Nano- and Microfibrous Materials Based on Collagen for Tissue Engineering: Synthesis, Structure, and Properties
Tenchurin T. K., Istranov L. P., Istranova E. V., Shepelev A. D., Mamagulashvili V. G., Malakhov S. N., Kamyshinskii R. A., Orekhov A. S., Vasil’ev A. L., Sytina E. V., Krasheninnikov S. V., Chvalun S. N.
Nanotechnologies in Russia
Vol.13, Issue9-10, P. 476-486
Опубликовано: 2018
Тип ресурса: Статья
DOI:10.1134/S1995078018050154
Аннотация:
Abstract: The creation of three-dimensional structures possessing biomimetic properties is of considerable interest for regenerative medicine. Nonwoven materials with a diameter of fibers from 100 nm to 3 µm have been obtained by electrospinning from dispersions of collagen in hexafluoroisopropanol and acetic acid (AA). A study by the circular dichroism method indicates the predominant preservation of a collagen triple helix in produced materials. To preserve the fibrous structure in aqueous media, collagen is cross-linked by genipin in isopropanol and PBS (phosphate buffered saline, pH 7.4). The mechanical and biological properties of cross-linked materials have been studied—it is shown that the mechanical behavior of collagen materials in the physiological range of loads corresponds to the mechanical behavior of the native aorta. Cross-linked fibrous materials do not possess cytotoxicity, and they contribute to the adhesion and proliferation of cells. © 2019, Pleiades Publishing, Ltd
Ключевые слова:
Biomimetics; Collagen; Dichroism; Electrospinning; Tissue engineering; Weaving; Biological properties; Biomimetic properties; Collagen triple helix; Cross-linked materials; Hexafluoroisopropanol; Microfibrous materials; Phosphate-buffered salines; Three-dimensional structure; Biological materials
Язык текста: Английский
ISSN: 1995-0799
Tenchurin T. K.
Istranov L. P. Leonid Prokofyevich 1937-
Istranova E. V. Elena Viktorovna 1951-
Shepelev A. D.
Mamagulashvili V. G.
Malakhov S. N.
Kamyshinskii R. A.
Orekhov A. S.
Vasil’ev A. L.
Sytina E. V.
Krasheninnikov S. V.
Chvalun S. N.
Тенчурин Т. К.
Истранов Л. П. Леонид Прокофьевич 1937-
Истранова Е. В. Елена Викторовна 1951-
Шепелев А. Д.
Мамагулашвили В. Г.
Малахов С. Н.
Камyшинскии Р. А.
Орехов А. С.
Васил’ев А. Л.
Сyтина Е. В.
Крашенинников С. В.
Чвалун С. Н.
Nano- and Microfibrous Materials Based on Collagen for Tissue Engineering: Synthesis, Structure, and Properties
Текст визуальный непосредственный
Nanotechnologies in Russia
Pleiades Publishing, Ltd.
Vol.13, Issue9-10 P. 476-486
2018
Статья
Biomimetics Collagen Dichroism Electrospinning Tissue engineering Weaving Biological properties Biomimetic properties Collagen triple helix Cross-linked materials Hexafluoroisopropanol Microfibrous materials Phosphate-buffered salines Three-dimensional structure Biological materials
Abstract: The creation of three-dimensional structures possessing biomimetic properties is of considerable interest for regenerative medicine. Nonwoven materials with a diameter of fibers from 100 nm to 3 µm have been obtained by electrospinning from dispersions of collagen in hexafluoroisopropanol and acetic acid (AA). A study by the circular dichroism method indicates the predominant preservation of a collagen triple helix in produced materials. To preserve the fibrous structure in aqueous media, collagen is cross-linked by genipin in isopropanol and PBS (phosphate buffered saline, pH 7.4). The mechanical and biological properties of cross-linked materials have been studied—it is shown that the mechanical behavior of collagen materials in the physiological range of loads corresponds to the mechanical behavior of the native aorta. Cross-linked fibrous materials do not possess cytotoxicity, and they contribute to the adhesion and proliferation of cells. © 2019, Pleiades Publishing, Ltd