hyperin; isoliquiritigenin; kaempferol; liquiritin; luteolin; plant extract; quercetin; rutoside; triterpene; unclassified drug; Angionorm; Article; biological activity; comparative study; controlled study; density gradient; high performance liquid chromatography; liquid chromatography-mass spectrometry; medicinal plant; multiple reaction monitoring; nonhuman; retention time; reversed phase high performance liquid chromatography
Язык текста: Английский
ISSN: 1573-9031
Struchkov P. A.
Mel`nikov E. S. Evgenij Sergeevich 1990-
Beloborodov V. L. Vladimir Leonidovich 1948-
Kolkhir V. K.
Voskoboinikova I. V.
Стручков П. А.
Мельников Е. С. Евгений Сергеевич 1990-
Белобородов В. Л. Владимир Леонидович 1948-
Колхир В. К.
Воскобоиникова И. В.
Angionorm Complex Herbal Preparation Constituents Identified by High Performance Liquid Chromatography-Mass Spectrometry
Текст визуальный непосредственный
Pharmaceutical Chemistry Journal
Springer New York Consultants Bureau
Vol.52, Issue12 P. 980-985
2019
Статья
Angionorm flavonoids HPLC-MS-MS polyphenolic compounds saponins
Angionorm extract beta escin beta resorcylic acid caffeic acid chlorogenic acid coumaric acid ester flavonoid flavonoid glycoside formononetin gallic acid glycyrrhizic acid herbaceous agent hyperin isoliquiritigenin kaempferol liquiritin luteolin plant extract quercetin rutoside triterpene unclassified drug Angionorm Article biological activity comparative study controlled study density gradient high performance liquid chromatography liquid chromatography-mass spectrometry medicinal plant multiple reaction monitoring nonhuman retention time reversed phase high performance liquid chromatography
Angionorm is an original Russian preparation based on the dried aqueous EtOH (25[%]) extract of a herbal mixture containing horse chestnuts (Aesculus hippocastanum L.), licorice roots (Glycyrrhiza glabra L.), hawthorn fruit (Crataegus), and dog-rose hips (Rosa canina). Areversed-phase HPLC-MS-MS procedure with gradient elution was developed to identify the main constituents in extracts of the mixture and each raw material type. Standards helped to identify 15 compounds of various classes (phenolic and hydroxycinnamic acids and their esters, flavonoids, flavonoid glycosides, triterpene saponins). Peaks of hyperoside, liquiritin, â-glycyrrhizic acid, and escin isomers were identified using MRM transitions, elution order, and literature data. Herbal sources of identified constituents were determined. Marker compounds characteristic of each raw-material type in the mixture extract were proposed. © 2019, Springer Science+Business Media, LLC, part of Springer Nature.