Protective effects of Galium verum L. extract against cardiac ischemia/reperfusion injury in spontaneously hypertensive rats
Bradic J., Zivkovic V., Srejovic I., Yakovlevich V., Petkovic A., Turnic T. N., Jeremic J., Jeremic N., Mitrovic S., Sobot T., Ponorac N., Ravic M., Tomovic M.
Oxidative Medicine and Cellular Longevity
Vol.2019, Num.4235405
Опубликовано: 2019
Тип ресурса: Статья
Аннотация:
Galium verum L. (G. verum, lady’s bedstraw) is a perennial herbaceous plant, belonging to the Rubiaceae family. It has been widely used throughout history due to multiple therapeutic properties. However, the effects of this plant species on functional recovery of the heart after ischemia have still not been fully clarified. Therefore, the aim of our study was to examine the effects of methanol extract of G. verum on myocardial ischemia/reperfusion (I/R) injury in spontaneously hypertensive rats (SHR), with a special emphasis on the role of oxidative stress. Rats involved in the research were divided randomly into two groups: control (spontaneously hypertensive rats (SHR)) and G. verum group, including SHR rats treated with the G. verum extract (500 mg/kg body weight per os) for 4 weeks. At the end of the treatment, in vivo cardiac function was assessed by echocardiography. Rats were sacrificed and blood samples were taken for spectrophotometric determination of systemic redox state. He
Ключевые слова:
Echocardiography; Effluents; Plants (botany); Rats; Cardiac contractility; Functional recovery; Left ventricular hypertrophies; Myocardial ischemia; Spectrophotometric determination; Spontaneously hypertensive rats; Structural damages; Therapeutic properties; Heart; catalase; Galium verum extract; glutathione; hydrogen peroxide; nitrite; plant extract; protective agent; superoxide; superoxide dismutase; thiobarbituric acid reactive substance; unclassified drug; animal experiment; animal model; animal tissue; Article; blood sampling; body weight; cell structure; controlled study; drug effect; echocardiography; enzyme activity; ex vivo study; fractional shortening; Galium; Galium verum; heart function; heart left ventricle hypertrophy; heart left ventricle pressure; heart protection; heart tissue; heart weight; in vivo study; lipid peroxidation; myocardial ischemia reperfusion injury; nonhuman; oxidation reduction state; oxidative stress; rat; spectrophotometry; staining; systolic blood
Язык текста: Английский
ISSN: 1942-0994
Bradic J.
Zivkovic V.
Srejovic I.
Yakovlevich V. Vladimir 1971-
Petkovic A.
Turnic T. N.
Jeremic J.
Jeremic N.
Mitrovic S.
Sobot T.
Ponorac N.
Ravic M.
Tomovic M.
Брадиc Й.
Зивковиc В.
Срейовиc И.
Яковлевич В. Владимир 1971-
Петковиc А.
Тюрниc Т. Н.
Йеремиc Й.
Йеремиc Н.
Митровиc С.
Собот Т.
Понораc Н.
Равиc М.
Томовиc М.
Protective effects of Galium verum L. extract against cardiac ischemia/reperfusion injury in spontaneously hypertensive rats
Текст визуальный непосредственный
Oxidative Medicine and Cellular Longevity
Vol.2019 Num.4235405
2019
Статья
Echocardiography Effluents Plants (botany) Rats Cardiac contractility Functional recovery Left ventricular hypertrophies Myocardial ischemia Spectrophotometric determination Spontaneously hypertensive rats Structural damages Therapeutic properties Heart catalase Galium verum extract glutathione hydrogen peroxide nitrite plant extract protective agent superoxide superoxide dismutase thiobarbituric acid reactive substance unclassified drug animal experiment animal model animal tissue Article blood sampling body weight cell structure controlled study drug effect echocardiography enzyme activity ex vivo study fractional shortening Galium Galium verum heart function heart left ventricle hypertrophy heart left ventricle pressure heart protection heart tissue heart weight in vivo study lipid peroxidation myocardial ischemia reperfusion injury nonhuman oxidation reduction state oxidative stress rat spectrophotometry staining systolic blood
Galium verum L. (G. verum, lady’s bedstraw) is a perennial herbaceous plant, belonging to the Rubiaceae family. It has been widely used throughout history due to multiple therapeutic properties. However, the effects of this plant species on functional recovery of the heart after ischemia have still not been fully clarified. Therefore, the aim of our study was to examine the effects of methanol extract of G. verum on myocardial ischemia/reperfusion (I/R) injury in spontaneously hypertensive rats (SHR), with a special emphasis on the role of oxidative stress. Rats involved in the research were divided randomly into two groups: control (spontaneously hypertensive rats (SHR)) and G. verum group, including SHR rats treated with the G. verum extract (500 mg/kg body weight per os) for 4 weeks. At the end of the treatment, in vivo cardiac function was assessed by echocardiography. Rats were sacrificed and blood samples were taken for spectrophotometric determination of systemic redox state. He